Cloned (Comment) | Organism |
---|---|
gene PDCT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, the gene sequences of isozymes LuPDCT1 and LuPDCT2 show very high nucleotide sequence identity of 97%, functional complementation of the enzyme-deficient rod1 mutant of Arabidopsis thaliana by expression of the seed-speficic isozymes, restoring polyunsaturated fatty acid levels (16.4% and 19.7% increases in C18-PUFAs, respectively, with a concomitant decrease in the proportion of oleic acid). Functional coexpression of the isozymes with fatty acid desaturases FAD2 and FAD3 in Saccharomyces cerevisiae leads to increased polyunsaturated fatty acid levels | Linum usitatissimum |
gene PDCT2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, the gene sequences of isozymes LuPDCT1 and LuPDCT2 show very high nucleotide sequence identity of 97%, functional complementation of the enzyme-deficient rod1 mutant of Arabidopsis thaliana by expression of the seed-speficic isozymes, restoring polyunsaturated fatty acid levels (16.4% and 19.7% increases in C18-PUFAs, respectively, with a concomitant decrease in the proportion of oleic acid). Functional coexpression of the isozymes with fatty acid desaturases FAD2 and FAD3 in Saccharomyces cerevisiae leads to increased polyunsaturated fatty acid levels | Linum usitatissimum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-choline + 1,2-diacyl-sn-glycerol | Linum usitatissimum | enzyme phosphatidylcholine diacylglycerol cholinephosphotransferase catalyzes the interconversion between phosphatidylcholine and diacylglycerol | CMP + a phosphatidylcholine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Linum usitatissimum | A0A0H3U1R3 | PDCT1 | - |
Linum usitatissimum | A0A0H3U1R4 | PDCT2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | developing, the isozymes are both highly expressed during the mid to late stages, 8 to 16 days after anthesis, DAA, of embryo development, peaking at approximately 11 DAA with transcript levels decreasing dramatically after 16 DAA to nearly undetectable levels for the remainder of seed development | Linum usitatissimum | - |
additional information | isozymes LuPDCT1 and LuPDCT2 exhibit similar spatiotemporal patterns of expression. Both LuPDCT1 and LuPDCT2 are expressed at very low levels in all vegetative and floral tissues tested, stem, leaves, shoot apex, root and flowers | Linum usitatissimum | - |
seed | - |
Linum usitatissimum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-choline + 1,2-diacyl-sn-glycerol | - |
Linum usitatissimum | CMP + a phosphatidylcholine | - |
r | |
CDP-choline + 1,2-diacyl-sn-glycerol | enzyme phosphatidylcholine diacylglycerol cholinephosphotransferase catalyzes the interconversion between phosphatidylcholine and diacylglycerol | Linum usitatissimum | CMP + a phosphatidylcholine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PDCT | - |
Linum usitatissimum |
PDCT1 | - |
Linum usitatissimum |
PDCT2 | - |
Linum usitatissimum |
phosphatidylcholine diacylglycerol cholinephosphotransferase | - |
Linum usitatissimum |
phospholipid:diacylglycerol choline phosphotransferase | - |
Linum usitatissimum |