BRENDA - Enzyme Database
show all sequences of 2.7.8.29

Purification and characterization of human phosphatidylserine synthases 1 and 2

Tomohiro, S.; Kawaguti, A.; Kawabe, Y.; Kitada, S.; Kuge, O.; Biochem. J. 418, 421-429 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in HeLa cell
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
phosphatidylserine
product inhibition
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
L-serine
pH 7.5, 37°C
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
x * 50000, SDS-PAGE of epitope-tagged recombinant protein
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9BVG9
-
-
Purification (Commentary)
Commentary
Organism
epitope-tagged recombinant protein
Homo sapiens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1
-
pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-1-phosphatidylethanolamine + L-serine
-
717210
Homo sapiens
L-1-phosphatidylserine + ethanolamine
-
-
-
?
additional information
no substrate: phosphatidylcholine
717210
Homo sapiens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 50000, SDS-PAGE of epitope-tagged recombinant protein
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in HeLa cell
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
phosphatidylserine
product inhibition
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
L-serine
pH 7.5, 37°C
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
x * 50000, SDS-PAGE of epitope-tagged recombinant protein
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
epitope-tagged recombinant protein
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1
-
pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-1-phosphatidylethanolamine + L-serine
-
717210
Homo sapiens
L-1-phosphatidylserine + ethanolamine
-
-
-
?
additional information
no substrate: phosphatidylcholine
717210
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 50000, SDS-PAGE of epitope-tagged recombinant protein
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Homo sapiens
Other publictions for EC 2.7.8.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740791
Whyte
Lenz-Majewski hyperostotic dwa ...
Homo sapiens
J. Bone Miner. Res.
30
606-614
2015
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1
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1
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2
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1
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2
2
-
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-
741056
Sousa
Gain-of-function mutations in ...
Homo sapiens
Nat. Genet.
46
70-76
2014
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1
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4
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2
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3
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2
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3
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2
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3
4
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722906
Kimura
Phosphatidylserine synthase 2: ...
Mus musculus
J. Lipid Res.
54
214-222
2013
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1
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4
-
1
2
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2
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8
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8
2
1
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1
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2
4
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717210
Tomohiro
Purification and characterizat ...
Homo sapiens
Biochem. J.
418
421-429
2009
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1
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1
1
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1
1
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1
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1
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2
1
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1
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693046
Arikketh
Defining the importance of pho ...
Mus musculus
J. Biol. Chem.
283
12888-12897
2008
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1
1
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675553
Wen
Inhibition of phosphatidylseri ...
Rattus norvegicus
J. Neurosci. Res.
85
1568-1578
2007
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1
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1
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693711
Guo
Neuronal specific increase of ...
Cricetulus griseus, Homo sapiens, Mus musculus
J. Mol. Neurosci.
33
67-73
2007
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4
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3
3
3
3
-
-
660974
Yu
Resistance to UV-induced apopt ...
Cricetulus griseus
Biochem. J.
381
609-618
2004
1
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1
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1
1
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661228
Grandmaison
Externalization of phosphatidy ...
Cricetulus griseus
Biochim. Biophys. Acta
1636
1-11
2004
-
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1
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1
1
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717768
Kuge
Purification and characterizat ...
Cricetulus griseus
J. Biol. Chem.
278
42692-42698
2003
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1
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2
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717766
Bergo
Defining the importance of pho ...
Mus musculus
J. Biol. Chem.
277
47701-47708
2002
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1
1
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717762
Stone
Phosphatidylserine synthase-1 ...
Cricetulus griseus, Rattus norvegicus
J. Biol. Chem.
275
34534-34540
2000
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4
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2
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2
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717208
Stone
Cloning and expression of muri ...
Mus musculus
Biochem. J.
342
57-64
1999
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1
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1
1
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717761
Kuge
Control of phosphatidylserine ...
Cricetulus griseus
J. Biol. Chem.
274
23844-23849
1999
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1
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1
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1
1
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717759
Saito
Genetic evidence that phosphat ...
Cricetulus griseus
J. Biol. Chem.
273
17199-17205
1998
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717758
Kuge
Cloning of a Chinese hamster o ...
Cricetulus griseus
J. Biol. Chem.
272
19133-19139
1997
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