Literature summary for 2.7.8.7 extracted from

Gehring, A.M.; Lambalot, R.H.; Vogel, K.W.; Drueckhammer, D.G.; Walsh, C.T.
Ability of Streptomyces spp. acyl carrier proteins and coenzyme A analogs to serve as substrates in vitro for E. coli holo-ACP synthase (1997), Chem. Biol., 4, 17-24.

Search for more literature for 2.7.8.7

Inhibitors

Inhibitors	Comment	Organism	Structure
apo-acyl-carrier protein	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.002	-	apo-acyl-carrier protein	pH 7.0, 37°C, Escherichia coli acyl carrier protein	Escherichia coli
0.005	-	apo-[Streptomyces sp. granaticin-acyl-carrier protein]	pH 7.0, 37°C, Escherichia coli acyl carrier protein	Escherichia coli
0.012	-	apo-[Streptomyces sp. frenolicin-acyl-carrier protein]	pH 7.0, 37°C, Escherichia coli acyl carrier protein	Escherichia coli
0.022	-	apo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]	pH 7.0, 37°C, Escherichia coli acyl carrier protein	Escherichia coli
0.039	-	apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]	pH 7.0, 37°C, Escherichia coli acyl carrier protein	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CoA + apo-[acyl-carrier protein]	Escherichia coli	-	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetonyldethio-CoA + apo-[acyl-carrier protein]	-	Escherichia coli	? + holo-[acyl-carrier protein]	-	r
CoA + apo-[acyl-carrier protein]	-	Escherichia coli	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	r
CoA + apo-[acyl-carrier protein]	Streptomycess sp. acyl carrier proteins and coenzyme A analogs also serves as substrates for holo-ACP synthase in vitro	Escherichia coli	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	r
CoA + apo-[Streptomyces sp. frenolicin-acyl-carrier protein]	-	Escherichia coli	adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. frenolicin-acyl-carrier protein]	-	r
CoA + apo-[Streptomyces sp. granaticin-acyl-carrier protein]	-	Escherichia coli	adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. granaticin-acyl-carrier protein]	-	r
CoA + apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]	-	Escherichia coli	adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]	-	r
CoA + apo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]	-	Escherichia coli	adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]	-	r
desulfo-CoA + apo-[acyl-carrier protein]	-	Escherichia coli	? + holo-[acyl-carrier protein]	-	r
desulfoCoA + apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]	-	Escherichia coli	? + holo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]	-	r
homocysteamine-CoA + apo-[acyl-carrier protein]	-	Escherichia coli	? + holo-[acyl-carrier protein]	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.09	-	apo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]	pH 7.0, 37°C, E. coli ACP	Escherichia coli
0.167	-	apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]	pH 7.0, 37°C, E. coli ACP	Escherichia coli
0.317	-	apo-[Streptomyces sp. frenolicin-acyl-carrier protein]	pH 7.0, 37°C, E. coli ACP	Escherichia coli
0.5	-	apo-[Streptomyces sp. granaticin-acyl-carrier protein]	pH 7.0, 37°C, E. coli ACP	Escherichia coli
1.33	1.67	apo-acyl-carrier protein	pH 7.0, 37°C, E. coli ACP	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0006	-	apo-[Streptomyces sp. granaticin-acyl-carrier protein]	pH 7.0, 37°C, E. coli ACP	Escherichia coli
0.002	-	apo-acyl-carrier protein	pH 7.0, 37°C, E. coli ACP	Escherichia coli
0.054	-	apo-[Streptomyces sp. frenolicin-acyl-carrier protein]	pH 7.0, 37°C, E. coli ACP	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)