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Literature summary for 2.7.9.1 extracted from
- Pyruvate, orthophosphate dikinase from Acetobacter xylinum (1975), Methods Enzymol., 42C, 192-199.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | phosphoenolpyruvate formation, competitive to ATP | Komagataeibacter xylinus |  |
| ATP | pyruvate formation, competitive to AMP | Komagataeibacter xylinus | |
| p-hydroxymercuribenzoate | - |
Komagataeibacter xylinus | |
| potassium fluoride | inhibits reaction in both directions at 50 mM | Komagataeibacter xylinus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
AMP | pH 6.5, 30ºC, pyruvate formation | Komagataeibacter xylinus | |
| 0.06 | - |
diphosphate | pH 6.5, 30ºC, pyruvate formation | Komagataeibacter xylinus | |
| 0.1 | - |
phosphoenolpyruvate | pH 6.5, 30ºC, pyruvate formation | Komagataeibacter xylinus | |
| 0.2 | - |
pyruvate | pH 8.2, 30ºC, phosphoenolpyruvate formation | Komagataeibacter xylinus | |
| 0.4 | - |
ATP | pH 8.2, 30ºC, phosphoenolpyruvate formation | Komagataeibacter xylinus | |
| 0.8 | - |
phosphate | pH 8.2, 30ºC, phosphoenolpyruvate formation | Komagataeibacter xylinus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Komagataeibacter xylinus | |
| Mn2+ | cannot replace Mg2+ in either the forward or the reverse reaction | Komagataeibacter xylinus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + phosphate | Komagataeibacter xylinus | enzyme activity has implication in the regulation of gluconeogenesis and carbohydrate oxidation | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Komagataeibacter xylinus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using ammonium sulfate precipitation and chromatography on DEAE-cellulose | Komagataeibacter xylinus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.88 | - |
- |
Komagataeibacter xylinus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| retains 85% of activity after two weeks at room temperature | Komagataeibacter xylinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + arsenate | the rate of phosphoenolpyruvate formation in the presence of arsenate is 65% lower than that obtained with phosphate | Komagataeibacter xylinus | ? | - |
? | |
| ATP + pyruvate + phosphate | GTP, CTP, ITP or TTP cannot replace ATP in the reaction with pyruvate | Komagataeibacter xylinus | AMP + phosphoenolpyruvate + diphosphate | GDP, CMP and ADP cannot replace AMP in the reverse reaction | r | |
| ATP + pyruvate + phosphate | enzyme activity has implication in the regulation of gluconeogenesis and carbohydrate oxidation | Komagataeibacter xylinus | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Komagataeibacter xylinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
pyruvate formation | Komagataeibacter xylinus |
| 8.2 | - |
phosphoenolpyruvate formation | Komagataeibacter xylinus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
AMP | pH 8.2, 30ºC, phosphoenolpyruvate formation | Komagataeibacter xylinus | |
| 0.22 | - |
ATP | pH 6.5, 30ºC, pyruvate formation | Komagataeibacter xylinus | |
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