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Literature summary for 2.7.9.1 extracted from
- Pyruvate,phosphate dikinase from Bacteroides symbiosus (1971), Biochem. J., 125, 531-539.
General Stability
| General Stability | Organism |
|---|---|
| does not require thiol compounds to maintain stability during storage or assay | [Clostridium] symbiosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CTP | total inhibition of the forward reaction at 1 mM | [Clostridium] symbiosum |  |
| dATP | 40% inhibition at 1 mM | [Clostridium] symbiosum | |
| ITP | total inhibition of the forward reaction at 1 mM | [Clostridium] symbiosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
AMP | pH 6.8, 25ºC, phosphoenolpyruvate formation | [Clostridium] symbiosum | |
| 0.08 | - |
pyruvate | pH 6.8, 25ºC, pyruvate formation | [Clostridium] symbiosum | |
| 0.1 | - |
ATP | pH 6.8, 25ºC, pyruvate formation | [Clostridium] symbiosum | |
| 0.6 | - |
phosphate | pH 6.8, 25ºC, pyruvate formation | [Clostridium] symbiosum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM of this ion promotes pyruvate formation | [Clostridium] symbiosum | |
| K+ | half-maximal activity with 20 mM potassium chloride at pH 6.8 | [Clostridium] symbiosum | |
| Mg2+ | 1 mM of this ion promotes reaction in both directions | [Clostridium] symbiosum | |
| Mn2+ | 1 mM of this ion promotes pyruvate formation | [Clostridium] symbiosum | |
| NH4+ | - |
[Clostridium] symbiosum | |
| Ni2+ | 1 mM of this ion promotes pyruvate formation | [Clostridium] symbiosum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Clostridium] symbiosum | - |
ATCC 14940 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using ammonium sulfate precipitation, and chromatography on DEAE-cellulose and Bio-Gel | [Clostridium] symbiosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
[Clostridium] symbiosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + phosphate | specificity is strictly restricted to adenine nucleotides | [Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | [Clostridium] symbiosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
reverse reaction, pyruvate formation | [Clostridium] symbiosum |
| 7.2 | 7.8 | forward reaction, phosphoenolpyruvate formation | [Clostridium] symbiosum |
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Release 2023.1 (January 2023)
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