Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, recombinant expression of tagged isozyme in Escherichia coli | Streptomyces tsukubensis |
gene acpS, DNA and amino acid sequence determination and analysis, recombinant expression of tagged isozyme in Escherichia coli | Streptomyces tsukubensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Streptomyces tsukubensis | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Streptomyces tsukubensis L19 | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces tsukubensis | A0A0X8EK72 | isolated from Yunnan, China | - |
Streptomyces tsukubensis | A0A0Y0S258 | isolated from Yunnan, China | - |
Streptomyces tsukubensis | A0A0Y0SAB4 | isolated from Yunnan, China | - |
Streptomyces tsukubensis | A0A109R3N8 | isolated from Yunnan, China | - |
Streptomyces tsukubensis | I2N4F3 | isolated from Yunnan, China | - |
Streptomyces tsukubensis L19 | A0A0X8EK72 | isolated from Yunnan, China | - |
Streptomyces tsukubensis L19 | A0A0Y0S258 | isolated from Yunnan, China | - |
Streptomyces tsukubensis L19 | A0A0Y0SAB4 | isolated from Yunnan, China | - |
Streptomyces tsukubensis L19 | A0A109R3N8 | isolated from Yunnan, China | - |
Streptomyces tsukubensis L19 | I2N4F3 | isolated from Yunnan, China | - |
Purification (Comment) | Organism |
---|---|
recombinant tagged isozyme from Escherichia coli by affinity chromatography | Streptomyces tsukubensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Streptomyces tsukubensis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Streptomyces tsukubensis L19 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
additional information | substrate specificities of isozymes with different ACPs: stw ACP, an ACP in a type II PKS, is phosphopantetheinylated by three PPTases FKPPT1, FKPPT3, and FKACPS. sts FAS ACP, the ACP in fatty acid synthase (FAS), is phosphopantetheinylated by three PPTases FKPPT2, FKPPT3, and FKACPS. TcsA-ACP, an ACP involved in FK506 biosynthesis, is phosphopantetheinylated by two PPTases FKPPT3 and FKACPS. FkbPPCP, an PCP involved in FK506 biosynthesis, is phosphopantetheinylated by all of these five PPTases FKPPT1-4 and FKACPS | Streptomyces tsukubensis | ? | - |
? | |
additional information | substrate specificities of isozymes with different ACPs: stw ACP, an ACP in a type II PKS, is phosphopantetheinylated by three PPTases FKPPT1, FKPPT3, and FKACPS. sts FAS ACP, the ACP in fatty acid synthase (FAS), is phosphopantetheinylated by three PPTases FKPPT2, FKPPT3, and FKACPS. TcsA-ACP, an ACP involved in FK506 biosynthesis, is phosphopantetheinylated by two PPTases FKPPT3 and FKACPS. FkbPPCP, an PCP involved in FK506 biosynthesis, is phosphopantetheinylated by all of these five PPTases FKPPT1-4 and FKACPS | Streptomyces tsukubensis L19 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FKACPS | - |
Streptomyces tsukubensis |
FKPPT1 | - |
Streptomyces tsukubensis |
FKPPT2 | - |
Streptomyces tsukubensis |
FKPPT3 | - |
Streptomyces tsukubensis |
FKPPT4 | - |
Streptomyces tsukubensis |
phosphopantetheinyl transferase | - |
Streptomyces tsukubensis |
PPTase | - |
Streptomyces tsukubensis |
General Information | Comment | Organism |
---|---|---|
evolution | PPTases can be classified into three groups according to their structures. A group I PPTase, also named as an ACPS-type PPTase, consists of three identical peptide subunits with about 120 amino acid residues in each subunit. A group II PPTase, also named as a Sfp-type PPTase, consists of one peptide which is about twice the size of one group I PPTase subunit. A group III PPTase exists as a domain of a FAS or a PKS. FKPPT1, FKPPT2, FKPPT3, FKPPT4 contains three conserved motifs, PRWP, GID and FSAKESVYK, found in the Sfp-type PPTase motifs P1, P2 and P3, while FKACPS contains just last two conserved motifs found in ACPS-type PPTase. Thus, FKPPT1, FKPPT2, FKPPT3, and FKPPT4 belong to the Sfp-type PPTase group, and FKACPS belongs to the ACPS-type PPTase group, respectively | Streptomyces tsukubensis |
malfunction | inactivation of FKPPT1 or FKPPT3 decreases the FK506 yield in Streptomyces tsukubaensis strain L19 | Streptomyces tsukubensis |
malfunction | inactivation of FKPPT1 or FKPPT3 decreases the FK506 yield in Streptomyces tsukubaensis strain L19, and overexpression of FKPPT3 increases the FK506 production | Streptomyces tsukubensis |
physiological function | phosphopantetheinyl transferases (PPTases) catalyze the phosphopantetheinylation of acyl carrier proteins (ACPs) in polyketide synthases (PKSs), peptidyl carrier proteins (PCPs) in nonribosomal peptide synthetases (NRPSs), and ACPs in fatty acid synthases (FASs) from inactive apo-forms into active holo-form. PPTases are essential to both primary metabolisms and secondary metabolisms. Phosphopantetheinylation of the ACP/PCP is involved in FK506 biosynthesis, FK506 (tacrolimus) is a clinical immunosuppressant widely used after allogeneic kidney, liver, and heart transplantations | Streptomyces tsukubensis |