BRENDA - Reference to 2.7.8.43; Id = 733017

recombinant expression of His-tagged membrane-deletion mutant enzyme in Escherichia coli strain BL21(DE3)pLysS

Neisseria meningitidis

recombinant His-tagged membrane-deletion mutant enzyme, mixing of 0.001 ml of 5.2 mg/ml protein in 50 mM HEPES, pH 8.0, 50 mM sodium chloride, with 0.001 ml of crystallization solution containing 23-26% PEG 8000, 100 mM ammonium sulfate, 100 mM HEPES, pH 7.5–8.0, 15 mM n-dodecyl-N,N-dimethylamine-N-oxide, and 200 nl of microseeding solution, 20°C, 3-5 days, method optimization, X-ray diffraction structure determination and analysis at 1.7 A resolution

Neisseria meningitidis

additional information

construction of a membrane-deletion mutant of the enzyme

Neisseria meningitidis

periplasm

-

Neisseria meningitidis

-

Neisseria meningitidis

Q7DDQ9

gene lptA

-

Neisseria meningitidis NMB

Q7DDQ9

gene lptA

-

recombinant His-tagged membrane-deletion mutant enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis

Neisseria meningitidis

More

the enzyme is composed of an N-terminal transmembrane domain and a C-terminal soluble domain. Tryptic peptide mapping and analysis by MALDI-TOF/TOF mass spectrometry

Neisseria meningitidis

recombinant expression of His-tagged membrane-deletion mutant enzyme in Escherichia coli strain BL21(DE3)pLysS

Neisseria meningitidis

recombinant His-tagged membrane-deletion mutant enzyme, mixing of 0.001 ml of 5.2 mg/ml protein in 50 mM HEPES, pH 8.0, 50 mM sodium chloride, with 0.001 ml of crystallization solution containing 23-26% PEG 8000, 100 mM ammonium sulfate, 100 mM HEPES, pH 7.5–8.0, 15 mM n-dodecyl-N,N-dimethylamine-N-oxide, and 200 nl of microseeding solution, 20°C, 3-5 days, method optimization, X-ray diffraction structure determination and analysis at 1.7 A resolution

Neisseria meningitidis

additional information

construction of a membrane-deletion mutant of the enzyme

Neisseria meningitidis

periplasm

-

Neisseria meningitidis

-

recombinant His-tagged membrane-deletion mutant enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis

Neisseria meningitidis

More

the enzyme is composed of an N-terminal transmembrane domain and a C-terminal soluble domain. Tryptic peptide mapping and analysis by MALDI-TOF/TOF mass spectrometry

Neisseria meningitidis

physiological function

the enzyme phosphoethanolamine transferase A is involved in the addition of phosphoethanolamine moieties to lipid A

Neisseria meningitidis

physiological function

the enzyme phosphoethanolamine transferase A is involved in the addition of phosphoethanolamine moieties to lipid A

Neisseria meningitidis

738476

Liu

A phosphoethanolamine transfer ...

Cronobacter sakazakii, Cronobacter sakazakii BAA894

J. Appl. Microbiol.

121

1444-1456

2016

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1

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4

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2

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1

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2

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733635

Handing

The lipooligosaccharide-modify ...

Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090

Cell. Microbiol.

17

910-921

2015

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1

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1

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2

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4

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2

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1

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1

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2

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2

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2

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735018

Trombley

Phosphoethanolamine transferas ...

Haemophilus ducreyi, Haemophilus ducreyi ATCC 700724

PLoS ONE

10

e0124373

2015

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1

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1

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1

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7

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5

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739106

Nowicki

Extracellular zinc induces pho ...

Pseudomonas aeruginosa

Mol. Microbiol.

97

166-178

2015

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1

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1

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1

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1

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4

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2

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1

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740532

Telke

Functional genomics to discove ...

Shewanella algae, Shewanella algae MARS 14

Int. J. Antimicrob. Agents

46

648-652

2015

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1

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2

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2

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4

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1

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2

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4

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1

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738368

Packiam

Phosphoethanolamine decoration ...

Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090

Infect. Immun.

82

2170-2179

2014

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1

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2

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10

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2

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1

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2

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2

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2

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739495

Piek

The role of oxidoreductases in ...

Neisseria meningitidis, Neisseria meningitidis NMB

PLoS ONE

9

e106513

2014

1

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1

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1

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1

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8

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1

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739786

Fage

Crystallographic study of the ...

