BRENDA - Enzyme Database show
show all sequences of 2.7.8.43

A phosphoethanolamine transferase specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A

Liu, L.; Li, Y.; Wang, X.; Guo, W.; J. Appl. Microbiol. 121, 1444-1456 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene ESA_RS09200, encodes a phosphoethanolamine transferase that specifically adds a phosphoethanolamine to the 4'-phosphate residue of lipid A, but is not regulated by the PmrA-PmrB system, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain W3110
Cronobacter sakazakii
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of Cronobacter sakazakii mutant strains WLL001, a ESA_RS09200 deletion mutant, and of WLL002, WLL003 and WLL004, with mutationsof genes lpxE, ESA-RS16430, and ESA_RS16425, with or without additional deletion of ESA_RS09200. Analysis of mammalian cell invasion abilities of mutants using human enterocyte-like epithelial Caco-2 cells, overview
Cronobacter sakazakii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
diacylphosphatidylethanolamine + lipid A
Cronobacter sakazakii
the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
?
diacylphosphatidylethanolamine + lipid A
Cronobacter sakazakii BAA894
the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cronobacter sakazakii
A7MFE0
gene ESA_RS09200, formerly ESA_02008
-
Cronobacter sakazakii BAA894
A7MFE0
gene ESA_RS09200, formerly ESA_02008
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diacylphosphatidylethanolamine + lipid A
the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A
738476
Cronobacter sakazakii
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
-
?
diacylphosphatidylethanolamine + lipid A
the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A
738476
Cronobacter sakazakii BAA894
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ESA_RS09200, encodes a phosphoethanolamine transferase that specifically adds a phosphoethanolamine to the 4'-phosphate residue of lipid A, but is not regulated by the PmrA-PmrB system, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain W3110
Cronobacter sakazakii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of Cronobacter sakazakii mutant strains WLL001, a ESA_RS09200 deletion mutant, and of WLL002, WLL003 and WLL004, with mutationsof genes lpxE, ESA-RS16430, and ESA_RS16425, with or without additional deletion of ESA_RS09200. Analysis of mammalian cell invasion abilities of mutants using human enterocyte-like epithelial Caco-2 cells, overview
Cronobacter sakazakii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
diacylphosphatidylethanolamine + lipid A
Cronobacter sakazakii
the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
?
diacylphosphatidylethanolamine + lipid A
Cronobacter sakazakii BAA894
the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diacylphosphatidylethanolamine + lipid A
the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A
738476
Cronobacter sakazakii
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
-
?
diacylphosphatidylethanolamine + lipid A
the phosphoethanolamine transferase is specific for the 4'-phosphate residue of Cronobacter sakazakii lipid A
738476
Cronobacter sakazakii BAA894
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
lipid A modification is observed in strain BAA894 when the 1-phosphate residue of lipid A is removed, but disappears when the 4'-phosphate residue of lipid A was removed. When gene ESA_RS16430, the orthologous gene of Escherichia coli pmrA, is deleted in Cronobacter sakazakii strain BAA894, this phosphoethanolamine modification of lipid A is still observed, suggesting that this modification is not regulated by the PmrA-PmrB system. Compared to the wild-type strain BAA894, the ESA_RS09200 deletion mutant shows decreased resistance to cationic antimicrobial peptides, increased recognition by TLR4/MD2, and decreased ability to invade and persist in mammalian cells. Phosphoethanolamine modification of lipid A reduces recognition and killing by the host innate immune system. Analysis of mammalian cell invasion abilities of mutants using human enterocyte-like epithelial Caco-2 cells, overview
Cronobacter sakazakii
physiological function
Cronobacter sakazakii modifies its lipid A structure through the enzyme to avoid recognition by the host immune cells. Gene ESA_RS09200, encodes a phosphoethanolamine transferase that specifically adds a phosphoethanolamine to the 4'-phosphate residue of lipid A, but is not regulated by the PmrA-PmrB system. The enzyme is active in cells grown at pH 5.0, not pH 7.0. Gene ESA_RS09200, but not ESA_RS16425, is required for phosphoethanolamine addition to the lipid A in strain BAA894
Cronobacter sakazakii
General Information (protein specific)
General Information
Commentary
Organism
malfunction
lipid A modification is observed in strain BAA894 when the 1-phosphate residue of lipid A is removed, but disappears when the 4'-phosphate residue of lipid A was removed. When gene ESA_RS16430, the orthologous gene of Escherichia coli pmrA, is deleted in Cronobacter sakazakii strain BAA894, this phosphoethanolamine modification of lipid A is still observed, suggesting that this modification is not regulated by the PmrA-PmrB system. Compared to the wild-type strain BAA894, the ESA_RS09200 deletion mutant shows decreased resistance to cationic antimicrobial peptides, increased recognition by TLR4/MD2, and decreased ability to invade and persist in mammalian cells. Phosphoethanolamine modification of lipid A reduces recognition and killing by the host innate immune system. Analysis of mammalian cell invasion abilities of mutants using human enterocyte-like epithelial Caco-2 cells, overview
Cronobacter sakazakii
physiological function
Cronobacter sakazakii modifies its lipid A structure through the enzyme to avoid recognition by the host immune cells. Gene ESA_RS09200, encodes a phosphoethanolamine transferase that specifically adds a phosphoethanolamine to the 4'-phosphate residue of lipid A, but is not regulated by the PmrA-PmrB system. The enzyme is active in cells grown at pH 5.0, not pH 7.0. Gene ESA_RS09200, but not ESA_RS16425, is required for phosphoethanolamine addition to the lipid A in strain BAA894
Cronobacter sakazakii
Other publictions for EC 2.7.8.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738476
Liu
A phosphoethanolamine transfer ...
