BRENDA - Enzyme Database
show all sequences of 2.7.8.29

Phosphatidylserine synthase 2: high efficiency for synthesizing phosphatidylserine containing docosahexaenoic acid

Kimura, A.K.; Kim, H.Y.; J. Lipid Res. 54, 214-222 (2013)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in HEK-293 cells; gene PTDSS2, functional recombinant overexpression of FLAG-tagged PSS2 isozyme in HEK-293 and CHO-K1 cells
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
;
Mus musculus
5783
-
membrane
; integral membrane protein; integral membrane protein
Mus musculus
16020
-
microsome
;
Mus musculus
-
-
mitochondrial membrane
;
Mus musculus
31966
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
; x * 50000, calculated from amino acid sequence
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-1-phosphatidylcholine + L-serine
Mus musculus
isozyme PSS1
L-1-phosphatidylserine + choline
-
-
r
L-1-phosphatidylethanolamine + L-serine
Mus musculus
isozyme PSS2
L-1-phosphatidylserine + ethanolamine
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Mus musculus
-
-
-
Mus musculus
Q99LH2
-
-
Mus musculus
Q9Z1X2
-
-
Purification (Commentary)
Purification (Commentary)
Organism
anti-FLAG M2 affinity gel chromatography; purification of functional recombinant FLAG-tagged PSS2 from HEK-293 and CHO-K1 cell microsomes by ultracentrifugation, immunoaffinity chromatography
Mus musculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
;
Mus musculus
-
additional information
isozyme PSS1 is ubiquitously expressed; isozyme PSS2 is expressed in a tissue-specific manner. It is highly expressed in tissues such as brain and testis, where docosahexaenoic acid (DHA, 22:6n-3) is also highly enriched
Mus musculus
-
Purkinje neuron
-
Mus musculus
-
Sertoli cell
-
Mus musculus
-
testis
;
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1,2-diacylphosphatidylethanolamine + L-serine
the substrate is 6times better utilized than phosphatidylethanolamine plasmalogen
722906
Mus musculus
?
-
-
-
?
L-1-phosphatidylcholine + L-serine
isozyme PSS1
722906
Mus musculus
L-1-phosphatidylserine + choline
-
-
-
r
L-1-phosphatidylethanolamine + L-serine
-
722906
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
?
L-1-phosphatidylethanolamine + L-serine
isozyme PSS2
722906
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
r
additional information
the enzyme synthesizes docosahexaenoic acid-phosphatidylserine much more efficiently than phosphatidylserine containing either 18:1 or 20:4 at the sn-2 position. Although no sn-1 fatty acyl preference is noted, phosphatidylserine synthase 2 exhibits significant preference toward docosahexaenoic acid compared with 18:1 or 20:4 at the sn-2 position
722906
Mus musculus
?
-
-
-
-
additional information
isozyme PSS1 shows a 2fold higher preference for sn-1 18:0 compared with 16:0 when the sn-2 position contains 18:1
722906
Mus musculus
?
-
-
-
-
additional information
the recombinant FLAG-tagged enzyme is also active with phosphatidylethanolamine plasmalogen, [C18(plasm),22:6-PS], which is six times less effective as a substrate than the corresponding ester-linked phosphatidylethanolamine species, the isozyme is active with oleic acid (OA; 18:1n-9), DHA (DHA; 22:6n-3), or arachidonic acid esters. Compounds analysis by mass spectroscopy. A carbonyl group next to the glycerol backbone in the substrate-binding pocket substantially increases the catalytic activity of PSS2. Isozyme PSS2 shows a 10fold preference for sn-1 18:0, when 22:6 is esterified at the sn-2 position. Synthesis of 18:0,22:6-PS is most efficient compared with 18:0,18:1-PS and with 18:0,20:4-PS. Substrate specificity and acyl chain preference of isozyme PS2, overview
722906
Mus musculus
?
