BRENDA - Enzyme Database show
show all sequences of 2.7.8.43

Phosphoethanolamine residues on the lipid A moiety of Neisseria gonorrhoeae lipooligosaccharide modulate binding of complement inhibitors and resistance to complement killing

Lewis, L.A.; Shafer, W.M.; Dutta Ray, T.; Ram, S.; Rice, P.A.; Infect. Immun. 81, 33-42 (2013)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neisseria gonorrhoeae
-
gene lptA
-
General Information
General Information
Commentary
Organism
malfunction
loss of phosphoethanolamine from lipid A diminishes binding of the complement regulatory protein C4b binding protein (C4BP) to the FA19 porin B (PorB), providing a molecular basis to explain the susceptibility of an lptA null strain of FA19 to killing by normal human serum. Loss of phosphoethanolamine from lipid A also affects binding of the alternative pathway regulator factor H to PorB of some strains, e.g. strains 252 and 1291, but not of strains FA1090 and 273. Complementation of lptA null strains with lptA restores C4BP binding, decreased C4b deposition, and increased resistance to killing by normal human serum
Neisseria gonorrhoeae
physiological function
phosphoethanolamine-lipid A contributes to serum resistance by modulating factor H binding
Neisseria gonorrhoeae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
loss of phosphoethanolamine from lipid A diminishes binding of the complement regulatory protein C4b binding protein (C4BP) to the FA19 porin B (PorB), providing a molecular basis to explain the susceptibility of an lptA null strain of FA19 to killing by normal human serum. Loss of phosphoethanolamine from lipid A also affects binding of the alternative pathway regulator factor H to PorB of some strains, e.g. strains 252 and 1291, but not of strains FA1090 and 273. Complementation of lptA null strains with lptA restores C4BP binding, decreased C4b deposition, and increased resistance to killing by normal human serum
Neisseria gonorrhoeae
physiological function
phosphoethanolamine-lipid A contributes to serum resistance by modulating factor H binding
Neisseria gonorrhoeae
Other publictions for EC 2.7.8.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1444-1456
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910-921
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735018
Trombley
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PLoS ONE
10
e0124373
2015
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Nowicki
Extracellular zinc induces pho ...
Pseudomonas aeruginosa
Mol. Microbiol.
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166-178
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740532
Telke
Functional genomics to discove ...
Shewanella algae, Shewanella algae MARS 14
Int. J. Antimicrob. Agents
46
648-652
2015
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738368
Packiam
Phosphoethanolamine decoration ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090
Infect. Immun.
82
2170-2179
2014
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1
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10
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2
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739495
Piek
The role of oxidoreductases in ...
Neisseria meningitidis, Neisseria meningitidis NMB
PLoS ONE
9
e106513
2014
1
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1
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1
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739786
Fage
Crystallographic study of the ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Acta Crystallogr. Sect. D
70
2730-2739
2014
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1
1
6
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733965
Lewis
Phosphoethanolamine residues o ...
Neisseria gonorrhoeae
Infect. Immun.
81
33-42
2013
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2
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733966
Cullen
EptC of Campylobacter jejuni m ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Infect. Immun.
81
430-4440
2013
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1
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1
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734476
Wanty
The structure of the neisseria ...
Neisseria meningitidis, Neisseria meningitidis NMB
J. Mol. Biol.
425
3389-3402
2013
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1
1
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12
1
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4
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740134
Bontemps-Gallo
Biosynthesis of osmoregulated ...
Escherichia coli
BioMed Res. Int.
2013
371429
2013
1
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1
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1
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740516
Knirel
O-antigen structure of Shigell ...
Shigella flexneri
Glycobiology
23
475-485
2013
-
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1
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1
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733017
Anandan
Cloning, expression, purificat ...
Neisseria meningitidis, Neisseria meningitidis NMB
Acta Crystallogr. Sect. F
68
1494-1497
2012
-
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1
1
1
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1
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7
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734190
Cullen
Trent, M.S.: Characterization ...
Campylobacter jejuni, Campylobacter jejuni 81-176
J. Biol. Chem.
287
326-3336
2012
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2
2
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734198
Farizano
The PmrAB system-inducing cond ...
Salmonella enterica 14028s, Salmonella enterica
J. Biol. Chem.
287
38778-38789
2012
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3
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733107
Beceiro
Phosphoethanolamine modificati ...
Acinetobacter baumannii
Antimicrob. Agents Chemother.
55
3370-3379
2011
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3
3
1
-
-
723212
Herrera
Activation of PmrA inhibits Lp ...
Escherichia coli, Salmonella enterica, Salmonella enterica LT2
Mol. Microbiol.
76
1444-1460
2010
1
-
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2
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2
1
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5
2
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735157
Cullen
A link between the assembly of ...
Campylobacter jejuni
Proc. Natl. Acad. Sci. USA
107
5160-5165
2010
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733963
Lewis
Phosphoethanolamine substituti ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae FA19
Infect. Immun.
77
1112-1120
2009
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733962
Takahashi
Modification of lipooligosacch ...
Neisseria meningitidis
Infect. Immun.
76
5777-5789
2008
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1
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734067
Tamayo
Identification of cptA, a PmrA ...
Salmonella enterica, Salmonella enterica LT2
J. Bacteriol.
187
3391-3399
2005
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1
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734151
Tran
Resistance to the antimicrobia ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium C5
J. Biol. Chem.
280
28186-28194
2005
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5
5
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734064
Lee
The PmrA-regulated pmrC gene m ...
Salmonella enterica
J. Bacteriol.
186
4124-4133
2004
1
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3
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734148
Tran
Periplasmic cleavage and modif ...
Helicobacter pylori
J. Biol. Chem.
279
55780-55791
2004
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