Cloned (Comment) | Organism |
---|---|
expression in CHO cell and Sf9 cell | Cricetulus griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | O08888 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CHO cell | - |
Cricetulus griseus | - |
Synonyms | Comment | Organism |
---|---|---|
pssB | - |
Cricetulus griseus |
General Information | Comment | Organism |
---|---|---|
physiological function | introduction of the pssB cDNA into CHO-K1 cells results in striking increases in both the serine and ethanolamine base exchange activities. The pssB cDNA is incapable of increasing the choline base exchange activity. The expression of the pssB gene in Sf9 insect cells also results in striking increases in both serine and ethanolamine base exchange activities. The pssB cDNA transforms a phosphatidylserine-auxotrophic mutant of CHO-K1 cells lacking PSS I, EC 2.7.8.8, to phosphatidylserine prototrophy. The phosphatidylserine content of the resultant transformant grown without exogenous phosphatidylserine for 2 days is 4-fold that of the mutant and similar to that of CHO-K1 cells, indicating that the pssB cDNA complements the phosphatidylserine biosynthetic defect of the PSS I-lacking mutant | Cricetulus griseus |