Literature summary for 2.7.8.13 extracted from

Stachyra, T.; Dini, C.; Ferrari, P.; Bouhss, A.; Van Heijenoort, J.; Mengin-Lecreulx, D.; Blanot, D.; Biton, J.; Le Beller, D.
Fluorescence detection-based functional assay for high-throughput screening for MraY (2004), Antimicrob. Agents Chemother., 48, 897-902.

Search for more literature for 2.7.8.13

Application

Application	Comment	Organism
medicine	the enzyme is a potential target for antibacterial compounds	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
mureidomycin B	IC50: 0.000038 mM	Escherichia coli
RU66950	IC50: 0.07 mM	Escherichia coli
RU70157	IC50: 0.0054 mM	Escherichia coli
RU72143	IC50: 0.0015 mM	Escherichia coli
RU72839	IC50: 0.0003 mM	Escherichia coli
RU74709	IC50: 0.000096 mM	Escherichia coli
RU74854	IC50: 0.000022 mM	Escherichia coli
RU75413	IC50: 0.000033 mM	Escherichia coli
RU76491	IC50: 0.000084 mM	Escherichia coli
RU76492	IC50: 0.0001 mM	Escherichia coli
RU77254	IC50: 0.000027 mM	Escherichia coli
RU78695	IC50: 0.000034 mM	Escherichia coli
RU80303	IC50: 0.0005 mM	Escherichia coli
RU88108	IC50: 0.000051 mM	Escherichia coli
RU88110	IC50: 0.000016 mM	Escherichia coli
RU88114	IC50: 0.000084 mM	Escherichia coli
RU88155	IC50: 0.0015 mM	Escherichia coli
tunicamycin	IC50: 0.0019 mM	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate	Escherichia coli	catalyzes the first membrane step in biosynthesis of bacterial cell wall peptidoglycan	UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	assay for MraY by using UDP-MurNAc-Nepsilon-dansylpentapeptide, a fluorescent derivative of the MraY nucleotide substrate. Two assay versions are developed, one consisting of the high-pressure liquid chromatography separation of the substrate and product and the other, without separation and adapted to the high-throughput format, taking advantage of the different fluorescent properties of the nucleotide and lipid I in the reaction medium	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate	catalyzes the first membrane step in biosynthesis of bacterial cell wall peptidoglycan	Escherichia coli	UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol	-	?

Synonyms

Synonyms	Comment	Organism
MraY	-	Escherichia coli

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.000016	-	IC50: 0.000016 mM	Escherichia coli	RU88110
0.000022	-	IC50: 0.000022 mM	Escherichia coli	RU74854
0.000027	-	IC50: 0.000027 mM	Escherichia coli	RU77254
0.000033	-	IC50: 0.000033 mM	Escherichia coli	RU75413
0.000034	-	IC50: 0.000034 mM	Escherichia coli	RU78695
0.000038	-	IC50: 0.000038 mM	Escherichia coli	mureidomycin B
0.000051	-	IC50: 0.000051 mM	Escherichia coli	RU88108
0.000084	-	IC50: 0.000084 mM	Escherichia coli	RU76491
0.000084	-	IC50: 0.000084 mM	Escherichia coli	RU88114
0.000096	-	IC50: 0.000096 mM	Escherichia coli	RU74709
0.0001	-	IC50: 0.0001 mM	Escherichia coli	RU76492
0.0003	-	IC50: 0.0003 mM	Escherichia coli	RU72839
0.0005	-	IC50: 0.0005 mM	Escherichia coli	RU80303
0.0015	-	IC50: 0.0015 mM	Escherichia coli	RU88155
0.0015	-	IC50: 0.0015 mM	Escherichia coli	RU72143
0.0019	-	IC50: 0.0019 mM	Escherichia coli	tunicamycin
0.0054	-	IC50: 0.0054 mM	Escherichia coli	RU70157
0.07	-	IC50: 0.07 mM	Escherichia coli	RU66950

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)