BRENDA - Enzyme Database show
show all sequences of 2.7.8.33

Characterization of the highly conserved VFMGD motif in a bacterial polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase

Furlong, S.E.; Valvano, M.A.; Protein Sci. 21, 1366-1375 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli MV501 cells
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
D217A
the mutation results in reduced enzymatic activity
Escherichia coli
D217E
the mutation results in a slightly more active enzyme
Escherichia coli
D217K
inactive
Escherichia coli
D217N
the mutation results in a more active enzyme and leads to more than 2fold increase in Vmax without significant change in the Km for the UDP-N-acetyl-D-glucosamine
Escherichia coli
D217S
the mutation results in a more active enzyme
Escherichia coli
F214A
the mutation results in severely reduced enzymatic activity
Escherichia coli
G216A
inactive
Escherichia coli
M215A
the mutation results in a slightly more active enzyme
Escherichia coli
V213A
the mutation results in severely reduced enzymatic activity
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
tunicamycin
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00007
-
UDP-N-acetyl-D-glucosamine
mutant enzyme, in 50 mM of Tris-HCl (pH 8.0), 40 mM of MgCl2, at 37C
Escherichia coli
0.00008
-
UDP-N-acetyl-D-glucosamine
wild type enzyme, in 50 mM of Tris-HCl (pH 8.0), 40 mM of MgCl2, at 37C
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
Co2+ affinity chromatography
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate
-
723725
Escherichia coli
UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli MV501 cells
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D217A
the mutation results in reduced enzymatic activity
Escherichia coli
D217E
the mutation results in a slightly more active enzyme
Escherichia coli
D217K
inactive
Escherichia coli
D217N
the mutation results in a more active enzyme and leads to more than 2fold increase in Vmax without significant change in the Km for the UDP-N-acetyl-D-glucosamine
Escherichia coli
D217S
the mutation results in a more active enzyme
Escherichia coli
F214A
the mutation results in severely reduced enzymatic activity
Escherichia coli
G216A
inactive
Escherichia coli
M215A
the mutation results in a slightly more active enzyme
Escherichia coli
V213A
the mutation results in severely reduced enzymatic activity
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
tunicamycin
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00007
-
UDP-N-acetyl-D-glucosamine
mutant enzyme, in 50 mM of Tris-HCl (pH 8.0), 40 mM of MgCl2, at 37C
Escherichia coli
0.00008
-
UDP-N-acetyl-D-glucosamine
wild type enzyme, in 50 mM of Tris-HCl (pH 8.0), 40 mM of MgCl2, at 37C
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
Co2+ affinity chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate
-
723725
Escherichia coli
UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
-
-
?
Other publictions for EC 2.7.8.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737788
Al-Dabbagh
Catalytic mechanism of MraY an ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
Biochimie
127
249-257
2016
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1
-
1
-
1
-
1
1
-
2
-
2
-
-
1
1
-
-
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6
-
1
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1
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1
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1
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1
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1
1
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2
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1
-
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-
6
-
1
-
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-
1
-
-
-
-
3
3
-
-
-
738596
Ishizaki
Inhibition of the first step i ...
Bacillus subtilis 168, Bacillus subtilis
J. Biol. Chem.
288
30309-30319
2013
-
-
-
-
1
-
3
-
-
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2
-
4
-
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2
-
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2
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1
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3
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2
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-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
723725
Furlong
Characterization of the highly ...
Escherichia coli
Protein Sci.
21
1366-1375
2012
-
-
1
-
9
-
1
2
1
1
-
-
-
2
-
-
1
-
-
-
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1
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1
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9
-
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1
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2
1
1
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1
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1
-
-
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-
-
-
-
-
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-
-
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-
722351
Hug
Analogies and homologies in li ...
Escherichia coli
Glycobiology
21
138-151
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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1
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1
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710401
Hug
Helicobacter pylori lipopolysa ...
Helicobacter pylori
PLoS Pathog.
6
e1000819
2010
-
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1
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1
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-
707104
Al-Dabbagh
Preparative enzymatic synthesi ...
Thermotoga maritima
Anal. Biochem.
391
163-165
2009
-
-
-
-
-
-
-
-
1
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1
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1
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1
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3
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1
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1
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1
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1
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1
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3
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1
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1
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-
-
708920
Al-Dabbagh
Purification and characterizat ...
Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
J. Bacteriol.
190
7141-7146
2008
2
-
1
-
-
-
4
2
1
1
3
2
-
2
-
-
1
-
-
-
1
-
12
1
1
1
-
2
1
1
-
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-
-
-
2
-
1
-
-
-
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-
4
-
2
1
1
3
2
-
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1
-
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1
-
12
1
1
1
-
2
1
1
-
-
-
-
-
-
-
-
708918
Lehrer
Functional characterization an ...
Escherichia coli
J. Bacteriol.
189
2618-2628
2007
-
-
1
-
8
-
-
2
1
2
3
1
-
1
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
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1
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1
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-
8
-
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2
1
2
3
1
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1
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2
1
1
-
-
-
1
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-
1
-
-
-
-
-
-
709837
Amer
Conserved amino acid residues ...
Escherichia coli
Microbiology
147
3015-3025
2001
-
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1
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1
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708621
Rush
Polyisoprenyl phosphate specif ...
Escherichia coli
Glycobiology
7
315-322
1997
-
-
-
-
-
-
1
1
1
1
-
-
-
6
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-
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3
-
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1
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1
1
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3
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