BRENDA - Enzyme Database
show all sequences of 2.7.8.14

The synthesis of polyribitol phosphate. I. Purification of polyribitol phosphate polymerase and lipoteichoic acid carrier

Fiedler, F.; Glaser, L.; J. Biol. Chem. 249, 2684-2689 (1974)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
cardiolipin
stimulates 6fold at 0.36 mM
Staphylococcus aureus
lipoteichoic acid
required as carrier
Staphylococcus aureus
phosphatidylcholine
stimulates 2.7fold 0.36 mM
Staphylococcus aureus
phosphatidylethanolamine
stimulates 5fold 0.36 mM
Staphylococcus aureus
phosphatidylglycerol
stimulates 4fold 0.36 mM
Staphylococcus aureus
Phospholipid
required
Staphylococcus aureus
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-hydroxymercuribenzoate
inhibition at 0.4 mM, can be reversed by 2-mercaptoethanol
Staphylococcus aureus
CDPglycerol
weak
Staphylococcus aureus
CMP
-
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CDPribitol + (ribitol phosphate)n
Staphylococcus aureus
-
CMP + (ribitol phosphate)n+1
-
-
?
CDPribitol + (ribitol phosphate)n
Staphylococcus aureus H
-
CMP + (ribitol phosphate)n+1
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Staphylococcus aureus
-
H
-
Staphylococcus aureus H
-
H
-
Purification (Commentary)
Commentary
Organism
400fold
Staphylococcus aureus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0035
-
-
Staphylococcus aureus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CDPribitol + (ribitol phosphate)n
-
645346
Staphylococcus aureus
CMP + (ribitol phosphate)n+1
-
-
-
?
CDPribitol + (ribitol phosphate)n
-
645346
Staphylococcus aureus H
CMP + (ribitol phosphate)n+1
-
-
-
?
CDPribitol + lipoteichoic acid
-
645346
Staphylococcus aureus
lipoteichoic acid-(P-ribitol)n + CMP
-
-
-
-
CDPribitol + lipoteichoic acid
-
645346
Staphylococcus aureus H
lipoteichoic acid-(P-ribitol)n + CMP
-
-
-
-
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
24 h, very stable
Staphylococcus aureus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
cardiolipin
stimulates 6fold at 0.36 mM
Staphylococcus aureus
lipoteichoic acid
required as carrier
Staphylococcus aureus
phosphatidylcholine
stimulates 2.7fold 0.36 mM
Staphylococcus aureus
phosphatidylethanolamine
stimulates 5fold 0.36 mM
Staphylococcus aureus
phosphatidylglycerol
stimulates 4fold 0.36 mM
Staphylococcus aureus
Phospholipid
required
Staphylococcus aureus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-hydroxymercuribenzoate
inhibition at 0.4 mM, can be reversed by 2-mercaptoethanol
Staphylococcus aureus
CDPglycerol
weak
Staphylococcus aureus
CMP
-
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CDPribitol + (ribitol phosphate)n
Staphylococcus aureus
-
CMP + (ribitol phosphate)n+1
-
-
?
CDPribitol + (ribitol phosphate)n
Staphylococcus aureus H
-
CMP + (ribitol phosphate)n+1
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
400fold
Staphylococcus aureus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0035
-
-
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CDPribitol + (ribitol phosphate)n
-
645346
Staphylococcus aureus
CMP + (ribitol phosphate)n+1
-
-
-
?
CDPribitol + (ribitol phosphate)n
-
645346
Staphylococcus aureus H
CMP + (ribitol phosphate)n+1
-
-
-
?
CDPribitol + lipoteichoic acid
-
645346
Staphylococcus aureus
lipoteichoic acid-(P-ribitol)n + CMP
-
-
-
-
CDPribitol + lipoteichoic acid
-
645346
Staphylococcus aureus H
lipoteichoic acid-(P-ribitol)n + CMP
-
-
-
-
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
24 h, very stable
Staphylococcus aureus
Other publictions for EC 2.7.8.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
645345
Fischer
Structural requirements of lip ...
Staphylococcus aureus, Staphylococcus aureus H
J. Biol. Chem.
255
4550-4556
1980
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
645346
Fiedler
The synthesis of polyribitol p ...
Staphylococcus aureus, Staphylococcus aureus H
J. Biol. Chem.
249
2684-2689
1974
6
-
-
-
-
-
3
-
-
-
-
2
-
5
-
-
1
-
-
-
1
-
4
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
1
-
-
1
-
4
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
645347
Fiedler
The synthesis of polyribitol p ...
Staphylococcus aureus, Staphylococcus aureus H
J. Biol. Chem.
249
2690-2695
1974
-
-
-
-
-
-
1
-
-
-
-
2
-
5
-
-
-
1
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
645344
Ishimoto
Polyribitol phosphate syntheta ...
Staphylococcus aureus
J. Biol. Chem.
241
639-650
1966
-
-
-
-
-
-
5
1
1
4
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
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-
-
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-
-
-
-
-
-
-
5
-
1
1
4
-
1
-
-
-
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-
-
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-
2
-
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-
1
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