BRENDA - Enzyme Database show
show all sequences of 2.7.8.23

Biochemical mechanism of C-P bond formation of bialaphos: use of gene manipulation for the analysis of the C-P bond formation step

Hidaka, T.; Hara, O.; Imai, S.; Anzai, H.; Murakami, T.; Nakaoka, K.; Seto, H.; Agric. Biol. Chem. 54, 2121-2125 (1990)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
Streptomyces hygroscopicus
Cloned(Commentary)
Commentary
Organism
mutant NP71 constructed by gene replacement technique
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus
first natural phosphinate
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus SF1293
first natural phosphinate
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus SF1293
?
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus SF1293
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus SF1293
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
no activity in Streptomyces hygroscopicus
-
-
-
Streptomyces hygroscopicus
-
HP782 and mutant NP71; SF1293
-
Streptomyces hygroscopicus SF1293
-
SF1293
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carboxyphosphonoenolpyruvate
-
643768
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
first natural phosphinate
643768
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643768
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
643768
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643768
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
-
643768
Streptomyces hygroscopicus SF1293
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
first natural phosphinate
643768
Streptomyces hygroscopicus SF1293
(hydroxyphosphinyl)pyruvate + CO2
-
643768
Streptomyces hygroscopicus SF1293
?
carboxyphosphonoenolpyruvate
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
643768
Streptomyces hygroscopicus SF1293
(hydroxyphosphinyl)pyruvate + CO2
-
643768
Streptomyces hygroscopicus SF1293
?
Application (protein specific)
Application
Commentary
Organism
agriculture
formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
Streptomyces hygroscopicus
Cloned(Commentary) (protein specific)
Commentary
Organism
mutant NP71 constructed by gene replacement technique
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus
first natural phosphinate
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus SF1293
first natural phosphinate
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus SF1293
?
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus SF1293
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus SF1293
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carboxyphosphonoenolpyruvate
-
643768
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
first natural phosphinate
643768
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643768
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
643768
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643768
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
-
643768
Streptomyces hygroscopicus SF1293
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
first natural phosphinate
643768
Streptomyces hygroscopicus SF1293
(hydroxyphosphinyl)pyruvate + CO2
-
643768
Streptomyces hygroscopicus SF1293
?
carboxyphosphonoenolpyruvate
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
643768
Streptomyces hygroscopicus SF1293
(hydroxyphosphinyl)pyruvate + CO2
-
643768
Streptomyces hygroscopicus SF1293
?
Other publictions for EC 2.7.8.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723443
Chen
-
Identification of a co-segrega ...
Oryza sativa
Plant OMICS
6
36-45
2013
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1
1
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1
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1
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2
1
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1
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1
1
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-
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-
1
-
-
2
1
-
-
-
-
-
-
-
1
1
1
1
1
-
-
643771
Freeman
-
Synthesis of the unusual metab ...
Streptomyces hygroscopicus
J. Am. Chem. Soc.
114
377-378
1992
-
1
1
-
-
-
-
1
-
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2
1
-
1
-
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1
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2
1
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1
-
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1
1
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1
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2
1
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1
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2
1
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1
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-
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-
-
-
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643772
Hidaka
Nucleotide sequence of a carbo ...
Streptomyces hygroscopicus
Mol. Gen. Genet.
233
476-478
1992
-
-
1
-
-
-
-
-
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2
1
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3
-
-
1
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2
1
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1
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2
1
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1
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2
1
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-
-
-
-
643773
Pollack
Cloning, overexpression and me ...
no activity in Streptomyces hygroscopicus, Streptomyces hygroscopicus
Eur. J. Biochem.
209
735-743
1992
-
1
1
-
-
-
-
2
-
2
-
1
-
2
-
-
1
-
-
-
1
-
3
-
-
-
-
2
-
-
1
-
-
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1
1
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-
-
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-
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2
-
2
-
1
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1
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-
1
-
3
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
643768
Hidaka
Biochemical mechanism of C-P b ...
no activity in Streptomyces hygroscopicus, Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
Agric. Biol. Chem.
54
2121-2125
1990
-
1
1
-
-
-
-
-
-
-
-
4
-
7
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
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-
-
1
1
-
-
-
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-
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4
-
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6
-
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-
643769
Hidaka
Carboxyphosphoenolpyruvate pho ...
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
J. Bacteriol.
172
3066-3072
1990
-
1
1
-
-
-
1
1
-
3
2
2
-
7
-
-
1
-
-
1
1
2
4
1
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
1
-
1
-
3
2
2
-
-
-
1
-
1
1
2
4
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
643770
Hidaka
-
Comparison of two C-P bond-for ...
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
Agric. Biol. Chem.
54
2467-2468
1990
-
-
-
-
-
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2
-
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2
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6
-
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4
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2
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2
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4
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