Any feedback?
Literature summary for 2.7.9.1 extracted from
- Pyruvate,phosphate dikinase from Bacteroides symbiosus and Propionibacterium shermanii (1975), Methods Enzymol., 42C, 199-212.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing inactivates | [Clostridium] symbiosum |
| sensitive to dilution, particularly at concentrations below 0.3 mg/ml, stable to freezing and thawing | Propionibacterium freudenreichii subsp. shermanii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5-dithiobis(2-nitrobenzoic acid) | - |
[Clostridium] symbiosum |  |
| 5,5-dithiobis(2-nitrobenzoic acid) | - |
Propionibacterium freudenreichii subsp. shermanii | |
| alpha-beta-methylene ATP | competitive to ATP | [Clostridium] symbiosum | |
| alpha-beta-methylene ATP | competitive to ATP | Propionibacterium freudenreichii subsp. shermanii | |
| beta-gamma-methylene ATP | competitive to ATP | [Clostridium] symbiosum | |
| beta-gamma-methylene ATP | competitive to ATP | Propionibacterium freudenreichii subsp. shermanii | |
| Bromopyruvate | competitive to phosphoenolpyruvate | [Clostridium] symbiosum | |
| Bromopyruvate | competitive to phosphoenolpyruvate | Propionibacterium freudenreichii subsp. shermanii | |
| Ca2+ | - |
[Clostridium] symbiosum | |
| Ca2+ | competitive to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
| CTP | - |
[Clostridium] symbiosum | |
| CTP | - |
Propionibacterium freudenreichii subsp. shermanii | |
| dATP | - |
[Clostridium] symbiosum | |
| dATP | - |
Propionibacterium freudenreichii subsp. shermanii | |
| Fe2+ | competitive to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
| GMP | competitive to ATP | Propionibacterium freudenreichii subsp. shermanii | |
| GTP | competitive to ATP | Propionibacterium freudenreichii subsp. shermanii | |
| iodoacetate | - |
[Clostridium] symbiosum | |
| iodoacetate | - |
Propionibacterium freudenreichii subsp. shermanii | |
| ITP | - |
[Clostridium] symbiosum | |
| ITP | - |
Propionibacterium freudenreichii subsp. shermanii | |
| Methylene diphosphonate | competitive to diphosphate | [Clostridium] symbiosum | |
| Methylene diphosphonate | competitive to diphosphate | Propionibacterium freudenreichii subsp. shermanii | |
| additional information | not inhibited by iodoacetamide | [Clostridium] symbiosum | |
| additional information | not inhibited by iodoacetamide | Propionibacterium freudenreichii subsp. shermanii | |
| N-ethylmaleimide | - |
[Clostridium] symbiosum | |
| N-ethylmaleimide | - |
Propionibacterium freudenreichii subsp. shermanii | |
| p-hydroxymercuribenzoate | - |
[Clostridium] symbiosum | |
| p-hydroxymercuribenzoate | - |
Propionibacterium freudenreichii subsp. shermanii | |
| Phosphoglycolate | competitive to phosphoenolpyruvate | [Clostridium] symbiosum | |
| Sulfhydryl agents | - |
[Clostridium] symbiosum | |
| Sulfhydryl agents | - |
Propionibacterium freudenreichii subsp. shermanii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
AMP | pH 6.8, 25ºC | Propionibacterium freudenreichii subsp. shermanii | |
| 0.004 | - |
ATP | pH 6.8, 25ºC | Propionibacterium freudenreichii subsp. shermanii | |
| 0.015 | - |
AMP | pH 6.7, 25ºC | [Clostridium] symbiosum | |
| 0.05 | - |
ATP | pH 6.7, 25ºC | [Clostridium] symbiosum | |
| 0.08 | - |
diphosphate | pH 6.7, 25ºC | [Clostridium] symbiosum | |
| 0.1 | - |
pyruvate | pH 6.8, 25ºC | Propionibacterium freudenreichii subsp. shermanii | |
| 0.1 | - |
pyruvate | pH 6.7, 25ºC | [Clostridium] symbiosum | |
| 0.1 | - |
phosphoenolpyruvate | pH 6.7, 25ºC | [Clostridium] symbiosum | |
| 0.8 | - |
phosphate | pH 6.7, 25ºC | [Clostridium] symbiosum | |
| 1 | - |
phosphate | pH 6.8, 25ºC | Propionibacterium freudenreichii subsp. shermanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
[Clostridium] symbiosum | |
| K+ | - |
[Clostridium] symbiosum | |
| Mg2+ | - |
[Clostridium] symbiosum | |
| Mn2+ | - |
[Clostridium] symbiosum | |
| NH4+ | - |
[Clostridium] symbiosum | |
| Rb+ | 25% of the activity relative to NH4+ | [Clostridium] symbiosum | |
| Tl+ | 94% of the activity relative to NH4+ | [Clostridium] symbiosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
2 * 75000, SDS-PAGE | [Clostridium] symbiosum |
| 150000 | - |
gel filtration | Propionibacterium freudenreichii subsp. shermanii |
| 150000 | 160000 | equilibrium sedimentation and gel filtration | [Clostridium] symbiosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
| [Clostridium] symbiosum | - |
ATCC 14940 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using ammonium sulfate precipitation and chromatography on DEAE-cellulose and Sephadex G-200 | Propionibacterium freudenreichii subsp. shermanii |
| partial, using ammonium sulfate precipitation, and chromatography on DEAE-cellulose and Bio-Gel | [Clostridium] symbiosum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate | mechanism | Propionibacterium freudenreichii subsp. shermanii | |
| ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate | mechanism | [Clostridium] symbiosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.1 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
| 35 | - |
- |
[Clostridium] symbiosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + phosphate | specificity is strictly restricted to adenine nucleotides | Propionibacterium freudenreichii subsp. shermanii | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
| ATP + pyruvate + phosphate | specificity is strictly restricted to adenine nucleotides | [Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
| ATP + pyruvate + phosphate | dAMP can replace ATP but with 20% of the activity | [Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 75000, SDS-PAGE | [Clostridium] symbiosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Propionibacterium freudenreichii subsp. shermanii |
| 25 | - |
assay at | [Clostridium] symbiosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 6.7 | pyruvate formation | [Clostridium] symbiosum |
| 6.5 | 7.5 | phosphoenolpyruvate formation | Propionibacterium freudenreichii subsp. shermanii |
| 6.8 | - |
pyruvate formation | Propionibacterium freudenreichii subsp. shermanii |
| 7 | 7.8 | phosphoenolpyruvate formation | [Clostridium] symbiosum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 6.7 | - |
Propionibacterium freudenreichii subsp. shermanii |
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