BRENDA - Enzyme Database show
show all sequences of 2.7.8.43

Modification of lipooligosaccharide with phosphoethanolamine by LptA in Neisseria meningitidis enhances meningococcal adhesion to human endothelial and epithelial cells

Takahashi, H.; Carlson, R.W.; Muszynski, A.; Choudhury, B.; Kim, K.S.; Stephens, D.S.; Watanabe, H.; Infect. Immun. 76, 5777-5789 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene lptA, expression from a a multicopy plasmid in Neisseria meningitidis, construction of an lptA-FLAG epitope-tagged fusion Neisseria meningitidis strain HT1295 from strain H44/76
Neisseria meningitidis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
diacylphosphatidylethanolamine + lipid A
Neisseria meningitidis
addition of phosphoethanolamine to the phosphate group at the 4'-position of lipid A
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neisseria meningitidis
X5ELY0
diverse strains, gene lptA
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diacylphosphatidylethanolamine + lipid A
addition of phosphoethanolamine to the phosphate group at the 4'-position of lipid A
733962
Neisseria meningitidis
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
gene lptA, expression from a a multicopy plasmid in Neisseria meningitidis, construction of an lptA-FLAG epitope-tagged fusion Neisseria meningitidis strain HT1295 from strain H44/76
Neisseria meningitidis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
diacylphosphatidylethanolamine + lipid A
Neisseria meningitidis
addition of phosphoethanolamine to the phosphate group at the 4'-position of lipid A
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diacylphosphatidylethanolamine + lipid A
addition of phosphoethanolamine to the phosphate group at the 4'-position of lipid A
733962
Neisseria meningitidis
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
disruption of gene lptA leads to a approximately 10fold decrease in Neisseria meningitidis adhesion to four kinds of human endothelial and epithelial cell lines. Complementation with the lptA gene in the DELTAlptA mutant restores wild-type adherence
Neisseria meningitidis
physiological function
modification of lipooligosaccharide with phosphoethanolamine by enzyme LptA enhances meningococcal adhesion to human endothelial and epithelial cells in unencapsulated Neisseria meningitidis. LptA does not directly act as an adhesin molecule. Enzyme LptA-mediated adhesion may be masked when meningococci are encapsulated
Neisseria meningitidis
General Information (protein specific)
General Information
Commentary
Organism
malfunction
disruption of gene lptA leads to a approximately 10fold decrease in Neisseria meningitidis adhesion to four kinds of human endothelial and epithelial cell lines. Complementation with the lptA gene in the DELTAlptA mutant restores wild-type adherence
Neisseria meningitidis
physiological function
modification of lipooligosaccharide with phosphoethanolamine by enzyme LptA enhances meningococcal adhesion to human endothelial and epithelial cells in unencapsulated Neisseria meningitidis. LptA does not directly act as an adhesin molecule. Enzyme LptA-mediated adhesion may be masked when meningococci are encapsulated
Neisseria meningitidis
Other publictions for EC 2.7.8.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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735018
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2015
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739106
Nowicki
Extracellular zinc induces pho ...
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Mol. Microbiol.
97
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2015
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740532
Telke
Functional genomics to discove ...
Shewanella algae, Shewanella algae MARS 14
Int. J. Antimicrob. Agents
46
648-652
2015
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738368
Packiam
Phosphoethanolamine decoration ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090
Infect. Immun.
82
2170-2179
2014
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1
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10
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2
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739495
Piek
The role of oxidoreductases in ...
Neisseria meningitidis, Neisseria meningitidis NMB
PLoS ONE
9
e106513
2014
1
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1
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1
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8
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739786
Fage
Crystallographic study of the ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Acta Crystallogr. Sect. D
70
2730-2739
2014
-
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1
1
6
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1
1
1
7
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3
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1
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12
2
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6
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12
2
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3
3
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733965
Lewis
Phosphoethanolamine residues o ...
Neisseria gonorrhoeae
Infect. Immun.
81
33-42
2013
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2
2
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733966
Cullen
EptC of Campylobacter jejuni m ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Infect. Immun.
81
430-4440
2013
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1
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734476
Wanty
The structure of the neisseria ...
Neisseria meningitidis, Neisseria meningitidis NMB
J. Mol. Biol.
425
3389-3402
2013
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1
1
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4
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740134
Bontemps-Gallo
Biosynthesis of osmoregulated ...
Escherichia coli
BioMed Res. Int.
2013
371429
2013
1
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740516
Knirel
O-antigen structure of Shigell ...
Shigella flexneri
Glycobiology
23
475-485
2013
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733017
Anandan
Cloning, expression, purificat ...
Neisseria meningitidis, Neisseria meningitidis NMB
Acta Crystallogr. Sect. F
68
1494-1497
2012
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734190
Cullen
Trent, M.S.: Characterization ...
Campylobacter jejuni, Campylobacter jejuni 81-176
J. Biol. Chem.
287
326-3336
2012
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734198
Farizano
The PmrAB system-inducing cond ...
Salmonella enterica 14028s, Salmonella enterica
J. Biol. Chem.
287
38778-38789
2012
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733107
Beceiro
Phosphoethanolamine modificati ...
Acinetobacter baumannii
Antimicrob. Agents Chemother.
55
3370-3379
2011
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723212
Herrera
Activation of PmrA inhibits Lp ...
Escherichia coli, Salmonella enterica, Salmonella enterica LT2
Mol. Microbiol.
76
1444-1460
2010
1
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735157
Cullen
A link between the assembly of ...
Campylobacter jejuni
Proc. Natl. Acad. Sci. USA
107
5160-5165
2010
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733963
Lewis
Phosphoethanolamine substituti ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae FA19
Infect. Immun.
77
1112-1120
2009
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1
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1
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733962
Takahashi
Modification of lipooligosacch ...
Neisseria meningitidis
Infect. Immun.
76
5777-5789
2008
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1
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2
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734067
Tamayo
Identification of cptA, a PmrA ...
Salmonella enterica, Salmonella enterica LT2
J. Bacteriol.
187
3391-3399
2005
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1
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734151
Tran
Resistance to the antimicrobia ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium C5
J. Biol. Chem.
280
28186-28194
2005
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The PmrA-regulated pmrC gene m ...
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Phosphorylation of the lipid A ...
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Lipid A modifications in polym ...
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