Campylobacter jejuni, Campylobacter jejuni 81-176

Acta Crystallogr. Sect. D

70

2730-2739

2014

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1

6

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1

7

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3

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1

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12

2

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1

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6

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12

2

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733965

Lewis

Phosphoethanolamine residues o ...

Neisseria gonorrhoeae

Infect. Immun.

81

33-42

2013

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1

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2

-

733966

Cullen

EptC of Campylobacter jejuni m ...

Campylobacter jejuni, Campylobacter jejuni 81-176

Infect. Immun.

81

430-4440

2013

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1

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1

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8

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3

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8

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1

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1

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8

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8

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2

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734476

Wanty

The structure of the neisseria ...

Neisseria meningitidis, Neisseria meningitidis NMB

J. Mol. Biol.

425

3389-3402

2013

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1

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1

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3

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6

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8

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1

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12

1

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5

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6

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1

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12

1

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4

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740134

Bontemps-Gallo

Biosynthesis of osmoregulated ...

Escherichia coli

BioMed Res. Int.

2013

371429

2013

1

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740516

Knirel

O-antigen structure of Shigell ...

Shigella flexneri

Glycobiology

23

475-485

2013

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3

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5

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1

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3

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5

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1

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733017

Anandan

Cloning, expression, purificat ...

Neisseria meningitidis, Neisseria meningitidis NMB

Acta Crystallogr. Sect. F

68

1494-1497

2012

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1

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1

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7

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1

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1

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1

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1

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1

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1

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1

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1

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734190

Cullen

Trent, M.S.: Characterization ...

Campylobacter jejuni, Campylobacter jejuni 81-176

J. Biol. Chem.

287

326-3336

2012

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6

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7

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8

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6

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8

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2

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734198

Farizano

The PmrAB system-inducing cond ...

Salmonella enterica 14028s, Salmonella enterica

J. Biol. Chem.

287

38778-38789

2012

-

3

-

3

-

733107

Beceiro

Phosphoethanolamine modificati ...

Acinetobacter baumannii

Antimicrob. Agents Chemother.

55

3370-3379

2011

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1

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1

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1

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1

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1

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1

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1

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1

3

1

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723212

Herrera

Activation of PmrA inhibits Lp ...

Escherichia coli, Salmonella enterica, Salmonella enterica LT2

Mol. Microbiol.

76

1444-1460

2010

1

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2

1

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3

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2

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735157

Cullen

A link between the assembly of ...

Campylobacter jejuni

Proc. Natl. Acad. Sci. USA

107

5160-5165

2010

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1

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1

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1

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4

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2

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5

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1

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5

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2

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733963

Lewis

Phosphoethanolamine substituti ...

Neisseria gonorrhoeae, Neisseria gonorrhoeae FA19

Infect. Immun.

77

1112-1120

2009

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1

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1

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4

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3

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6

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1

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1

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4

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6

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1

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733962

Takahashi

Modification of lipooligosacch ...

Neisseria meningitidis

Infect. Immun.

76

5777-5789

2008

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1

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1

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4

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1

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1

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1

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1

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2

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734067

Tamayo

Identification of cptA, a PmrA ...

Salmonella enterica, Salmonella enterica LT2

J. Bacteriol.

187

3391-3399

2005

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1

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4

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5

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2

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4

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4

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734151

Tran

Resistance to the antimicrobia ...

Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium C5

J. Biol. Chem.

280

28186-28194

2005

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1

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3

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1

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5

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734064

Lee

The PmrA-regulated pmrC gene m ...

Salmonella enterica

J. Bacteriol.

186

4124-4133

2004

1

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1

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1

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3

-

734148

Tran

Periplasmic cleavage and modif ...

Helicobacter pylori

J. Biol. Chem.

279

55780-55791

2004

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1

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3

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1

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3

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3

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734062

Cox

Phosphorylation of the lipid A ...

Neisseria meningitidis

J. Bacteriol.

185

3270-3277

2003

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1

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1

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1

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3

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734138

Zhou

Lipid A modifications in polym ...

Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028

J. Biol. Chem.

276

43111-43121

2001

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2

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2

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1

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2

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1

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734706

Gunn

PmrA-PmrB-regulated genes nece ...

Salmonella enterica 14028s, Salmonella enterica

Mol. Microbiol.

27

1171-1182

1998

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1

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3

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Cloning, expression, purification and crystallization of an endotoxin-biosynthesis enzyme from Neisseria meningitidis

Data extracted from this reference: