Literature summary for 2.7.8.7 extracted from

Rottier, K.; Faille, A.; Prudhomme, T.; Leblanc, C.; Chalut, C.; Cabantous, S.; Guilhot, C.; Mourey, L.; Pedelacq, J.D.
Detection of soluble co-factor dependent protein expression in vivo: application to the 4'-phosphopantetheinyl transferase PptT from Mycobacterium tuberculosis (2013), J. Struct. Biol., 183, 320-328.

Cloned(Commentary)

Cloned (Comment)	Organism
gene pptT, recombinant expression of His-tagged wild-type full-length PptT and N- and C-terminally truncated mutant enzymes in Escherichia coli strain BL21(DE3), the wild-type full-lebgth enzyme forms aggregates, expression of the enzyme with a ubiquitin-related modifier tag (SUMO) has no effect on the protein solubility, while fusion with the maltose-binding-protein results in a soluble fusion protein that can be purified	Mycobacterium tuberculosis

Cloned (Comment)

Organism

gene pptT, recombinant expression of His-tagged wild-type full-length PptT and N- and C-terminally truncated mutant enzymes in Escherichia coli strain BL21(DE3), the wild-type full-lebgth enzyme forms aggregates, expression of the enzyme with a ubiquitin-related modifier tag (SUMO) has no effect on the protein solubility, while fusion with the maltose-binding-protein results in a soluble fusion protein that can be purified

Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
additional information	improved solubility of the full-length PptT compared to its N- and C-terminally truncated counterparts. In vivo, the highest fluorescence levels is attained for the full-length enzyme and N-terminally truncated variants whereas truncation of the last C-terminal residue L227 is sufficient to negatively impact the fluorescence intensity of the colonies	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism
Mg2+	dependent on	Mycobacterium tuberculosis
Mn2+	activates	Mycobacterium tuberculosis
additional information	Mg2+ results in the highest activity among the divalent ions tested followed by Mn2+, while Ca2+ has no effect on the activity of the enzyme	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]	Mycobacterium tuberculosis	-	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]	Mycobacterium tuberculosis ATCC 25618	-	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	O33336	-	-
Mycobacterium tuberculosis ATCC 25618	O33336	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type full-length PptT and N- and C-terminally truncated mutant enzymes from Escherichia coli strain BL21(DE3) and recombinant soluble MBP-fusion PptT by immobilized metal ion affinity chromatography and gel filtration. The MBP-tagged enzyme irreversibly precipitates after proteolytic cleavage of the N-terminal MBP tag	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]	-	Mycobacterium tuberculosis	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]	with the most soluble and compact ACP fragment (2042-2188) of ACP substrate from the type I polyketide synthase PpsC from Mycobacterium tuberculosis	Mycobacterium tuberculosis	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]	-	Mycobacterium tuberculosis ATCC 25618	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]	with the most soluble and compact ACP fragment (2042-2188) of ACP substrate from the type I polyketide synthase PpsC from Mycobacterium tuberculosis	Mycobacterium tuberculosis ATCC 25618	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	?

Synonyms

Synonyms	Comment	Organism
4'-phosphopantetheinyl transferase	-	Mycobacterium tuberculosis
PptT	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Mycobacterium tuberculosis

General Information

General Information	Comment	Organism
additional information	improved solubility of the full-length PptT compared to its N- and C-terminally truncated counterparts	Mycobacterium tuberculosis
physiological function	the enzyme is essential for the viability of the bacterium	Mycobacterium tuberculosis