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Literature summary for 2.7.8.7 extracted from

  • Owen, J.G.; Copp, J.N.; Ackerley, D.F.
    Rapid and flexible biochemical assays for evaluating 4-phosphopantetheinyl transferase activity (2011), Biochem. J., 436, 709-717.
    View publication on PubMed
Search for more literature for 2.7.8.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis
expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
6-nitroso-1,2-benzopyrone
-
 Bacillus subtilis
6-nitroso-1,2-benzopyrone
-
 Pseudomonas aeruginosa
6-nitroso-1,2-benzopyrone
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00062
-
 CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
0.0033
-
 apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
0.0038
-
 CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
0.0065
-
 apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
0.0086
-
 apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
0.011
-
 CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Bacillus subtilis
Mg2+ required for activity Pseudomonas aeruginosa
Mg2+ required for activity Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis ATCC 6633
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas putida
-
-
-
Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus subtilis
Ni-NTA column chromatography Pseudomonas aeruginosa
Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
-
 Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[BpsA protein]
-
 ?
CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
-
 Pseudomonas aeruginosa adenosine 3',5'-bisphosphate + holo-[BpsA protein]
-
 ?
CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
-
 Pseudomonas putida adenosine 3',5'-bisphosphate + holo-[BpsA protein]
-
 ?
CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
-
 Pseudomonas putida KT 2240 adenosine 3',5'-bisphosphate + holo-[BpsA protein]
-
 ?
CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
-
 Bacillus subtilis ATCC 6633 adenosine 3',5'-bisphosphate + holo-[BpsA protein]
-
 ?

Synonyms

Synonyms Comment Organism
4-phosphopantetheinyl transferase
-
 Bacillus subtilis
4-phosphopantetheinyl transferase
-
 Pseudomonas aeruginosa
4-phosphopantetheinyl transferase
-
 Pseudomonas putida
PcpS
-
 Pseudomonas aeruginosa
PP1183
-
 Pseudomonas putida
PPTase
-
 Bacillus subtilis
PPTase
-
 Pseudomonas aeruginosa
PPTase
-
 Pseudomonas putida
Sfp
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0027
-
 CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
0.035
-
 apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
0.037
-
 CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
0.075
-
 CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
0.183
-
 apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
0.683
-
 apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
4'-phosphopantetheine
-
 Bacillus subtilis
4'-phosphopantetheine
-
 Pseudomonas aeruginosa
4'-phosphopantetheine
-
 Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00042
-
 6-nitroso-1,2-benzopyrone in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
0.00053
-
 6-nitroso-1,2-benzopyrone in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
0.0024
-
 6-nitroso-1,2-benzopyrone in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
0.003
-
 6-nitroso-1,2-benzopyrone in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
0.003
-
 6-nitroso-1,2-benzopyrone in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
0.0052
-
 6-nitroso-1,2-benzopyrone in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0021
-
 in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis 6-nitroso-1,2-benzopyrone
0.0037
-
 in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida 6-nitroso-1,2-benzopyrone
0.004
-
 in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa 6-nitroso-1,2-benzopyrone
0.0091
-
 in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis 6-nitroso-1,2-benzopyrone
0.0098
-
 in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida 6-nitroso-1,2-benzopyrone
0.0108
-
 in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa 6-nitroso-1,2-benzopyrone

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.3
-
 CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
3.5
-
 CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
20
-
 CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
21.7
-
 apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
107
-
 apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
108
-
 apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis