Literature summary for 2.7.8.13 extracted from

Brandish, P.E.; Burnham, M.K.; Lonsdale, J.T.; Southgate, R.; Inukai, M.; Bugg, T.D.H.
Slow binding inhibition of phospho-N-acetylmuramyl-pentapeptide-translocase (Escherichia coli) by mureidomycin A (1996), J. Biol. Chem., 271, 7609-7614.

Search for more literature for 2.7.8.13

Activating Compound

Activating Compound	Comment	Organism	Structure
phosphatidylglycerol	activity is stimulated 5-10fold by inclusion of 0.1 mg/ml in both radiochemical and fluorescence enhancement assays	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in recombinant Escherichia coli JM109	Escherichia coli

General Stability

General Stability	Organism
no loss of activity observed upon gel filtration, desalted enzyme is completely inactive	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
mureidomycin A	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.013	-	dodecaprenylphosphate	pH 7.5, 30°C	Escherichia coli
0.019	-	dansyl-UDP-NAc-muramoyl-pentapeptide	pH 7.5, 30°C	Escherichia coli
0.019	-	heptaprenylphosphate	pH 7.5, 30°C	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	can be replaced only by Mn2+	Escherichia coli
additional information	no activity is observed using Ni2+, Ca2+ and Zn2+	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	45000	SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-MurNAc-pentapeptide + undecaprenyl-phosphate	Escherichia coli	natural acceptor	UMP + MurNAc-pentapeptide diphosphoryl undecaprenol	-	r
UDP-NAc-muramoyl-pentapeptide + acceptor	Escherichia coli	biosynthesis of peptidoglycan, first lipid intermediate	UMP + acceptor-phospho-NAc-muramoyl-pentapeptide	-	r
UDP-NAc-muramoyl-pentapeptide + acceptor	Escherichia coli JM105	biosynthesis of peptidoglycan, first lipid intermediate	UMP + acceptor-phospho-NAc-muramoyl-pentapeptide	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	wild-type JM105	-
Escherichia coli JM105	-	wild-type JM105	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	specific activity is increased 2fold by induction with isopropyl-beta-D-thiogalactopyranoside in the late logarithmic phase	Escherichia coli
additional information	-	0.42 units, using fluorescence enhancement assay, recombinant strain JM109 (pTrc99A), 11.9 units, recombinant strain JM109 (pBROC525), activity is expressed as increase in units of fluorescence emission per min at 30°C	Escherichia coli
0.000276	-	specific activity of particulate enzyme from recombinant strain JM109 (pBROC525), in absence of exogenous lipid acceptor	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dansyl-UDP-NAc-muramoyl-pentapeptide + undecaprenyl phosphate	-	Escherichia coli	UMP + dansyl-UDP-NAc-muramoyl-pentapeptide-diphosphoundecaprenol	-	?
UDP-MurNAc(oyl-L-Ala-gamma-D-Glu-meso-2,6-diaminopimelyl-(N-dansyl)-D-Ala-D-Ala) + dodecaprenyl phosphate	dPP	Escherichia coli	UMP + MurNAc(oyl-L-Ala-gamma-D-Glu-meso-2,6-diaminopimelyl-(N-dansyl)-D-Ala-D-Ala)-diphosphododecaprenol	-	r
UDP-MurNAc(oyl-L-Ala-gamma-D-Glu-meso-2,6-diaminopimelyl-(N-dansyl)-D-Ala-D-Ala) + dodecaprenyl phosphate	dPP	Escherichia coli JM105	UMP + MurNAc(oyl-L-Ala-gamma-D-Glu-meso-2,6-diaminopimelyl-(N-dansyl)-D-Ala-D-Ala)-diphosphododecaprenol	-	r
UDP-MurNAc-pentapeptide + undecaprenoid-1-ol-phosphate	-	Escherichia coli	lipid-P-P-MurNAc-pentapeptide + UMP	-	r
UDP-MurNAc-pentapeptide + undecaprenoid-1-ol-phosphate	-	Escherichia coli JM105	lipid-P-P-MurNAc-pentapeptide + UMP	-	r
UDP-MurNAc-pentapeptide + undecaprenyl-phosphate	natural acceptor	Escherichia coli	UMP + MurNAc-pentapeptide diphosphoryl undecaprenol	-	r
UDP-NAc-muramoyl-pentapeptide + acceptor	-	Escherichia coli	UMP + acceptor-phospho-NAc-muramoyl-pentapeptide	-	r
UDP-NAc-muramoyl-pentapeptide + acceptor	biosynthesis of peptidoglycan, first lipid intermediate	Escherichia coli	UMP + acceptor-phospho-NAc-muramoyl-pentapeptide	-	r
UDP-NAc-muramoyl-pentapeptide + acceptor	-	Escherichia coli JM105	UMP + acceptor-phospho-NAc-muramoyl-pentapeptide	-	r
UDP-NAc-muramoyl-pentapeptide + acceptor	biosynthesis of peptidoglycan, first lipid intermediate	Escherichia coli JM105	UMP + acceptor-phospho-NAc-muramoyl-pentapeptide	-	r
UDP-NAc-muramoyl-pentapeptide + dodecaprenyl-phosphate	dodecaprenyl phosphate is a more efficient substrate than heptaprenyl-phosphate, enzyme is selective for larger substrate which is closer in chain length to the natural substrate, undecaprenyl-phosphate	Escherichia coli	UMP + acceptor-phospho-NAc-muramoyl-pentapeptide	-	r
UDP-NAc-muramoyl-pentapeptide + dodecaprenyl-phosphate	dodecaprenyl phosphate is a more efficient substrate than heptaprenyl-phosphate, enzyme is selective for larger substrate which is closer in chain length to the natural substrate, undecaprenyl-phosphate	Escherichia coli JM105	UMP + acceptor-phospho-NAc-muramoyl-pentapeptide	-	r
UDP-NAc-muramoyl-pentapeptide + heptaprenyl-phosphate	-	Escherichia coli	UMP + acceptor-phospho-NAc-muramoyl-pentapeptide	-	r

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000036	-	mureidomycin A	pH 7.5, 30°C	Escherichia coli

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Release 2023.1 (January 2023)