Any feedback?
Literature summary for 2.7.8.17 extracted from
- UDP-N-acetylglucosamine: lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (1984), Methods Enzymol., 107, 163-172.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP | - |
Homo sapiens |  |
| UDP | 5 mM, 63% inhibition | Rattus norvegicus | |
| UDPglucose | competitive with respect to UDP-GlcNAc | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
UDP-GlcNAc | pH 6.5, 37°C | Rattus norvegicus | |
| 183 | - |
methyl-alpha-D-mannoside | pH 6.5, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | - |
Mus musculus | 5794 | - |
| Golgi apparatus | - |
Homo sapiens | 5794 | - |
| Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
| Golgi membrane | integral membrane protein | Rattus norvegicus | 139 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
| Mg2+ | the divalent cation requirement is satisfied by Mn2+ better than by Mg2+ | Rattus norvegicus | |
| Mn2+ | - |
Homo sapiens | |
| Mn2+ | the divalent cation requirement is satisfied by Mn2+ better than by Mg2+ | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Ovis aries | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| BW5147 cell | - |
Mus musculus | - |
| fibroblast | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Ovis aries | - |
| P388D1 cell | - |
Mus musculus | - |
| peripheral blood mononuclear cell | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000733 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 2 months, less than 10% loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Homo sapiens | ? | - |
? | |
| additional information | UDPglucose is also a substrate with a catalytic efficiency about 12fold worse than UDP-GlcNAc. The enzyme phosphorylates lysosomal enzymes in an in vitro assay at least 100fold more efficiently than either other glycoproteins with similar carbohydrate chains or free oligosaccharides | Rattus norvegicus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + methyl-alpha-D-mannoside | - |
Rattus norvegicus | UMP + N-acetyl-D-glucosamine-phospho-(methyl-alpha-D-mannoside) | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | with Tris-HCl giving 50% more activity than sodium cacodylate | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
