General Stability | Organism |
---|---|
a 1:4 dilution leads to a 65% loss of activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tunicamycin | 1 mM, complete inhibition | Saccharomyces cerevisiae | |
UDP | 1 mM, 55% inhibition | Saccharomyces cerevisiae | |
UMP | 1 mM, 45% inhibition | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
dolichyl phosphate | pH 7.5 | Saccharomyces cerevisiae | |
0.015 | - |
UDP-GlcNAc | pH 7.5 | Saccharomyces cerevisiae | |
0.18 | - |
dolichyl phosphate | pH 7.5 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
microsome | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent metal required, Ca2+ is less effective than Mg2+ | Saccharomyces cerevisiae | |
Mg2+ | divalent metal required, optimal activity in presence of Mg2+ | Saccharomyces cerevisiae | |
Mn2+ | divalent metal required, Mn2+ is less effective than Mg2+ | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + dolichyl phosphate | Saccharomyces cerevisiae | first enzyme of dolichol pathway | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + dolichyl phosphate | Saccharomyces cerevisiae X2180-1A | first enzyme of dolichol pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
X2180-1A | - |
Saccharomyces cerevisiae X2180-1A | - |
X2180-1A | - |
Storage Stability | Organism |
---|---|
-20°C, solubilized enzyme loses 96% of its activity after 6 days, only 20% loss of activity after 6 days in presence of 0.07 mM UDP-GlcNAc | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | - |
Saccharomyces cerevisiae | UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | - |
Saccharomyces cerevisiae X2180-1A | UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol | - |
? | |
UDP-N-acetyl-D-glucosamine + dolichyl phosphate | first enzyme of dolichol pathway | Saccharomyces cerevisiae | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + dolichyl phosphate | first enzyme of dolichol pathway | Saccharomyces cerevisiae X2180-1A | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 60% of maximal activity, pH 8.5: about 35% of maximal activity | Saccharomyces cerevisiae |