BRENDA - Enzyme Database show
show all sequences of 2.7.8.23

Cloning, overexpression and mechanistic studies of carboxyphosphoenolpyruvate mutase from Streptomyces hygroscopicus

Pollack, S.J.; Freeman, S.; Pompliano, D.L.; Knowles, F.R.; Eur. J. Biochem. 209, 735-743 (1992)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
Streptomyces hygroscopicus
Cloned(Commentary)
Commentary
Organism
gene cloned from a subgenomic library cloned by colony hybridisation in Escherichia coli AG1, subcloned into vector pET11a and overexpressed in Escherichia coli BL21(DE3)
Streptomyces hygroscopicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0022
-
carboxyphosphinopyruvate
pH 6.5, 30°C
Streptomyces hygroscopicus
0.27
-
carboxyphosphoenolpyruvate
pH 6.5, 30°C
Streptomyces hygroscopicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
reaction shows metal dependence
Streptomyces hygroscopicus
Mn2+
increases activity, about 10times more active compared with the activity in presence of Mg2+
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
no activity in Streptomyces hygroscopicus
-
-
-
Streptomyces hygroscopicus
-
ATCC 21705
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces hygroscopicus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activity 37.6 U/mg x 1000
Streptomyces hygroscopicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carboxyphosphinopyruvate
-
643773
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643773
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
-
643773
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643773
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
643773
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643773
Streptomyces hygroscopicus
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00076
-
carboxyphosphinopyruvate
pH 7.5, 30°C
Streptomyces hygroscopicus
0.02
-
carboxyphosphoenolpyruvate
pH 7.5, 30°C
Streptomyces hygroscopicus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
8
stable in this range
Streptomyces hygroscopicus
Application (protein specific)
Application
Commentary
Organism
agriculture
formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
Streptomyces hygroscopicus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene cloned from a subgenomic library cloned by colony hybridisation in Escherichia coli AG1, subcloned into vector pET11a and overexpressed in Escherichia coli BL21(DE3)
Streptomyces hygroscopicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0022
-
carboxyphosphinopyruvate
pH 6.5, 30°C
Streptomyces hygroscopicus
0.27
-
carboxyphosphoenolpyruvate
pH 6.5, 30°C
Streptomyces hygroscopicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
reaction shows metal dependence
Streptomyces hygroscopicus
Mn2+
increases activity, about 10times more active compared with the activity in presence of Mg2+
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces hygroscopicus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activity 37.6 U/mg x 1000
Streptomyces hygroscopicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carboxyphosphinopyruvate
-
643773
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643773
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
-
643773
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643773
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
643773
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643773
Streptomyces hygroscopicus
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00076
-
carboxyphosphinopyruvate
pH 7.5, 30°C
Streptomyces hygroscopicus
0.02
-
carboxyphosphoenolpyruvate
pH 7.5, 30°C
Streptomyces hygroscopicus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
8
stable in this range
Streptomyces hygroscopicus
Other publictions for EC 2.7.8.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723443
Chen
-
Identification of a co-segrega ...
Oryza sativa
Plant OMICS
6
36-45
2013
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
1
1
1
1
1
-
-
643771
Freeman
-
Synthesis of the unusual metab ...
Streptomyces hygroscopicus
J. Am. Chem. Soc.
114
377-378
1992
-
1
1
-
-
-
-
1
-
-
2
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
2
1
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
643772
Hidaka
Nucleotide sequence of a carbo ...
Streptomyces hygroscopicus
Mol. Gen. Genet.
233
476-478
1992
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643773
Pollack
Cloning, overexpression and me ...
no activity in Streptomyces hygroscopicus, Streptomyces hygroscopicus
Eur. J. Biochem.
209
735-743
1992
-
1
1
-
-
-
-
2
-
2
-
1
-
2
-
-
1
-
-
-
1
-
3
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
643768
Hidaka
Biochemical mechanism of C-P b ...
no activity in Streptomyces hygroscopicus, Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
Agric. Biol. Chem.
54
2121-2125
1990
-
1
1
-
-
-
-
-
-
-
-
4
-
7
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643769
Hidaka
Carboxyphosphoenolpyruvate pho ...
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
J. Bacteriol.
172
3066-3072
1990
-
1
1
-
-
-
1
1
-
3
2
2
-
7
-
-
1
-
-
1
1
2
4
1
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
1
-
1
-
3
2
2
-
-
-
1
-
1
1
2
4
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
643770
Hidaka
-
Comparison of two C-P bond-for ...
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
Agric. Biol. Chem.
54
2467-2468
1990
-
-
-
-
-
-
2
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-