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Literature summary for 2.7.8.21 extracted from
- Biosynthesis of membrane-derived oligosaccharides: a periplasmic phosphoglyceroltransferase (1981), Proc. Natl. Acad. Sci. USA, 78, 5513-5517.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | 5 mM, competitive to membrane-derived oligosaccharides, 77% of the activity without addition | Escherichia coli |  |
| D-glucose | 5 mM, competitive to membrane-derived oligosaccharides, 78% of the activity without addition | Escherichia coli | |
| D-maltose | 5 mM, competitive to membrane-derived oligosaccharides, 82% of the activity without addition | Escherichia coli | |
| EDTA | - |
Escherichia coli | |
| gentiobiose | 5 mM, competitive to membrane-derived oligosaccharides, 15% of the activity without addition | Escherichia coli | |
| sophorose | 5 mM, competitive to membrane-derived oligosaccharides, 56% of the activity without addition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
gentiobiose | pH 7.8, 37°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasmic space | - |
Escherichia coli | 42597 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | less effective than Mn2+ | Escherichia coli | |
| Co2+ | less effective than Mn2+ | Escherichia coli | |
| Mg2+ | less effective than Mn2+ | Escherichia coli | |
| Mn2+ | absolute requirement for a divalent cation, Mn2+ most active | Escherichia coli | |
| additional information | absolute requirement for a divalent cation | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| membrane-derived oligosaccharides | Escherichia coli | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-gentiobiose | - |
Escherichia coli | ? | - |
? | |
| membrane-derived oligosaccharides | transfer of a glycerophospho group from one membrane-derived oligosaccharide to another. beta-Linked glucose residues in simple glucosides, such as gentiobiose, can act as acceptors | Escherichia coli | additional information | in presence of low concentrations of acceptor, free cyclic 1,2-phosphoglycerol is formed | ? | |
| membrane-derived oligosaccharides | - |
Escherichia coli | ? | - |
? | |
| additional information | no activity detected with phosphatidylglycerol as donor, or suspensions of phospholipid mixed with Triton X-100 or in mixed vesicles with phosphatidylcholine | Escherichia coli | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.8 | active | Escherichia coli |
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Release 2023.1 (January 2023)
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