Cloned (Comment) | Organism |
---|---|
N-terminally truncated construct comprising the catalytic glycerol-phosphate transferase region of the enzyme (residues 312-721) is expressed | Staphylococcus epidermidis |
Crystallization (Comment) | Organism |
---|---|
structure of Staphylococcus epidermidis TagF is determined to 2.7 A resolution from a construct that includes both the membrane-targeting region and the glycerol-phosphate polymerase domains. TagF possesses a helical region for interaction with the lipid bilayer, placing the active site at a suitable distance for access to the membrane-bound substrate | Staphylococcus epidermidis |
Protein Variants | Comment | Organism |
---|---|---|
DELTA 312-721 | truncated construct contains membrane binding region, truncated construct shows same activity as full-length wild-type | Staphylococcus epidermidis |
H444N | mutant crystallizes identical to wild-type, mutant shows no detectable activity compared to wild-type | Staphylococcus epidermidis |
H584A | mutant crystallizes identical to wild-type, mutant shows no detectable activity compared to wild-type | Staphylococcus epidermidis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Staphylococcus epidermidis | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus epidermidis | Q5HLM5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-glycerol + (glycerophosphate)n | - |
Staphylococcus epidermidis | CMP + (glycerophosphate)n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Staphylococcus epidermidis |
Synonyms | Comment | Organism |
---|---|---|
TagF | - |
Staphylococcus epidermidis |
teichoic acid polymerase | - |
Staphylococcus epidermidis |