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Literature summary for 2.7.8.31 extracted from

  • Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Pühler, A.; Ielpi, L.
    Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence (1998), J. Bacteriol., 180, 1607-1617.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide Xanthomonas campestris

Cloned(Commentary)

Cloned (Comment) Organism
the C-terminal portion of GumD is cloned into broad-host-range vector pRK404, producing plasmid pCD2. The C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + ditrans,octacis-undecaprenyl phosphate Xanthomonas campestris the enzyme is involved in biosynthesis of xanthan UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
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Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
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 pv. campestris
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + ditrans,octacis-undecaprenyl phosphate the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
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 ?
UDP-glucose + ditrans,octacis-undecaprenyl phosphate the C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity Xanthomonas campestris UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
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 ?

General Information

General Information Comment Organism
malfunction from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumD completely abolishes in vitro polymer formation. The gumD- mutant is the unique mutant strain, generated in a wild-type background, that shows no released labeled oligosaccharides when permeabilized cells are labeled with UDP-[14C]glucose. Cells labeled with UDP-[14C]glucuronic acid or GDP-[14C]mannose show similar results. The C-terminal portion of GumD is cloned into broad-host-range vector pRK404, producing plasmid pCD2. This plasmid complements the xanthan defect in the XcD strain, rendering mucoid colonies Xanthomonas campestris
physiological function the enzyme is involved in biosynthesis of the exopolysaccharide xanthan Xanthomonas campestris