Cloned (Comment) | Organism |
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- |
Escherichia coli |
Protein Variants | Comment | Organism |
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additional information | Escherichia coli has three cardiolipin synthases. Cardiolipin synthase A encoded by clsA, cardiolipin synthase B encoded by clsB and cardiolipin synthase C, encoded by clsC. Deletion of all three genes (DELTAclsABC) results in a complete lack of cardiolipin. Restoration of nearwild-type levels of cardiolipin production in the stationary phase by expression of clsC in the triple mutant requires the coexpression from the same operon of the preceding gene ymdB. The mode of cardiolipin biosynthesis by the combined YmdB-ClsC follows neither the eukaryotic nor prokaryotic pathway but involves the transfer of a phosphatidyl moiety from PE to the terminal free hydroxyl of PG to form cardiolipin | Escherichia coli |
Organism | UniProt | Comment | Textmining |
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Escherichia coli | P75919 | cardiolipin synthase C | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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1-(1-heptadecanoyl-2-((9Z)-tetradec-9-enoyl)-sn-glycero-3-phospho)-sn-glycerol + 1-dodecanoyl-2-tridecanoyl-sn-glycero-3-phosphoethanolamine | synthetic phosphatidylglycerol (17:0/14:1) and synthetic phosphatidylethanolamine (12:0/13:0). Reaction is catalyzed by the two-component complex YmdB-ClsC. Escherichia coli has three cardiolipin synthases. Cardiolipin synthase A encoded by clsA, cardiolipin synthase B encoded by clsB and cardiolipin synthase C, encoded by clsC. Deletion of all three genes (DELTAclsABC) results in a complete lack of cardiolipin. Restoration of nearwild-type levels of cardiolipin production in the stationary phase by expression of clsC in the triple mutant requires the coexpression from the same operon of the preceding gene ymdB. The mode of cardiolipin biosynthesis by the combined YmdB-ClsC follows neither the eukaryotic nor prokaryotic pathway but involves the transfer of a phosphatidyl moiety from phosphatidylethanolamine to the terminal free hydroxyl of phosphatidylglycerol to form cardiolipin | Escherichia coli | 1-(1-heptadecanoyl-2-((9Z)-tetradec-9-enoyl)-sn-glycero-3-phospho)-3-(1-dodecanoyl-2-tridecanoyl-sn-glycero-3-phospho)glycerol + ethanolamine | - |
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Subunits | Comment | Organism |
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More | reaction is catalyzed by the two-component complex YmdB-ClsC | Escherichia coli |
Synonyms | Comment | Organism |
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clsC | - |
Escherichia coli |
General Information | Comment | Organism |
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malfunction | Escherichia coli has three cardiolipin synthases. Cardiolipin synthase A encoded by clsA, cardiolipin synthase B encoded by clsB and cardiolipin synthase C, encoded by clsC. Triple deletions of clsA, clsB, and clsC results in the complete depletion of cardiolipin synthase activity in Escherichia coli cells. The double mutant DELTAclsAB mutant still makes cardiolipin in the stationary phase | Escherichia coli |
physiological function | Escherichia coli has three cardiolipin synthases. All cardiolipin synthases contribute cardiolipin the stationary phase. Only cardiolipin synthase A makes detectible cardiolipin in low-osmolarity medium during logarithmic growth and contributes the majority of cardiolipin in high-osmolarity medium | Escherichia coli |