BRENDA - Enzyme Database
show all sequences of 2.7.8.23

Carboxyphosphoenolpyruvate phosphonomutase, a novel enzyme catalyzing C-P bond formation

Hidaka, T.; Imai, S.; Hara, O.; Anzai, H.; Murakami, T.; Nagaoka, K.; Seto, H.; J. Bacteriol. 172, 3066-3072 (1990)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
Streptomyces hygroscopicus
Cloned(Commentary)
Commentary
Organism
mutant NP71 constructed by gene replacement technique
Streptomyces hygroscopicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
enzyme is inactive in presence of EDTA, but inhibition is completely relieved by addition of 1 mM MnSO4 or MgSO4
Streptomyces hygroscopicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
carboxyphosphoenolpyruvate
pH 6.5, 27°C
Streptomyces hygroscopicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
increases activity, 1-10 mM MgSO4, relative activity 250%
Streptomyces hygroscopicus
Mn2+
1 mM MnSO4, relative activity 680%, 10 mM MnSO4, relative activity 600%
Streptomyces hygroscopicus
additional information
NaCl, KCl and NH4(SO4)2 up to 100 mM have no effect
Streptomyces hygroscopicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, homodimer, SDS-PAGE
Streptomyces hygroscopicus
56000
-
gel filtration
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus SF1293
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus SF1293
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces hygroscopicus
-
HP782, HP5-29 and SF1293 mutant NP71; SF1293
-
Streptomyces hygroscopicus SF1293
-
SF1293
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces hygroscopicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Streptomyces hygroscopicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activity 217.0 U/mg of protein, one unit is the amount of enzyme required to produce 1micromol of bialaphos
Streptomyces hygroscopicus
Storage Stability
Storage Stability
Organism
-20°C, 80% ammonium sulfate suspension, no loss of activity observed for 1 month
Streptomyces hygroscopicus
4°C, 1 mg/ml in 50 mM MES buffer, pH 6.5, stable for 1 week
Streptomyces hygroscopicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carboxyphosphonoenolpyruvate
catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate
643769
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
643769
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643769
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate
643769
Streptomyces hygroscopicus SF1293
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
643769
Streptomyces hygroscopicus SF1293
(hydroxyphosphinyl)pyruvate + CO2
-
643769
Streptomyces hygroscopicus SF1293
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 32000, homodimer, SDS-PAGE
Streptomyces hygroscopicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Streptomyces hygroscopicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Streptomyces hygroscopicus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
8
stable in this range
Streptomyces hygroscopicus
Application (protein specific)
Application
Commentary
Organism
agriculture
formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
Streptomyces hygroscopicus
Cloned(Commentary) (protein specific)
Commentary
Organism
mutant NP71 constructed by gene replacement technique
Streptomyces hygroscopicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
enzyme is inactive in presence of EDTA, but inhibition is completely relieved by addition of 1 mM MnSO4 or MgSO4
Streptomyces hygroscopicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
carboxyphosphoenolpyruvate
pH 6.5, 27°C
Streptomyces hygroscopicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
increases activity, 1-10 mM MgSO4, relative activity 250%
Streptomyces hygroscopicus
Mn2+
1 mM MnSO4, relative activity 680%, 10 mM MnSO4, relative activity 600%
Streptomyces hygroscopicus
additional information
NaCl, KCl and NH4(SO4)2 up to 100 mM have no effect
Streptomyces hygroscopicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, homodimer, SDS-PAGE
Streptomyces hygroscopicus
56000
-
gel filtration
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
Streptomyces hygroscopicus SF1293
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
(hydroxyphosphinyl)pyruvate + CO2
-
Streptomyces hygroscopicus SF1293
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces hygroscopicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Streptomyces hygroscopicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activity 217.0 U/mg of protein, one unit is the amount of enzyme required to produce 1micromol of bialaphos
Streptomyces hygroscopicus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 80% ammonium sulfate suspension, no loss of activity observed for 1 month
Streptomyces hygroscopicus
4°C, 1 mg/ml in 50 mM MES buffer, pH 6.5, stable for 1 week
Streptomyces hygroscopicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carboxyphosphonoenolpyruvate
catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate
643769
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
643769
Streptomyces hygroscopicus
(hydroxyphosphinyl)pyruvate + CO2
-
643769
Streptomyces hygroscopicus
?
carboxyphosphonoenolpyruvate
catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate
643769
Streptomyces hygroscopicus SF1293
(hydroxyphosphinyl)pyruvate + CO2
-
-
-
?
carboxyphosphonoenolpyruvate
biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos
643769
Streptomyces hygroscopicus SF1293
(hydroxyphosphinyl)pyruvate + CO2
-
643769
Streptomyces hygroscopicus SF1293
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 32000, homodimer, SDS-PAGE
Streptomyces hygroscopicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Streptomyces hygroscopicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Streptomyces hygroscopicus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
8
stable in this range
Streptomyces hygroscopicus
Other publictions for EC 2.7.8.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723443
Chen
-
Identification of a co-segrega ...
Oryza sativa
Plant OMICS
6
36-45
2013
-
-
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1
1
-
1
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-
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1
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2
1
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-
-
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1
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-
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-
1
1
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-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
1
1
1
1
1
-
-
643771
Freeman
-
Synthesis of the unusual metab ...
Streptomyces hygroscopicus
J. Am. Chem. Soc.
114
377-378
1992
-
1
1
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1
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2
1
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1
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1
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2
1
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1
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1
1
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1
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2
1
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1
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2
1
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1
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-
-
-
643772
Hidaka
Nucleotide sequence of a carbo ...
Streptomyces hygroscopicus
Mol. Gen. Genet.
233
476-478
1992
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
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1
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2
1
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1
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2
1
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1
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2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643773
Pollack
Cloning, overexpression and me ...
no activity in Streptomyces hygroscopicus, Streptomyces hygroscopicus
Eur. J. Biochem.
209
735-743
1992
-
1
1
-
-
-
-
2
-
2
-
1
-
2
-
-
1
-
-
-
1
-
3
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
643768
Hidaka
Biochemical mechanism of C-P b ...
no activity in Streptomyces hygroscopicus, Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
Agric. Biol. Chem.
54
2121-2125
1990
-
1
1
-
-
-
-
-
-
-
-
4
-
7
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643769
Hidaka
Carboxyphosphoenolpyruvate pho ...
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
J. Bacteriol.
172
3066-3072
1990
-
1
1
-
-
-
1
1
-
3
2
2
-
7
-
-
1
-
-
1
1
2
4
1
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
1
-
1
-
3
2
2
-
-
-
1
-
1
1
2
4
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
643770
Hidaka
-
Comparison of two C-P bond-for ...
Streptomyces hygroscopicus, Streptomyces hygroscopicus SF1293
Agric. Biol. Chem.
54
2467-2468
1990
-
-
-
-
-
-
2
-
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2
-
6
-
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4
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2
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2
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4
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