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Literature summary for 2.7.8.17 extracted from

  • Qian, Y.; Lee, I.; Lee, W.; Qian, M.; Kudo, M.; Canfield, W.; Lobel, P.; Kornfeld, S.
    Functions of the alpha, beta, gamma subunits of UDP-GlcNAc:Lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (2010), J. Biol. Chem., 285, 3360-3370.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.17

Cloned(Commentary)

Cloned (Comment) Organism
alpha2beta2gamma2 and alpha2beta2 GlcNAc-1-phosphotransferase are stably expressed in CHO-K1 cells (transmembrane segments are missing) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cathepsin D deglycosylated cathepsin D inhibits the activity of both forms of GlcNAc-1-phosphotransferase toward cathepsin D Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 uteroferrin pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.025
-
 cathepsin D pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.025
-
 cathepsin D pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.03
-
 DNase I pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.033
-
 uteroferrin pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.06
-
 DNase I pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.11
-
 pro-tripeptidyl peptidase pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.11
-
 pro-tripeptidyl peptidase pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.352
-
 NPC2 pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.367
-
 NPC2 pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.5
-
 RNase B pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.556
-
 RNase B pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
1
-
 soybean agglutinin pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
1
-
 soybean agglutinin pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
3.3
-
 Man9GlcNAc1 oligosaccharide pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
5.7
-
 Man9GlcNAc1 oligosaccharide pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
33
-
 methyl alpha-D-mannopyranoside pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
48
-
 methyl alpha-D-mannopyranoside pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
 alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
330000
-
 alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetyl-D-glucosamine + lysosomal-enzyme D-mannose = UMP + lysosomal-enzyme N-acetyl-D-glucosaminyl-phospho-D-mannose the alpa/beta subunits of GlcNAc-1-phosphotransferase recognize and bind the conformation-dependent protein determinant of acid hydrolases as well as critical lysines. The subunits then mediate the catalytic function of the transferase. Interaction of the alpa/beta subunits with the protein determinant of acid hydrolases stimulates the catalytic function of the transferase. The gamma subunit enhances the rate of GlcNAc-P transfer to the oligosaccharides of acid hydrolase acceptors. The requirement for the gamma subunit to achieve optimal levels of phosphorylation varies greatly among the different acid hydrolases, and in some instances the gamma subunit is dispensable Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + cathepsin D
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + DNase I
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + Man9GlcNAc1 oligosaccharide
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + methyl alpha-D-mannopyranoside
-
 Homo sapiens UMP + methyl (6-O-alpha-N-acetyl-D-glucosaminylphospho)-alpha-D-mannopyranoside
-
 ?
UDP-N-acetyl-D-glucosamine + NPC2
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + pro-tripeptidyl peptidase
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + RNase B
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + soybean agglutinin
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + uteroferrin
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
GlcNAc-1-phosphotransferase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0041
-
 soybean agglutinin pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.0045
-
 DNase I pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.005
-
 DNase I pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.005
-
 soybean agglutinin pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.008
-
 pro-tripeptidyl peptidase pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.011
-
 uteroferrin pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.03
-
 cathepsin D pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.033
-
 uteroferrin pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.038
-
 pro-tripeptidyl peptidase pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.042
-
 RNase B pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.05
-
 RNase B pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.11
-
 cathepsin D pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.44
-
 NPC2 pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.5
-
 methyl alpha-D-mannopyranoside pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.62
-
 NPC2 pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.75
-
 Man9GlcNAc1 oligosaccharide pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.75
-
 methyl alpha-D-mannopyranoside pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
1.55
-
 Man9GlcNAc1 oligosaccharide pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction 30% of the acid hydrolases of gamma gene knockout mice brain are phosphorylated at levels equivalent to that in wild-type brain, although 25% are poorly phosphorylated compared to wild-type. The rest of the acid hydrolases of the gamma-deficient sample are phosphorylated at an intermediate level. This shows that some acid hydrolases are highly dependent on the presence of the gamma subunit to acquire the Man-6-P, although others are well phosphorylated by the alpha/beta subunits alone Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.005
-
 soybean agglutinin pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.005
-
 soybean agglutinin pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.0166
-
 methyl alpha-D-mannopyranoside pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.0166
-
 methyl alpha-D-mannopyranoside pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.075
-
 pro-tripeptidyl peptidase pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.08
-
 DNase I pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.09
-
 RNase B pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.09
-
 RNase B pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.15
-
 DNase I pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.23
-
 Man9GlcNAc1 oligosaccharide pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
0.266
-
 Man9GlcNAc1 oligosaccharide pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.36
-
 pro-tripeptidyl peptidase pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
0.45
-
 uteroferrin pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
1
-
 uteroferrin pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
1.2
-
 cathepsin D pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
1.25
-
 NPC2 pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase Homo sapiens
1.7
-
 NPC2 pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens
4.45
-
 cathepsin D pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase Homo sapiens