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Literature summary for 2.7.8.7 extracted from

  • Sanchez, C.; Du, L.; Edwards, D.J.; Toney, M.D.; Shen, B.
    Cloning and characterization of a phosphopantetheinyl transferase from Streptomyces verticillus ATCC15003, the producer of the hybrid peptide-polyketide antitumor drug bleomycin (2001), Chem. Biol., 8, 725-738.
    View publication on PubMed

Application

Application Comment Organism
medicine producer of the hybrid peptide-polyketide antitumor drug bleomycin Streptomyces verticillus

Cloned(Commentary)

Cloned (Comment) Organism
svp gene cloned into pQE-70 and overproduced in Escherichia coli M15(pREP4) Streptomyces verticillus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
apo-[peptidyl carrier protein] pH 8.0, 37°C Streptomyces verticillus
0.0039
-
apo-[peptidyl carrier protein] pH 8.0, 37°C Streptomyces verticillus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26830
-
calculated from amino acid sequence of His6-tagged svp Streptomyces verticillus
31000
-
1 * 31000, SDS-PAGE Streptomyces verticillus
31600
-
gel filtration Streptomyces verticillus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + apo-[acyl-carrier protein] Streptomyces verticillus
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r
CoA + apo-[acyl-carrier protein] Streptomyces verticillus ATCC 15003
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r

Organism

Organism UniProt Comment Textmining
Streptomyces verticillus Q9F0Q6 strain ATCC15003, nucleotide sequence of the svp locus
-
Streptomyces verticillus ATCC 15003 Q9F0Q6 strain ATCC15003, nucleotide sequence of the svp locus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expressed in Escherichia coli Streptomyces verticillus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + apo-[acyl-carrier protein]
-
Streptomyces verticillus adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r
CoA + apo-[acyl-carrier protein]
-
Streptomyces verticillus ATCC 15003 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r
CoA + apo-[peptidyl carrier protein]
-
Streptomyces verticillus adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
r
CoA + apo-[peptidyl carrier protein]
-
Streptomyces verticillus ATCC 15003 adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
r
additional information posttranslational modification of carrier proteins, capable of modifying both type I and type II acyl carrier proteins and peptidyl carrier proteins, even form other Streptomyces sp. Streptomyces verticillus ?
-
?
additional information posttranslational modification of carrier proteins, capable of modifying both type I and type II acyl carrier proteins and peptidyl carrier proteins, even form other Streptomyces sp. Streptomyces verticillus ATCC 15003 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Streptomyces verticillus

Synonyms

Synonyms Comment Organism
PPTase
-
Streptomyces verticillus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.183
-
apo-[peptidyl carrier protein] pH 8.0, 37°C, substrate BlmI Streptomyces verticillus
1.43
-
apo-[peptidyl carrier protein] pH 8.0, 37°C, substrate TcmM Streptomyces verticillus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Streptomyces verticillus