BRENDA - Enzyme Database show
show all sequences of 2.7.8.13

Genetic analysis of MraY inhibition by the {varphi}X174 protein E

Zheng, Y.; Struck, D.K.; Bernhardt, T.G.; Young, R.; Genetics 180, 1459-1466 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
in medium-copy plasmid pBAD30 for complementation studies in Escherichia coli K-12 mutant strain RY3321 lacking endogenous MraY
Bacillus subtilis
Engineering
Amino acid exchange
Commentary
Organism
D231N
catalytically inactive, overexpression does not rescue bacteria from protein E-induced lysis
Bacillus subtilis
D267N
enzymatically inactive, overexpression rescues bacteria from protein E-induced lysis
Escherichia coli
deltaL172
single-codon deletion in putative transmembrane domain TMD5, mediates PhiX174-resistancy only in catalytically active form and at high culture densitiy
Escherichia coli
F288L
missense mutation in putative transmembrane domain TMD9, mediates PhiX174-resistancy only in catalytically active form
Escherichia coli
G168S
mediates PhiX174-resistancy only at high culture densitiy, similar to wild-type
Escherichia coli
P170L
mediates PhiX174-resistancy only in catalytically active form and at high culture densitiy, interacts with Epos gene product more strongly than with protein E
Escherichia coli
V291M
mediates PhiX174-resistancy only at high culture densitiy, similar to wild-type
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
protein E
lysis protein of bacteriophage PhiX174, (strong) binding to MraY leads to cell lysis, overexpression of MraY wild-type rescues bacteria from protein E-induced lysis
Bacillus subtilis
protein E
lysis protein of bacteriophage PhiX174, binding to MraY leads to cell lysis, overexpression of MraY wild-type rescues bacteria from protein E-induced lysis
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
Escherichia coli
Lipid I precursor synthesis, murein biosynthesis
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
-
-
?
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
Bacillus subtilis
Lipid I precursor synthesis, murein biosynthesis
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Bacillus subtilis
Q03521
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
Lipid I precursor synthesis, murein biosynthesis
692509
Escherichia coli
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
-
-
-
?
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
Lipid I precursor synthesis, murein biosynthesis
692509
Bacillus subtilis
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
-
-
-
?
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
efficient complementation of enzymatic activity in bacterial strain lacking endogenous MraY
692509
Bacillus subtilis
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
in medium-copy plasmid pBAD30 for complementation studies in Escherichia coli K-12 mutant strain RY3321 lacking endogenous MraY
Bacillus subtilis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D231N
catalytically inactive, overexpression does not rescue bacteria from protein E-induced lysis
Bacillus subtilis
D267N
enzymatically inactive, overexpression rescues bacteria from protein E-induced lysis
Escherichia coli
deltaL172
single-codon deletion in putative transmembrane domain TMD5, mediates PhiX174-resistancy only in catalytically active form and at high culture densitiy
Escherichia coli
F288L
missense mutation in putative transmembrane domain TMD9, mediates PhiX174-resistancy only in catalytically active form
Escherichia coli
G168S
mediates PhiX174-resistancy only at high culture densitiy, similar to wild-type
Escherichia coli
P170L
mediates PhiX174-resistancy only in catalytically active form and at high culture densitiy, interacts with Epos gene product more strongly than with protein E
Escherichia coli
V291M
mediates PhiX174-resistancy only at high culture densitiy, similar to wild-type
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
protein E
lysis protein of bacteriophage PhiX174, (strong) binding to MraY leads to cell lysis, overexpression of MraY wild-type rescues bacteria from protein E-induced lysis
Bacillus subtilis
protein E
lysis protein of bacteriophage PhiX174, binding to MraY leads to cell lysis, overexpression of MraY wild-type rescues bacteria from protein E-induced lysis
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
Escherichia coli
Lipid I precursor synthesis, murein biosynthesis
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
-
-
?
