BRENDA - Enzyme Database
show all sequences of 2.7.8.23

Synthesis of the unusual metabolite carboxyphosphoenolpyruvate. Cloning and expression of carboxyphosphonoenolpyruvate mutase

Freeman, S.; Pollack, S.J.; Knowles, J.R.; J. Am. Chem. Soc. 114, 377-378 (1992)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
Streptomyces hygroscopicus
Cloned(Commentary)
Cloned (Commentary)
Organism
cloned and expressd at high levels in Escherichia coli
Streptomyces hygroscopicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.27
-
carboxyphosphoenolpyruvate
-
Streptomyces hygroscopicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, homodimer, SDS-PAGE
Streptomyces hygroscopicus
32700
-
calculated from amino acid composition
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus
-
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces hygroscopicus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Streptomyces hygroscopicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
carboxyphosphonoenolpyruvate
-
643771
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
-
643771
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643771
Streptomyces hygroscopicus
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 32000, homodimer, SDS-PAGE
Streptomyces hygroscopicus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.02
-
carboxyphosphoenolpyruvate
-
Streptomyces hygroscopicus
Application (protein specific)
Application
Commentary
Organism
agriculture
formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
Streptomyces hygroscopicus
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned and expressd at high levels in Escherichia coli
Streptomyces hygroscopicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.27
-
carboxyphosphoenolpyruvate
-
Streptomyces hygroscopicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, homodimer, SDS-PAGE
Streptomyces hygroscopicus
32700
-
calculated from amino acid composition
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus
-
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces hygroscopicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
carboxyphosphonoenolpyruvate
-
643771
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
-
643771
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643771
Streptomyces hygroscopicus
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 32000, homodimer, SDS-PAGE
Streptomyces hygroscopicus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.02
-
carboxyphosphoenolpyruvate
-
Streptomyces hygroscopicus
Other publictions for EC 2.7.8.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723443
Chen
-
Identification of a co-segrega ...
Oryza sativa
Plant OMICS
6
36-45
2013
-
-
-
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-
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-
-
-
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1
1
-
1
-
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1
-
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2
1
3
-
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-
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1
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1
1
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-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
1
1
1
1
1
-
-
643771
Freeman
-
Synthesis of the unusual metab ...
Streptomyces hygroscopicus
J. Am. Chem. Soc.
114
377-378
1992
-
1
1
-
-
-
-
1
-
-
2
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
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-
1
1
-
-
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-
-
-
1
-
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2
1
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
643772
Hidaka
Nucleotide sequence of a carbo ...
Streptomyces hygroscopicus
Mol. Gen. Genet.
233
476-478
1992
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
-
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-
2
1
-
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-
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-
1
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-
-
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2
1
-
-
-
1
-
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-
2
1
-
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-
-
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-
-
-
-
-
-
-
-
643773
Pollack
Cloning, overexpression and me ...
no activity in Streptomyces hygroscopicus, Streptomyces hygroscopicus
Eur. J. Biochem.
209
735-743
1992
-
1
1
-
-
-
-
2
-
2
-
1
-
2
-
-
1
-
-
-
1
-
3
-
1
-
-
-
2
-
-
1
-
-
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-
1
1
-
-
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-
-
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-
2
-
2
-
1
-
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1
-
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1
-
3
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
643768
Hidaka
Biochemical mechanism of C-P b ...
no activity in Streptomyces hygroscopicus, Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
Agric. Biol. Chem.
54
2121-2125
1990
-
1
1
-
-
-
-
-
-
-
-
4
-
7
-
-
-
-
-
-
-
-
6
-
-
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-
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-
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-
1
1
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4
-
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6
-
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643769
Hidaka
Carboxyphosphoenolpyruvate pho ...
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
J. Bacteriol.
172
3066-3072
1990
-
1
1
-
-
-
1
1
-
3
2
2
-
7
-
-
1
-
-
1
1
2
4
1
-
1
-
-
-
1
-
1
-
-
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-
-
1
1
-
-
-
-
-
1
-
1
-
3
2
2
-
-
-
1
-
1
1
2
4
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
643770
Hidaka
-
Comparison of two C-P bond-for ...
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
Agric. Biol. Chem.
54
2467-2468
1990
-
-
-
-
-
-
2
-
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-
-
2
-
6
-
-
-
-
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-
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4
-
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2
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2
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4
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