-
-
-
-
phosphatidylethanolamine plasmalogen + L-serine
-
722906
Mus musculus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 50000, about, recombinant FLAG-tagged PSS2, SDS-PAGE; x * 50000, calculated from amino acid sequence
Mus musculus
Synonyms
Synonyms
Commentary
Organism
phosphatidylserine synthase 1
-
Mus musculus
phosphatidylserine synthase 2
-
Mus musculus
PS synthase 1
-
Mus musculus
PS synthase 2
-
Mus musculus
PSS1
-
Mus musculus
PSS2
-
Mus musculus
PTDSS2
-
Mus musculus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Mus musculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in HEK-293 cells
Mus musculus
gene PTDSS2, functional recombinant overexpression of FLAG-tagged PSS2 isozyme in HEK-293 and CHO-K1 cells
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Mus musculus
5783
-
membrane
-
Mus musculus
16020
-
membrane
integral membrane protein
Mus musculus
16020
-
microsome
-
Mus musculus
-
-
mitochondrial membrane
-
Mus musculus
31966
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
x * 50000, calculated from amino acid sequence
Mus musculus
50000
-
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-1-phosphatidylcholine + L-serine
Mus musculus
isozyme PSS1
L-1-phosphatidylserine + choline
-
-
r
L-1-phosphatidylethanolamine + L-serine
Mus musculus
isozyme PSS2
L-1-phosphatidylserine + ethanolamine
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
anti-FLAG M2 affinity gel chromatography
Mus musculus
purification of functional recombinant FLAG-tagged PSS2 from HEK-293 and CHO-K1 cell microsomes by ultracentrifugation, immunoaffinity chromatography
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Mus musculus
-
additional information
isozyme PSS1 is ubiquitously expressed
Mus musculus
-
additional information
isozyme PSS2 is expressed in a tissue-specific manner. It is highly expressed in tissues such as brain and testis, where docosahexaenoic acid (DHA, 22:6n-3) is also highly enriched
Mus musculus
-
Purkinje neuron
-
Mus musculus
-
Sertoli cell
-
Mus musculus
-
testis
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1,2-diacylphosphatidylethanolamine + L-serine
the substrate is 6times better utilized than phosphatidylethanolamine plasmalogen
722906
Mus musculus
?
-
-
-
?
L-1-phosphatidylcholine + L-serine
isozyme PSS1
722906
Mus musculus
L-1-phosphatidylserine + choline
-
-
-
r
L-1-phosphatidylethanolamine + L-serine
-
722906
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
?
L-1-phosphatidylethanolamine + L-serine
isozyme PSS2
722906
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
r
additional information
the enzyme synthesizes docosahexaenoic acid-phosphatidylserine much more efficiently than phosphatidylserine containing either 18:1 or 20:4 at the sn-2 position. Although no sn-1 fatty acyl preference is noted, phosphatidylserine synthase 2 exhibits significant preference toward docosahexaenoic acid compared with 18:1 or 20:4 at the sn-2 position
722906
Mus musculus
?
-
-
-
-
additional information
isozyme PSS1 shows a 2fold higher preference for sn-1 18:0 compared with 16:0 when the sn-2 position contains 18:1
722906
Mus musculus
?
-
-
-
-
additional information
the recombinant FLAG-tagged enzyme is also active with phosphatidylethanolamine plasmalogen, [C18(plasm),22:6-PS], which is six times less effective as a substrate than the corresponding ester-linked phosphatidylethanolamine species, the isozyme is active with oleic acid (OA; 18:1n-9), DHA (DHA; 22:6n-3), or arachidonic acid esters. Compounds analysis by mass spectroscopy. A carbonyl group next to the glycerol backbone in the substrate-binding pocket substantially increases the catalytic activity of PSS2. Isozyme PSS2 shows a 10fold preference for sn-1 18:0, when 22:6 is esterified at the sn-2 position. Synthesis of 18:0,22:6-PS is most efficient compared with 18:0,18:1-PS and with 18:0,20:4-PS. Substrate specificity and acyl chain preference of isozyme PS2, overview
722906
Mus musculus
?
