Literature summary for 2.7.8.17 extracted from

Lu, J.; Takahashi, T.; Ohoka, A.; Nakajima, K.; Hashimoto, R.; Miura, N.; Tachikawa, H.; Gao, X.D.
Alg14 organizes the formation of a multiglycosyltransferase complex involved in initiation of lipid-linked oligosaccharide biosynthesis (2012), Glycobiology, 22, 504-516.

Search for more literature for 2.7.8.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
DELTA1-47	N-terminal region (residues 1-47) of Saccharomyces cerevisiae Alg14 localizes its green fluorescent protein fusion to the endoplasmic reticulum membrane. Coimmunoprecipitation demonstrates that the N-terminal region of Alg14 is required for direct interaction with Alg7	Saccharomyces cerevisiae
G163A/G165A	mutant fails to rescue a loss of Alg14 function. Mutant is inactivated by loss of conserved G-loop	Saccharomyces cerevisiae
V131A/I135A/V139A/V143A/V146A/F150A	a mutant in which six hydrophobic residues are replaced with L-Ala is able to rescue the loss of Alg14 function, indicating that the mutated hydrophobic residues do not have a deleterious effect on Alg14 activity. Growth of these cells is extremely slow at 30°C	Saccharomyces cerevisiae
V131I/I135L/V139I/V143I/V146I/F150L	a mutant in which six hydrophobic residues are replaced with L-Ala is able to rescue the loss of Alg14 function	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	N-terminal region of Alg14 is respnsible for its localization to the endoplasmic reticulum membrane	Saccharomyces cerevisiae	5783	-

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Synonyms

Synonyms	Comment	Organism
Alg14	-	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
malfunction	deletion of the Alg14 N-terminal region causes a severe growth defect at high temperature. Malfunction can be partially complemented by overexpression of Alg7	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)