BRENDA - Enzyme Database show
show all sequences of 2.7.8.13

Purification and characterization of the bacterial MraY translocase catalyzing the first membrane step of peptidoglycan biosynthesis

Bouhss, A.; Crouvoisier, M.; Blanot, D.; Mengin-Lecreulx, D.; J. Biol. Chem. 279, 29974-29980 (2004)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
N-lauroyl sarcosine is the best detergent for translocase activity, among different detergents tested CHAPS is the worst for translocase activity
Bacillus subtilis
Cloned(Commentary)
Commentary
Organism
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha
Bacillus subtilis
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha
Escherichia coli
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha
Staphylococcus aureus
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha
Thermotoga maritima
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
N-lauroyl sarcosine is the best detergent for translocase activity, among different detergents tested CHAPS is the worst for translocase activity
Bacillus subtilis
tunicamycin
IC50: 0.012 mM
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
undecaprenyl phosphate
-
Bacillus subtilis
1
-
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)
-
Bacillus subtilis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
integral membrane protein
Bacillus subtilis
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolute requirement for divalent cations, optimal activity with Mg2+ at concentrations 40-75 mM
Bacillus subtilis
Mn2+
enzyme activity in presence of Mn2+ is 1% of the activity detected in presence of Mg2+
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
Bacillus subtilis
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
Escherichia coli
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
Thermotoga maritima
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
r
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
Staphylococcus aureus
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
recombinant
-
Escherichia coli
-
recombinant
-
Staphylococcus aureus
-
recombinant
-
Thermotoga maritima
-
recombinant
-
Purification (Commentary)
Commentary
Organism
-
Bacillus subtilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
662239
Bacillus subtilis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
662239
Escherichia coli
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
662239
Thermotoga maritima
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
662239
Bacillus subtilis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
662239
Escherichia coli
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
662239
Thermotoga maritima
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
662239
Staphylococcus aureus
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
662239
Staphylococcus aureus
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.33
-
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)
-
Bacillus subtilis
5.33
-
undecaprenyl phosphate
-
Bacillus subtilis
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.012
-
IC50: 0.012 mM
Bacillus subtilis
tunicamycin
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
N-lauroyl sarcosine is the best detergent for translocase activity, among different detergents tested CHAPS is the worst for translocase activity
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha
Bacillus subtilis
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha
Escherichia coli
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha
Staphylococcus aureus
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha
Thermotoga maritima
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.012
-
IC50: 0.012 mM
Bacillus subtilis
tunicamycin
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
N-lauroyl sarcosine is the best detergent for translocase activity, among different detergents tested CHAPS is the worst for translocase activity
Bacillus subtilis
tunicamycin
IC50: 0.012 mM
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
undecaprenyl phosphate
-
Bacillus subtilis
1
-
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)
-
Bacillus subtilis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
integral membrane protein
Bacillus subtilis
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolute requirement for divalent cations, optimal activity with Mg2+ at concentrations 40-75 mM
Bacillus subtilis
Mn2+
enzyme activity in presence of Mn2+ is 1% of the activity detected in presence of Mg2+
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
Bacillus subtilis
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
Escherichia coli
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
Thermotoga maritima
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
r
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
Staphylococcus aureus
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
662239
Bacillus subtilis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
662239
Escherichia coli
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
662239
Thermotoga maritima
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
662239
Bacillus subtilis
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
662239
Escherichia coli
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
662239
Thermotoga maritima
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
662239
Staphylococcus aureus
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
662239
Staphylococcus aureus
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.33
-
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)
-
Bacillus subtilis
5.33
-
undecaprenyl phosphate
-
Bacillus subtilis
Other publictions for EC 2.7.8.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737788
Al-Dabbagh
Catalytic mechanism of MraY an ...
Bacillus subtilis 168, Bacillus subtilis
Biochimie
127
249-257
2016
-
1
1
-
1
-
2
1
1
1
-
2
-
7
-
-
-
1
-
-
-
-
12
-
1
-
-
1
1
-
-
-
-
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1
-
1
1
-
-
1
-
1
2
-
1
1
1
-
2
-
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-
-
-
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-
-
12
-
1
-
-
1
1
-
-
-
-
2
2
-
-
-
737580
Moberg
Assessing HTS performance usin ...
Bacillus subtilis 168, Bacillus subtilis
Assay Drug Dev. Technol.
12
506-513
2014
-
2
-
-
-
-
1
-
-
1
-
-
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4
-
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-
-
-
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4
-
1
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1
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2
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1
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1
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-
4
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1
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1
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-
-
-
-
-
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738596
Ishizaki
Inhibition of the first step i ...
Bacillus subtilis 168, Bacillus subtilis
J. Biol. Chem.
288
30309-30319
2013
-
-
-
-
-
-
3
-
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-
2
-
6
-
-
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2
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2
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2
3
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2
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2
-
-
-
-
-
-
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-
-
1
1
-
-
-
725566
Shapiro
A high-throughput, homogeneous ...
Escherichia coli
J. Biomol. Screen.
17
662-672
2012
-
-
-
-
-
-
1
-
-
1
-
-
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2
-
-
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-
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1
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1
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1
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1
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690996
Al-Dabbagh
Active site mapping of MraY, a ...
Bacillus subtilis
Biochemistry
47
8919-8928
2008
-
1
1
-
14
-
-
27
-
1
-
-
-
3
-
-
1
-
-
-
15
-
3
-
-
-
-
32
2
1
-
-
-
-
-
-
1
1
-
-
14
-
-
-
-
27
-
1
-
-
-
-
-
1
-
-
15
-
3
-
-
-
-
32
2
1
-
-
-
-
-
-
-
-
692509
Zheng
Genetic analysis of MraY inhib ...
Bacillus subtilis, Escherichia coli
Genetics
180
1459-1466
2008
-
-
1
-
7
-
2
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
3
-
-
-
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-
-
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1
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7
-
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2
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2
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-
-
3
-
-
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-
-
-
-
-
-
-
-
-
694514
Xu
Synthesis of gem-difluorinated ...
Micrococcus flavus
Org. Biomol. Chem.
6
157-161
2008
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704218
Murakami
A-94964, a novel inhibitor of ...
Enterococcus faecalis, Staphylococcus aureus
J. Antibiot.
61
537-544
2008
-
2
-
-
-
-
2
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
2
-
-
-
2
-
-
-
-
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2
-
2
-
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2
2
-
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2
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4
-
2
-
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2
-
-
-
-
2
2
-
-
-
704219
Fujita
A-94964, novel inhibitor of ba ...
Enterococcus faecalis, Staphylococcus aureus
J. Antibiot.
61
545-549
2008
-
2
-
-
-
-
2
-
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2
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3
-
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2
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2
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2
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2
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