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Literature summary for 2.7.8.5 extracted from

  • Hirabayashi, T.; Larson, T.J.; Dowhan, W.
    Membrane-associated phosphatidylglycerophosphate synthetase from Escherichia coli: purification by substrate affinity chromatography on cytidine 5-diphospho-1,2-diacyl-sn-glycerol sepharose (1976), Biochemistry, 15, 5205-5211.
    View publication on PubMed
Search for more literature for 2.7.8.5

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 0.5-6%, absolute requirement Escherichia coli

General Stability

General Stability Organism
combination of both 4 M urea and 1% SDS at 30°C completely inactivates Escherichia coli
SDS, 1%, 50% loss of activity after 2 h at 30°C Escherichia coli
urea, 8 M, stable in presence of 0.1% Triton X-100 after 2 h at 30°C Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
CDP-diacylglycerol inhibition of CDP-diacylglycerol formation Escherichia coli
glycerol 3-phosphate inhibition of CDP-diacylglycerol formation Escherichia coli
Liponucleotide forms a dead-end complex at high concentrations inhibiting both, the forward and the reverse reaction Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 dCDP-diacylglycerol 30°C, pH 8.0 Escherichia coli
0.046
-
 CDP-diacylglycerol 30°C, pH 8.0 Escherichia coli
0.32
-
 sn-glycerol 3-phosphate 30°C, pH 8.0 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, SDS-PAGE   Escherichia coli
200000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 1-(3-sn-phosphatidyl)-sn-glycerol 3-phosphate ordered sequential bi-bi reaction Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-diacylglycerol + sn-glycero-3-phosphate
-
 Escherichia coli CMP + phosphatidylglycerophosphate 3-sn-phosphatidyl-1'-sn-glycerol 3'-phosphate r
dCDP-diacylglycerol + sn-glycero-3-phosphate
-
 Escherichia coli dCMP + phosphatidylglycerophosphate
-
 ?
additional information cytidine 5'-monophosphate dependent exchange between glycerol 3-phosphate and phosphatidylglycerophosphate Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE   Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 5 min, stable, partially purified enzyme, Triton X-100 extract Escherichia coli
60
-
 5 min, 50% loss of activity, partially purified enzyme, Triton X-100 extract Escherichia coli
65
-
 5 min, complete loss of activity, partially purified enzyme, Triton X-100 extract Escherichia coli