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Literature summary for 2.7.8.2 extracted from

  • Bru, R.; Blöchliger, E.; Luisi, P.L.
    sn-1,2-Diacylglycerol cholinephosphotransferase from pig liver: mixed micellar assay and kinetic analysis of the partially pure enzyme (1993), Arch. Biochem. Biophys., 307, 295-303.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dioleoylphosphatidylcholine stimulates Sus scrofa
additional information cholinephosphotransferase requires a lipidic boundary for full activation, no activation by substrate Sus scrofa
phosphatidylcholine activates Sus scrofa

General Stability

General Stability Organism
soy phosphatidylcholine protects against detergent inactivation Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Sus scrofa
-
-

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0132
-
30°C, pH 7.4 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-choline + 1,2-diacylglycerol
-
Sus scrofa CMP + a phosphatidylcholine
-
?
CDP-choline + didecanoylglycerol at 5-10% of the activity with dioleoylglycerol or egg diglycerides Sus scrofa CMP + didecanoylglycerophosphocholine
-
?
CDP-choline + dihexanoylglycerol at 5-10% of the activity with dioleoylglycerol or egg diglycerides Sus scrofa CMP + dihexanoylglycerophosphocholine
-
?
CDP-choline + dioctanoylglycerol at 5-10% of the activity with dioleoylglycerol or egg diglycerides Sus scrofa CMP + dioctanoylglycerophosphocholine
-
?
additional information utilizes 1-myristyl phosphatidylcholine most rapidly and, in decreasing order the 1-palmitoyl and 1-stearyl species Sus scrofa ?
-
?
additional information utilizes without marked selectivity the endogenous 1,2-diacylglycerol species differing in the degree of unsaturation Sus scrofa ?
-
?