Literature summary for 2.7.8.44 extracted from

Bhavsar, A.P.; Truant, R.; Brown, E.D.
The TagB protein in Bacillus subtilis 168 is an intracellular peripheral membrane protein that can incorporate glycerol phosphate onto a membrane-bound acceptor in vitro (2005), J. Biol. Chem., 280, 36691-36700.

Search for more literature for 2.7.8.44

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
H126A	the mutant shows 75% less activity relative to the wild type enzyme	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasmic membrane	-	Bacillus subtilis	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47079	-	-	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol	Bacillus subtilis	-	CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol	-	?
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol	Bacillus subtilis 168	-	CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P27621	-	-
Bacillus subtilis 168	P27621	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap column chromatography and Superdex 200 gel filtration	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol	-	Bacillus subtilis	CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol	-	?
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol	-	Bacillus subtilis 168	CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol	-	?

Subunits

Subunits	Comment	Organism
?	x * 47079, calculated from amino acid sequence	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
CDP-glycerol:N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenyl glycerophosphotransferase	-	Bacillus subtilis
Tag primase	-	Bacillus subtilis
tagB	-	Bacillus subtilis

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Release 2023.1 (January 2023)