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Literature summary for 2.7.8.2 extracted from

  • Tsoupras, A.B.; Fragopoulou, E.; Nomikos, T.; Iatrou, C.; Antonopoulou, S.; Demopoulos, C.A.
    Characterization of the de novo biosynthetic enzyme of platelet activating factor, DDT-insensitive cholinephosphotransferase, of human mesangial cells (2007), Mediators Inflamm., 2007, 27683.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.2

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin specific activity increases in presence of 0.5-2 mg/ml bovine serum albumin, microsomal fraction of human mesangial cells Homo sapiens
dithiothreitol specific activity slightly increases at dithiothreitol (DTT) concentrations above 1 mM, optimum stimulation at 15 mM DTT, microsomal fraction of human mesangial cells Homo sapiens
EDTA maximum activation at 0.5 mM, in presence of 20 mM Mg2+ Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 0.1 mM (14.1% activity left) or 1 mM (1.41% activity left), estimated in presence of 20 mM Mg2+, inhibition partially reduced in presence of 0.5 mM EDTA: 33.3% or 3.70% activity left for 0.1 mM or 1 mM Ca2+, respectively (100% activity: 20 mM Mg2+, 0.5 mM EDTA, no Ca2+) Homo sapiens
EDTA 10 mM, in presence of 20 mM Mg2+ Homo sapiens
INFalpha 250 inhibitory units/microl, 51% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
NaF sodium fluoride, 50 mM, 40% reduction in activity Homo sapiens
polar lipids from olive oil 5.2 ng/microl, 33% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
polar lipids from olive pomace 1.8 ng/microl, 48% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
polar lipids from sea bass 59 ng/microl, 45% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
polar lipids from sea bream 123 ng/microl, 17% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
resveratrol 100 microM, 39% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
rupatadine 20 ng/microl, 60% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
salicylic acid 250 ng/microl, 38% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
simvastatine 40 ng/microl, 92% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
tinzaparin 0.25 inhibitory units/microl, 30% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0166
-
 CDP-choline optimum reaction conditions, in presence of 100 microM 1-O-alkyl-2-acetyl-sn-glycerol Homo sapiens
0.0593
-
 1-O-alkyl-2-acetyl-sn-glycerol optimum reaction conditions, in presence of 100 microM CDP-choline Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome highest activity in microsomal fraction of mesangial cells Homo sapiens
-
-
additional information no activity detectable in cytoplasmic fraction of mesangial cells Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 20 mM, maximum activation in presence of 0.5 mM EDTA Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CDP-choline + 1-O-alkyl-2-acetyl-sn-glycerol Homo sapiens platelet activating factor (PAF) de novo biosynthesis CMP + 1-O-alkyl-2-acetyl-sn-glycerol 3-phosphocholine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mesangial cell HMC, immortilized by transfection with T-antigen of SV40 and Hras oncogene Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 linear relationship between initial velocity and protein concentration in microsomal fraction of human mesangial cells up to 0.1 mg/ml Homo sapiens
additional information
-
 Pefabloc has no affect on enzyme activity, microsomal fraction of human mesangial cells Homo sapiens
0.0000003
-
 0.01 mM Mg2+, microsomal fraction of human mesangial cells Homo sapiens
0.0000012
-
 1 mM Mg2+, microsomal fraction of human mesangial cells Homo sapiens
0.0000036
-
 10 mM Mg2+, microsomal fraction of human mesangial cells Homo sapiens
0.00002
-
 mitochondrial fraction of human mesangial cells Homo sapiens
0.000067
-
 20 mM Mg2+, microsomal fraction of human mesangial cells Homo sapiens
0.0001
-
 homogenate of human mesangial cells Homo sapiens
0.00025
-
 20 mM Mg2+, 0.5 mM EDTA, microsomal fraction of human mesangial cells Homo sapiens
0.00036
-
 microsomal fraction of human mesangial cells, maximum activity in presence of 1 mg/ml bovine serum albumin, 15 mM dithiothreitol, 20 mM Mg2+, 0.5 mM EDTA Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, microsomal fraction of human mesangial cells (HMC), 30 days Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-choline + 1-O-alkyl-2-acetyl-sn-glycerol platelet activating factor (PAF) de novo biosynthesis Homo sapiens CMP + 1-O-alkyl-2-acetyl-sn-glycerol 3-phosphocholine
-
 ?
CDP-choline + 1-O-alkyl-2-acetyl-sn-glycerol 37°C, 20 min, pH 8, in presence of 15 mM dithiothreitol, 5 mM EDTA, 20 mM MgCl2, 1 mg/ml bovine serum albumin Homo sapiens CMP + 1-O-alkyl-2-acetyl-sn-glycerol 3-phosphocholine analysis by thin-layer chromatography ?

Synonyms

Synonyms Comment Organism
DTT-insensitive CDP-choline: 1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase
-
 Homo sapiens
DTT-insensitive cholinephosphotransferase DTT: dithiothreitol Homo sapiens
PAF-CPT PAF: platelet activating factor, to be distinguished from PC-CPT, PC: phosphatidylcholine Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 microsomal fraction of human mesangial cells (HMC), bell-shaped temperature activity profile Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 20 min heating results in loss of 80% of activity, microsomal fraction of human mesangial cells (HMC) Homo sapiens
60
-
 20 min heating results in total loss of activity, microsomal fraction of human mesangial cells (HMC) Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 100 mM Tris-HCl, bell-shaped pH activity profile Homo sapiens