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Literature summary for 2.7.8.2 extracted from
- Solubilization and reconstitution of cholinephosphotransferase from sarcoplasmic reticulum: stabilization of solubilized enzyme by diacylglycerol and glycerol (1985), Biochim. Biophys. Acta, 821, 97-105.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cholate | inactivation at solubilization concentration, full recovery of activity after reconstituting the membrane by adding excess lipid and removing detergent by gel filtration, dialysis or absorption to Bio-Beads, if membrane is solubilized with octylglucoside or cholate at weight ratios of detergent:membrane protein of at least 10, the activity is irreversibly lost unless stabilizers are added with detergent, diacylglycerol and glycerol are effective stabilizers | Oryctolagus cuniculus |  |
| n-octylglucoside | inactivation at solubilization concentration, full recovery of activity after reconstituting the membrane by adding excess lipid and removing detergent by gel filtration, dialysis or absorption to Bio-Beads, if membrane is solubilized with octylglucoside or cholate at weight ratios of detergent:membrane protein of at least 10, the activity is irreversibly lost unless stabilizers are added with detergent, diacylglycerol and glycerol are effective stabilizers | Oryctolagus cuniculus | |
| SDS | inactivation at solubilization concentration, full recovery of activity after reconstituting the membrane by adding excess lipid and removing detergent by gel filtration, dialysis or absorption to Bio-Beads | Oryctolagus cuniculus | |
| Triton X-100 | inactivation at solubilization concentration, full recovery of activity after reconstituting the membrane by adding excess lipid (soybean) and removing detergent by gel filtration, dialysis or absorption to Bio-Beads; X-100 | Oryctolagus cuniculus | |
| Tween-20 | inactivation at solubilization concentration, full recovery of activity after reconstituting the membrane by adding excess lipid (soybean) and removing detergent by gel filtration, dialysis or absorption to Bio-Beads | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| sarcoplasmic reticulum | - |
Oryctolagus cuniculus | 16529 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-choline + sn-1,2-diacylglycerol | Oryctolagus cuniculus | final reaction in synthesis of phosphatidylcholine | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-choline + 1,2-diacylglycerol | - |
Oryctolagus cuniculus | CMP + a phosphatidylcholine | - |
? | |
| CDP-choline + sn-1,2-diacylglycerol | final reaction in synthesis of phosphatidylcholine | Oryctolagus cuniculus | ? | - |
? | |
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