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Literature summary for 2.7.8.4 extracted from

  • Allen, A.K.; Rosenberg, H.
    The mechanism of action and some properties of serine ethanolamine phosphate synthetase (1968), Biochim. Biophys. Acta, 151, 504-519.
    View publication on PubMed
Search for more literature for 2.7.8.4

General Stability

General Stability Organism
stable to freezing, aggregates on thawing Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Gallus gallus
CDP strong inhibition Gallus gallus
CMP competitive inhibition to CDPethanolamine, non-competitive inhibition to L-serine Gallus gallus
iodoacetamide 1 mM, 6% remaining activity Gallus gallus
iodoacetate 1 mM, 42% remaining activity Gallus gallus
L-alanine 27-34% inhibition of the reaction Gallus gallus
L-homoserine 27-34% inhibition of the reaction Gallus gallus
L-threonine 27-34% inhibition of the reaction Gallus gallus
Methylmercuric bromide 0.1 mM, 6% remaining activity Gallus gallus
Methylmercuric iodide 0.1 mM, 3% remaining activity Gallus gallus
additional information iodoacetate is less effective as inhibitor, DFP is no inhibitor Gallus gallus
N-Acetylimidazole 10 mM, 8% remaining activity Gallus gallus
N-ethylmaleimide 0.2 mM, 25% remaining activity Gallus gallus
p-hydroxymercuribenzoate 0.1 mM, 2% remaining activity Gallus gallus
phenylmercuric acetate 0.1 mM, 2% remaining activity Gallus gallus
Sulfanilic acid diazotized, 0.2 mM, 36% remaining activity Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 CMP-aminoethylphosphonate pH 7.0, 37°C Gallus gallus
0.085
-
 CDP-ethanolamine pH 7.0, 37°C Gallus gallus
1
-
 L-serine pH 7.0, 37°C Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Gallus gallus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Gallus gallus
Mg2+ bivalent cations are essential for activity, Mg2+ is most effective Gallus gallus
Mn2+
-
 Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CDP-ethanolamine + L-serine Gallus gallus
-
 CMP + L-serine-ethanolamine phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 chicken
-

Purification (Commentary)

Purification (Comment) Organism
partial Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
gut
-
 Gallus gallus
-
intestinal mucosa
-
 Gallus gallus
-
kidney
-
 Gallus gallus
-
additional information no activity in liver, spleen, brain, oviduct, blood, and heart Gallus gallus
-
pancreas
-
 Gallus gallus
-

Storage Stability

Storage Stability Organism
0°C, stable to freezing Gallus gallus
4°C, enzyme preparartion remains active Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-2-amino-2-methylpropanol + L-serine
-
 Gallus gallus ?
-
 ?
CDP-ethanolamine + DL-alpha-methylserine
-
 Gallus gallus ?
-
 ?
CDP-ethanolamine + L-serine
-
 Gallus gallus CMP + L-serine-ethanolamine phosphate
-
 ?
CDP-ethanolamine + L-serine absolutely specific for serine, both stereoisomers are active Gallus gallus CMP + L-serine-ethanolamine phosphate
-
 ?
CMP-aminoethylphosphonate + L-serine
-
 Gallus gallus ?
-
 ?
additional information CDP-choline and CDP-serine cannot replace CDP-ethanolamine, N-acetyl-L-serine, L-homoserine, 4-hydroxy-L-proline, 5-hydroxy-DL-lysine, 3-hydroxy-DL-glutamic acid, L-threonine, ethanolamine or 3-hydroxypropionic acid cannot replace serine Gallus gallus ?
-
 ?

Synonyms

Synonyms Comment Organism
SEP synthetase
-
 Gallus gallus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable for 60 min Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 55% of maximal activity at pH 6.5, 30% of maximal activity at pH 9.0 Gallus gallus