BRENDA - Enzyme Database show
show all sequences of 2.7.8.29

Control of phosphatidylserine synthase II activity in Chinese hamster ovary cells

Kuge, O.; Saito, K.; Nishijima, M.; J. Biol. Chem. 274, 23844-23849 (1999)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
R97K
in contrast to the PSS II wild-type transformant, the R97K transformant exhibits 4fold higher phosphatidylserine biosynthetic activity than that in CHO-K1 cells. The phosphatidylserine biosynthesis in the R97K transformant is not inhibited at all but elevated by the addition of phosphatidylserine
Cricetulus griseus
Inhibitors
Inhibitors
Commentary
Organism
Structure
phosphatidylserine
The phosphatidylserine synthesis in a CHO cell mutant which lacks PSS I, EC 2.7.8.8, but has normal PSS II activity, is almost completely inhibited by the addition of phosphatidylserine to the culture medium, like that in the wild-type CHO-K1 cells. The phosphatidylserine synthesis in a PSS II-overproducing stable transformant is reduced by 35% upon addition of phosphatidylserine. Residue Arg-97 is critical for the exogenous phosphatidylserine-mediated inhibition
Cricetulus griseus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cricetulus griseus
O08888
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
CHO cell
-
Cricetulus griseus
-
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R97K
in contrast to the PSS II wild-type transformant, the R97K transformant exhibits 4fold higher phosphatidylserine biosynthetic activity than that in CHO-K1 cells. The phosphatidylserine biosynthesis in the R97K transformant is not inhibited at all but elevated by the addition of phosphatidylserine
Cricetulus griseus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
phosphatidylserine
The phosphatidylserine synthesis in a CHO cell mutant which lacks PSS I, EC 2.7.8.8, but has normal PSS II activity, is almost completely inhibited by the addition of phosphatidylserine to the culture medium, like that in the wild-type CHO-K1 cells. The phosphatidylserine synthesis in a PSS II-overproducing stable transformant is reduced by 35% upon addition of phosphatidylserine. Residue Arg-97 is critical for the exogenous phosphatidylserine-mediated inhibition
Cricetulus griseus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
CHO cell
-
Cricetulus griseus
-
General Information
General Information
Commentary
Organism
physiological function
PSS II activity is inhibited by exogenous phosphatidylserine and overproduction of PSS II leads to the loss of normal control of PSS II activity by exogenous phosphatidylserine. PSS II-overproducing cells cultivated without exogenous phosphatidylserine exhibit a normal phosphatidylserine biosynthetic rate similar to that in CHO-K1 cells. Stable transformation of R97K mutant PSS II, leads to a 4fold higher phosphatidylserine biosynthetic rate
Cricetulus griseus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
PSS II activity is inhibited by exogenous phosphatidylserine and overproduction of PSS II leads to the loss of normal control of PSS II activity by exogenous phosphatidylserine. PSS II-overproducing cells cultivated without exogenous phosphatidylserine exhibit a normal phosphatidylserine biosynthetic rate similar to that in CHO-K1 cells. Stable transformation of R97K mutant PSS II, leads to a 4fold higher phosphatidylserine biosynthetic rate
Cricetulus griseus
Other publictions for EC 2.7.8.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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J. Biol. Chem.
275
34534-34540
2000
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1999
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717761
Kuge
Control of phosphatidylserine ...
Cricetulus griseus
J. Biol. Chem.
274
23844-23849
1999
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717759
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Genetic evidence that phosphat ...
Cricetulus griseus
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273
17199-17205
1998
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717758
Kuge
Cloning of a Chinese hamster o ...
Cricetulus griseus
J. Biol. Chem.
272
19133-19139
1997
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