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Literature summary for 2.7.8.17 extracted from

  • Bao, M.; Elmendorf, B.J.; Booth, J.L.; Drake, R.R.; Canfield, W.M.
    Bovine UDP-N-acetylglucosamine:lysosomal-enzyme N-acetylglucosamine-1-phosphotransferase. II. Enzymatic characterization and identification of the catalytic subunit (1996), J. Biol. Chem., 271, 31446-31451.
    View publication on PubMed
Search for more literature for 2.7.8.17

Activating Compound

Activating Compound Comment Organism Structure
D-glucose 6-phosphate activates Bos taurus
D-mannose 6-phosphate activates Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
ADP 5 mM, 39% inhibition Bos taurus
ATP 5 mM, 59% inhibition Bos taurus
EDTA 10 mM, complete inhibition Bos taurus
additional information selective inhibition of cathepsin D phosphorylation by rabbit anti-bovine GlcNAc phosphotransferase Bos taurus
Sodium phosphate 5 mM, pH 7.0, 55% inhibition Bos taurus
UDP 5 mM, 74% inhibition Bos taurus
UDPglucose 5 mM, 66% inhibition; competitive Bos taurus
UTP 5 mM, 34% inhibition Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 cathepsin D pH 7.4 Bos taurus
0.022
-
 uteroferrin pH 7.6 Bos taurus
0.03
-
 UDP-GlcNAc pH 7.6 Bos taurus
0.064
-
 methyl-alpha-D-mannoside pH 7.6 Bos taurus
1.244
-
 ribonuclease B pH 7.6 Bos taurus
63.7
-
 methyl-alpha-D-mannoside pH 7.6 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ strictly dependent on Mg2+ or Mn2+ Bos taurus
Mn2+ Km: 0.185 mM, strictly dependent on Mg2+ or Mn2+ Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 2 * 166000 + 2 * 51000 + 2 * 56000, a 540000 Da enzyme complex is composed of disulfide-linked homodimers of 166000 and 51000 Da subunits and two identical, noncovalently associated 56000 Da subunits, SDS-PAGE Bos taurus
56000
-
 2 * 166000 + 2 * 51000 + 2 * 56000, a 540000 Da enzyme complex is composed of disulfide-linked homodimers of 166000 and 51000 Da subunits and two identical, noncovalently associated 56000 Da subunits, SDS-PAGE Bos taurus
166000
-
 2 * 166000 + 2 * 51000 + 2 * 56000, a 540000 Da enzyme complex is composed of disulfide-linked homodimers of 166000 and 51000 Da subunits and two identical, noncovalently associated 56000 Da subunits, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + cathepsin D
-
 Bos taurus UMP + ?
-
 ?
UDP-N-acetyl-D-glucosamine + methyl-alpha-D-mannoside
-
 Bos taurus UMP + N-acetyl-D-glucosamine-phospho-(methyl-alpha-D-mannoside)
-
 ?
UDP-N-acetyl-D-glucosamine + ribonuclease B
-
 Bos taurus UMP + ?
-
 ?
UDP-N-acetyl-D-glucosamine + UDP-glucose
-
 Bos taurus UMP + ?
-
 ?
UDP-N-acetyl-D-glucosamine + uteroferrin
-
 Bos taurus UMP + ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 2 * 166000 + 2 * 51000 + 2 * 56000, a 540000 Da enzyme complex is composed of disulfide-linked homodimers of 166000 and 51000 Da subunits and two identical, noncovalently associated 56000 Da subunits, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6 7.5
-
 Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.733
-
 UDPglucose pH 7.6 Bos taurus