Cronobacter sakazakii, Cronobacter sakazakii BAA894
J. Appl. Microbiol.
121
1444-1456
2016
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733635
Handing
The lipooligosaccharide-modify ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090
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17
910-921
2015
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735018
Trombley
Phosphoethanolamine transferas ...
Haemophilus ducreyi, Haemophilus ducreyi ATCC 700724
PLoS ONE
10
e0124373
2015
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739106
Nowicki
Extracellular zinc induces pho ...
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166-178
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1
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1
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740532
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46
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1
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738368
Packiam
Phosphoethanolamine decoration ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090
Infect. Immun.
82
2170-2179
2014
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1
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2
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10
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2
2
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739495
Piek
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9
e106513
2014
1
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739786
Fage
Crystallographic study of the ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Acta Crystallogr. Sect. D
70
2730-2739
2014
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6
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6
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12
2
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3
3
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733965
Lewis
Phosphoethanolamine residues o ...
Neisseria gonorrhoeae
Infect. Immun.
81
33-42
2013
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2
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733966
Cullen
EptC of Campylobacter jejuni m ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Infect. Immun.
81
430-4440
2013
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2
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734476
Wanty
The structure of the neisseria ...
Neisseria meningitidis, Neisseria meningitidis NMB
J. Mol. Biol.
425
3389-3402
2013
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740134
Bontemps-Gallo
Biosynthesis of osmoregulated ...
Escherichia coli
BioMed Res. Int.
2013
371429
2013
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1
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740516
Knirel
O-antigen structure of Shigell ...
Shigella flexneri
Glycobiology
23
475-485
2013
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733017
Anandan
Cloning, expression, purificat ...
Neisseria meningitidis, Neisseria meningitidis NMB
Acta Crystallogr. Sect. F
68
1494-1497
2012
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734190
Cullen
Trent, M.S.: Characterization ...
Campylobacter jejuni, Campylobacter jejuni 81-176
J. Biol. Chem.
287
326-3336
2012
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734198
Farizano
The PmrAB system-inducing cond ...
Salmonella enterica 14028s, Salmonella enterica
J. Biol. Chem.
287
38778-38789
2012
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733107
Beceiro
Phosphoethanolamine modificati ...
Acinetobacter baumannii
Antimicrob. Agents Chemother.
55
3370-3379
2011
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3
3
1
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723212
Herrera
Activation of PmrA inhibits Lp ...
Escherichia coli, Salmonella enterica, Salmonella enterica LT2
Mol. Microbiol.
76
1444-1460
2010
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5
2
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735157
Cullen
A link between the assembly of ...
Campylobacter jejuni
Proc. Natl. Acad. Sci. USA
107
5160-5165
2010
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1
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4
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5
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2
2
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733963
Lewis
Phosphoethanolamine substituti ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae FA19
Infect. Immun.
77
1112-1120
2009
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-
1
-
1
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-
-
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4
-
3
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6
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1
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1
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4
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6
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1
1
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733962
Takahashi
Modification of lipooligosacch ...
Neisseria meningitidis
Infect. Immun.
76
5777-5789
2008
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1
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1
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4
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1
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1
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1
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1
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2
2
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734067
Tamayo
Identification of cptA, a PmrA ...
Salmonella enterica, Salmonella enterica LT2
J. Bacteriol.
187
3391-3399
2005
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1
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4
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5
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4
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2
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4
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4
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2
4
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734151
Tran
Resistance to the antimicrobia ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium C5
J. Biol. Chem.
280
28186-28194
2005
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1
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3
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1
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-
-
-
-
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5
5
-
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734064
Lee
The PmrA-regulated pmrC gene m ...
Salmonella enterica
J. Bacteriol.
186
4124-4133
2004
1
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1
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1
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3
3
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734148
Tran
Periplasmic cleavage and modif ...
Helicobacter pylori
J. Biol. Chem.
279
55780-55791
2004
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1
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3
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4
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3
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1
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3
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3
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2
2
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734062
Cox
Phosphorylation of the lipid A ...
Neisseria meningitidis
J. Bacteriol.
185
3270-3277
2003
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1
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1
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1
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3
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1
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1
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1
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1
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1
-
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-
-
-
-
-
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2
2
-
-
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734138
Zhou
Lipid A modifications in polym ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028
J. Biol. Chem.
276
43111-43121
2001
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1
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2
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2
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2
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1
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2
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2
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-
-
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1
1
-
-
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734706
Gunn
PmrA-PmrB-regulated genes nece ...
Salmonella enterica 14028s, Salmonella enterica
Mol. Microbiol.
27
1171-1182
1998
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1
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3
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1
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2
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