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Literature summary for 2.7.8.6 extracted from

  • Patel, K.; Ciepichal, E.; Swiezewska, E.; Valvano, M.
    The C-terminal domain of the Salmonella enterica WbaP (UDP-galactose:Und-P galactose-1-phosphate transferase) is sufficient for catalytic activity and specificity for undecaprenyl monophosphate (2012), Glycobiology, 22, 116-122.
    View publication on PubMed
Search for more literature for 2.7.8.6

Cloned(Commentary)

Cloned (Comment) Organism
the His6-tagged catalytic domain is expressed in Escherichia coli BL21(DE3) and C43(DE3) cells Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
CHAPS 62.4% relative activity at 0.5 mM Salmonella enterica
KCl KCl inhibits the enzyme domain activity, particularly at concentrations higher than 200 mM salt that result in more than 50% inhibition Salmonella enterica
Mg2+ no activity at 150 mM Salmonella enterica
n-dodecyl-beta-D-maltoside 81.5% relative activity at 0.5 mM Salmonella enterica
NaCl NaCl inhibits the enzyme domain activity, particularly at concentrations higher than 200 mM salt that result in more than 50% inhibition Salmonella enterica
octylglucoside 65.5% relative activity at 0.5 mM Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00055
-
 UDP-alpha-D-galactose in 50 mM Tris-HCl (pH 8.5), 25 mM MgCl2, 1% (w/v) CHAPS, at 37°C Salmonella enterica
0.0785
-
 undecaprenyl phosphate in 50 mM Tris-HCl (pH 8.5), 25 mM MgCl2, 1% (w/v) CHAPS, at 37°C Salmonella enterica

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Salmonella enterica 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ without adding MgCl2, no activity can be detected. Activity is optimal at 25 mM Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, His6-tagged catalytic domain, SDS-PAGE Salmonella enterica

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-100 3.5% relative activity at 1 mM Salmonella enterica

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-bound chelating Sepharose column chromatography, and gel filtration Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information minimal activity is detected with the shorter polyisoprenyl phosphates like C10-P, C15-P, C20-P and C35-P at 0.28, 0.91, 1 and 1.8% of C55-undecaprenyl phosphate activity, respectively. C50-phosphate, however, results in 49.53% activity of C55-phosphate Salmonella enterica ?
-
 ?
UDP-alpha-D-galactose + undecaprenyl phosphate specific activity with C55-undecaprenyl phosphate Salmonella enterica UMP + alpha-D-galactosyl-diphosphoundecaprenol
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, His6-tagged catalytic domain, SDS-PAGE Salmonella enterica

Synonyms

Synonyms Comment Organism
UDP-galactose:Und-P galactose-1-phosphate transferase
-
 Salmonella enterica
WbaP
-
 Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Salmonella enterica

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
 Salmonella enterica