-
-
-
-
phosphatidylethanolamine plasmalogen + L-serine
-
722906
Mus musculus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 50000, calculated from amino acid sequence
Mus musculus
?
x * 50000, about, recombinant FLAG-tagged PSS2, SDS-PAGE
Mus musculus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Mus musculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Mus musculus
General Information
General Information
Commentary
Organism
metabolism
phosphatidylserine is synthesized in mammalian cells by two integral membrane proteins, PS synthase 1 and 2 (PSS1 and PSS2). These enzymes catalyze the formation of phosphatidylserine by an exchange reaction in which serine replaces the head group of the corresponding substrate phospholipids, phosphatidylcholine (PC) for isozyme PSS1 and phosphatidylethanolamine (PE) for isozyme PSS2; phosphatidylserine is synthesized in mammalian cells by two integral membrane proteins, PS synthase 1 and 2 (PSS1 and PSS2). These enzymes catalyze the formation of phosphatidylserine by an exchange reaction in which serine replaces the head group of the corresponding substrate phospholipids, phosphatidylcholine (PC) for isozyme PSS1 and phosphatidylethanolamine (PE) for isozyme PSS2
Mus musculus
physiological function
isozyme PSS2 may play a key role in phosphatidylserine accumulation in brain and testis through high activity toward DHA-containing substrates that are abundant in these tissues; the enzyme plays a key role in phosphatidylserine accumulation in brain and testis through high activity toward docosahexaenoic acid-containing substrates
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
phosphatidylserine is synthesized in mammalian cells by two integral membrane proteins, PS synthase 1 and 2 (PSS1 and PSS2). These enzymes catalyze the formation of phosphatidylserine by an exchange reaction in which serine replaces the head group of the corresponding substrate phospholipids, phosphatidylcholine (PC) for isozyme PSS1 and phosphatidylethanolamine (PE) for isozyme PSS2
Mus musculus
physiological function
the enzyme plays a key role in phosphatidylserine accumulation in brain and testis through high activity toward docosahexaenoic acid-containing substrates
Mus musculus
physiological function
isozyme PSS2 may play a key role in phosphatidylserine accumulation in brain and testis through high activity toward DHA-containing substrates that are abundant in these tissues
Mus musculus
Other publictions for EC 2.7.8.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740791
Whyte
Lenz-Majewski hyperostotic dwa ...
Homo sapiens
J. Bone Miner. Res.
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606-614
2015
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741056
Sousa
Gain-of-function mutations in ...
Homo sapiens
Nat. Genet.
46
70-76
2014
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4
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722906
Kimura
Phosphatidylserine synthase 2: ...
Mus musculus
J. Lipid Res.
54
214-222
2013
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717210
Tomohiro
Purification and characterizat ...
Homo sapiens
Biochem. J.
418
421-429
2009
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693046
Arikketh
Defining the importance of pho ...
Mus musculus
J. Biol. Chem.
283
12888-12897
2008
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1
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675553
Wen
Inhibition of phosphatidylseri ...
Rattus norvegicus
J. Neurosci. Res.
85
1568-1578
2007
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693711
Guo
Neuronal specific increase of ...
Cricetulus griseus, Homo sapiens, Mus musculus
J. Mol. Neurosci.
33
67-73
2007
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660974
Yu
Resistance to UV-induced apopt ...
Cricetulus griseus
Biochem. J.
381
609-618
2004
1
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661228
Grandmaison
Externalization of phosphatidy ...
Cricetulus griseus
Biochim. Biophys. Acta
1636
1-11
2004
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717768
Kuge
Purification and characterizat ...
Cricetulus griseus
J. Biol. Chem.
278
42692-42698
2003
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717766
Bergo
Defining the importance of pho ...
Mus musculus
J. Biol. Chem.
277
47701-47708
2002
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717762
Stone
Phosphatidylserine synthase-1 ...
Cricetulus griseus, Rattus norvegicus
J. Biol. Chem.
275
34534-34540
2000
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717208
Stone
Cloning and expression of muri ...
Mus musculus
Biochem. J.
342
57-64
1999
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717761
Kuge
Control of phosphatidylserine ...
Cricetulus griseus
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