Application | Comment | Organism |
---|---|---|
medicine | producer of the hybrid peptide-polyketide antitumor drug bleomycin | Streptomyces verticillus |
Cloned (Comment) | Organism |
---|---|
svp gene cloned into pQE-70 and overproduced in Escherichia coli M15(pREP4) | Streptomyces verticillus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
apo-[peptidyl carrier protein] | pH 8.0, 37°C | Streptomyces verticillus | |
0.0039 | - |
apo-[peptidyl carrier protein] | pH 8.0, 37°C | Streptomyces verticillus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26830 | - |
calculated from amino acid sequence of His6-tagged svp | Streptomyces verticillus |
31000 | - |
1 * 31000, SDS-PAGE | Streptomyces verticillus |
31600 | - |
gel filtration | Streptomyces verticillus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | Streptomyces verticillus | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | Streptomyces verticillus ATCC 15003 | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces verticillus | Q9F0Q6 | strain ATCC15003, nucleotide sequence of the svp locus | - |
Streptomyces verticillus ATCC 15003 | Q9F0Q6 | strain ATCC15003, nucleotide sequence of the svp locus | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, expressed in Escherichia coli | Streptomyces verticillus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | - |
Streptomyces verticillus | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | - |
Streptomyces verticillus ATCC 15003 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[peptidyl carrier protein] | - |
Streptomyces verticillus | adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein] | - |
r | |
CoA + apo-[peptidyl carrier protein] | - |
Streptomyces verticillus ATCC 15003 | adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein] | - |
r | |
additional information | posttranslational modification of carrier proteins, capable of modifying both type I and type II acyl carrier proteins and peptidyl carrier proteins, even form other Streptomyces sp. | Streptomyces verticillus | ? | - |
? | |
additional information | posttranslational modification of carrier proteins, capable of modifying both type I and type II acyl carrier proteins and peptidyl carrier proteins, even form other Streptomyces sp. | Streptomyces verticillus ATCC 15003 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000, SDS-PAGE | Streptomyces verticillus |
Synonyms | Comment | Organism |
---|---|---|
PPTase | - |
Streptomyces verticillus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.183 | - |
apo-[peptidyl carrier protein] | pH 8.0, 37°C, substrate BlmI | Streptomyces verticillus | |
1.43 | - |
apo-[peptidyl carrier protein] | pH 8.0, 37°C, substrate TcmM | Streptomyces verticillus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Streptomyces verticillus |