Cloned (Comment) | Organism |
---|---|
gene CrCLS1, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR expression analysis, the gene cannot perform functional complementation of pgsA, a PGPS mutant of Synechocystis sp. PCC6803, but of DELTAcrd1, a CLS mutant of Saccharomyces cerevisiae | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | - |
Chlamydomonas reinhardtii | 31966 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a CDP-diacylglycerol + a phosphatidylglycerol | Chlamydomonas reinhardtii | - |
a cardiolipin + CMP | - |
? | |
a CDP-diacylglycerol + a phosphatidylglycerol | Chlamydomonas reinhardtii CC-503 | - |
a cardiolipin + CMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | A8JAW5 | cw92mt+ | - |
Chlamydomonas reinhardtii CC-503 | A8JAW5 | cw92mt+ | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a CDP-diacylglycerol + a phosphatidylglycerol | - |
Chlamydomonas reinhardtii | a cardiolipin + CMP | - |
? | |
a CDP-diacylglycerol + a phosphatidylglycerol | - |
Chlamydomonas reinhardtii CC-503 | a cardiolipin + CMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cardiolipin synthase | - |
Chlamydomonas reinhardtii |
CHLREDRAFT_177689 | - |
Chlamydomonas reinhardtii |
CLS | - |
Chlamydomonas reinhardtii |
CrCLS1 | - |
Chlamydomonas reinhardtii |
Cre13.g604700 | locus name | Chlamydomonas reinhardtii |
phosphatidylglycerophosphate synthase-like protein | UniProt | Chlamydomonas reinhardtii |
General Information | Comment | Organism |
---|---|---|
evolution | phosphatidylglycerophosphate synthase (PGPS) and cardiolipin synthase (CLS) are both involved in the biosynthesis of phosphatidylglycerol and cardiolipin and belong to the CDP-alcohol phosphotransferases, they share overall amino acid sequence homology. PGPS and CLS are functionally distinct in vivo. Comparison of CDP-alcohol phosphotransferase motifs between PGPS and CLS among different species reveal a possible additional motif that might define the substrate specificity of the closely related enzymes | Chlamydomonas reinhardtii |
metabolism | cardiolipin synthase is involved in the biosynthesis of cardiolipin | Chlamydomonas reinhardtii |
physiological function | gene CrCLS1 does not complement the growth phenotype of a phosphatidylglycerophosphate synthase (PGPS) mutant of Synechocystis sp. PCC6803, but it rescues the temperature-sensitive growth phenotype, growth profile with different carbon sources, phospholipid composition, and enzyme activity of DELTAcrd1, a cardiolipin synthase (CLS) mutant of Saccharomyces cerevisiae | Chlamydomonas reinhardtii |