Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.8.2 extracted from

  • Wickramarathna, A.; Siloto, R.; Mietkiewska, E.; Singer, S.; Pan, X.; Weselake, R.
    Heterologous expression of flax phospholipid:diacylglycerol choline phosphotransferase (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds (2015), BMC Biotechnol., 15, 63.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene PDCT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, the gene sequences of isozymes LuPDCT1 and LuPDCT2 show very high nucleotide sequence identity of 97%, functional complementation of the enzyme-deficient rod1 mutant of Arabidopsis thaliana by expression of the seed-speficic isozymes, restoring polyunsaturated fatty acid levels (16.4% and 19.7% increases in C18-PUFAs, respectively, with a concomitant decrease in the proportion of oleic acid). Functional coexpression of the isozymes with fatty acid desaturases FAD2 and FAD3 in Saccharomyces cerevisiae leads to increased polyunsaturated fatty acid levels Linum usitatissimum
gene PDCT2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, the gene sequences of isozymes LuPDCT1 and LuPDCT2 show very high nucleotide sequence identity of 97%, functional complementation of the enzyme-deficient rod1 mutant of Arabidopsis thaliana by expression of the seed-speficic isozymes, restoring polyunsaturated fatty acid levels (16.4% and 19.7% increases in C18-PUFAs, respectively, with a concomitant decrease in the proportion of oleic acid). Functional coexpression of the isozymes with fatty acid desaturases FAD2 and FAD3 in Saccharomyces cerevisiae leads to increased polyunsaturated fatty acid levels Linum usitatissimum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CDP-choline + 1,2-diacyl-sn-glycerol Linum usitatissimum enzyme phosphatidylcholine diacylglycerol cholinephosphotransferase catalyzes the interconversion between phosphatidylcholine and diacylglycerol CMP + a phosphatidylcholine
-
r

Organism

Organism UniProt Comment Textmining
Linum usitatissimum A0A0H3U1R3 PDCT1
-
Linum usitatissimum A0A0H3U1R4 PDCT2
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo developing, the isozymes are both highly expressed during the mid to late stages, 8 to 16 days after anthesis, DAA, of embryo development, peaking at approximately 11 DAA with transcript levels decreasing dramatically after 16 DAA to nearly undetectable levels for the remainder of seed development Linum usitatissimum
-
additional information isozymes LuPDCT1 and LuPDCT2 exhibit similar spatiotemporal patterns of expression. Both LuPDCT1 and LuPDCT2 are expressed at very low levels in all vegetative and floral tissues tested, stem, leaves, shoot apex, root and flowers Linum usitatissimum
-
seed
-
Linum usitatissimum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-choline + 1,2-diacyl-sn-glycerol
-
Linum usitatissimum CMP + a phosphatidylcholine
-
r
CDP-choline + 1,2-diacyl-sn-glycerol enzyme phosphatidylcholine diacylglycerol cholinephosphotransferase catalyzes the interconversion between phosphatidylcholine and diacylglycerol Linum usitatissimum CMP + a phosphatidylcholine
-
r

Synonyms

Synonyms Comment Organism
PDCT
-
Linum usitatissimum
PDCT1
-
Linum usitatissimum
PDCT2
-
Linum usitatissimum
phosphatidylcholine diacylglycerol cholinephosphotransferase
-
Linum usitatissimum
phospholipid:diacylglycerol choline phosphotransferase
-
Linum usitatissimum