BRENDA - Enzyme Database
show all sequences of 2.7.8.29

Cloning and expression of murine liver phosphatidylserine synthase (PSS)-2: differential regulation of phospholipid metabolism by PSS1 and PSS2

Stone, S.J.; Vance, J.E.; Biochem. J. 342, 57-64 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in McArdle cell and in ethanolamine-requiring mutant Chinese hamster ovary cells defective in PSS1, EC 2.7.8.29
Mus musculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
Q9Z1X2
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-1-phosphatidylethanolamine + L-serine
-
717208
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in McArdle cell and in ethanolamine-requiring mutant Chinese hamster ovary cells defective in PSS1, EC 2.7.8.29
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-1-phosphatidylethanolamine + L-serine
-
717208
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
expression of PSS2 in ethanolamine-requiring mutant Chinese hamster ovary cells defective in PSS1, reverses the ethanolamine auxotrophy. However, the phosphatidylethanolamine content is not normalized unless the culture medium is supplemented with ethanolamine. In both mutant chinese hamster ovary cells and hepatoma cells transfected with PSS2 cDNA the rate of synthesis of phosphatidylserine and phosphatidylserine-derived phosphatidylethanolamine does not exceed that in parental chinese hamster ovary cells or control McArdle cells, respectively. Expression of murine PSS2 in McArdle cells does not inhibit phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway, whereas expression of similar levels of PSS1 activity inhibit this pathway by approx. 50%
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
expression of PSS2 in ethanolamine-requiring mutant Chinese hamster ovary cells defective in PSS1, reverses the ethanolamine auxotrophy. However, the phosphatidylethanolamine content is not normalized unless the culture medium is supplemented with ethanolamine. In both mutant chinese hamster ovary cells and hepatoma cells transfected with PSS2 cDNA the rate of synthesis of phosphatidylserine and phosphatidylserine-derived phosphatidylethanolamine does not exceed that in parental chinese hamster ovary cells or control McArdle cells, respectively. Expression of murine PSS2 in McArdle cells does not inhibit phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway, whereas expression of similar levels of PSS1 activity inhibit this pathway by approx. 50%
Mus musculus
Other publictions for EC 2.7.8.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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693046
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Defining the importance of pho ...
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J. Biol. Chem.
283
12888-12897
2008
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J. Neurosci. Res.
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1568-1578
2007
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2007
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Biochim. Biophys. Acta
1636
1-11
2004
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717768
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Purification and characterizat ...
Cricetulus griseus
J. Biol. Chem.
278
42692-42698
2003
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717766
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Defining the importance of pho ...
Mus musculus
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277
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2002
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717762
Stone
Phosphatidylserine synthase-1 ...
Cricetulus griseus, Rattus norvegicus
J. Biol. Chem.
275
34534-34540
2000
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717208
Stone
Cloning and expression of muri ...
Mus musculus
Biochem. J.
342
57-64
1999
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717761
Kuge
Control of phosphatidylserine ...
Cricetulus griseus
J. Biol. Chem.
274
23844-23849
1999
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717759
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Genetic evidence that phosphat ...
Cricetulus griseus
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273
17199-17205
1998
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717758
Kuge
Cloning of a Chinese hamster o ...
Cricetulus griseus
J. Biol. Chem.
272
19133-19139
1997
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