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
Bacillus subtilis
Lipid I precursor synthesis, murein biosynthesis
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
Lipid I precursor synthesis, murein biosynthesis
692509
Escherichia coli
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
-
-
-
?
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
Lipid I precursor synthesis, murein biosynthesis
692509
Bacillus subtilis
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
-
-
-
?
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
efficient complementation of enzymatic activity in bacterial strain lacking endogenous MraY
692509
Bacillus subtilis
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
-
-
-
?
Other publictions for EC 2.7.8.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737788
Al-Dabbagh
Catalytic mechanism of MraY an ...
Bacillus subtilis 168, Bacillus subtilis
Biochimie
127
249-257
2016
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1
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2
1
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1
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7
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12
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12
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1
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1
1
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2
2
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737580
Moberg
Assessing HTS performance usin ...
Bacillus subtilis 168, Bacillus subtilis
Assay Drug Dev. Technol.
12
506-513
2014
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2
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738596
Ishizaki
Inhibition of the first step i ...
Bacillus subtilis 168, Bacillus subtilis
J. Biol. Chem.
288
30309-30319
2013
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6
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1
1
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725566
Shapiro
A high-throughput, homogeneous ...
Escherichia coli
J. Biomol. Screen.
17
662-672
2012
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690996
Al-Dabbagh
Active site mapping of MraY, a ...
Bacillus subtilis
Biochemistry
47
8919-8928
2008
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1
1
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14
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27
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1
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3
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1
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15
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32
2
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14
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15
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3
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32
2
1
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692509
Zheng
Genetic analysis of MraY inhib ...
Bacillus subtilis, Escherichia coli
Genetics
180
1459-1466
2008
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7
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694514
Xu
Synthesis of gem-difluorinated ...
Micrococcus flavus
Org. Biomol. Chem.
6
157-161
2008
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704218
Murakami
A-94964, a novel inhibitor of ...
Enterococcus faecalis, Staphylococcus aureus
J. Antibiot.
61
537-544
2008
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2
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6
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2
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704219
Fujita
A-94964, novel inhibitor of ba ...
Enterococcus faecalis, Staphylococcus aureus
J. Antibiot.
61
545-549
2008
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2
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2
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676084
Mohammadi
The essential peptidoglycan gl ...
Escherichia coli, Escherichia coli LMC500
Mol. Microbiol.
65
1106-1121
2007
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692188
Le Corre
-
Towards new MraY inhibitors: a ...
Bacteria
Eur. J. Org. Chem.
2007
5386-5394
2007
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695173
Kurosu
-
Chemoenzymatic synthesis of Pa ...
Bacteria
Tetrahedron Lett.
48
799-803
2007
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675899
Schouten
Fluorescent reagents for in vi ...
Escherichia coli, Escherichia coli DH5-alpha
Mol. Biosyst.
2
484-491
2006
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1
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15
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10
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660721
Stachyra
Fluorescence detection-based f ...
Escherichia coli
Antimicrob. Agents Chemother.
48
897-902
2004
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1
1
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18
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662009
Lloyd
Phospho-N-acetyl-muramyl-penta ...
Escherichia coli
J. Bacteriol.
186
1747-1757
2004
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662239
Bouhss
Purification and characterizat ...
Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Thermotoga maritima
J. Biol. Chem.
279
29974-29980
2004
1
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4
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645343
Zawadzke
Targeting the MraY and MurG ba ...
Escherichia coli
Anal. Biochem.
314
243-252
2003
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645342
Azzolina
The cell wall and cell divisio ...
Pseudomonas aeruginosa
Protein Expr. Purif.
21
393-400
2001
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1
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645341
Bouhss
Topological analysis of the Mr ...
Escherichia coli, Staphylococcus aureus
Mol. Microbiol.
34
576-585
1999
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4
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645340
Brandish
Slow binding inhibition of pho ...
Escherichia coli, Escherichia coli JM105
J. Biol. Chem.
271
7609-7614
1996
1
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1
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3
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8
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12
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1
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12
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645339
Pickup
-
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