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Literature summary for 2.7.8.12 extracted from

  • Lovering, A.L.; Lin, L.Y.; Sewell, E.W.; Spreter, T.; Brown, E.D.; Strynadka, N.C.
    Structure of the bacterial teichoic acid polymerase TagF provides insights into membrane association and catalysis (2010), Nat. Struct. Mol. Biol., 17, 582-589.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
N-terminally truncated construct comprising the catalytic glycerol-phosphate transferase region of the enzyme (residues 312-721) is expressed Staphylococcus epidermidis

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of Staphylococcus epidermidis TagF is determined to 2.7 A resolution from a construct that includes both the membrane-targeting region and the glycerol-phosphate polymerase domains. TagF possesses a helical region for interaction with the lipid bilayer, placing the active site at a suitable distance for access to the membrane-bound substrate Staphylococcus epidermidis

Protein Variants

Protein Variants Comment Organism
DELTA 312-721 truncated construct contains membrane binding region, truncated construct shows same activity as full-length wild-type Staphylococcus epidermidis
H444N mutant crystallizes identical to wild-type, mutant shows no detectable activity compared to wild-type Staphylococcus epidermidis
H584A mutant crystallizes identical to wild-type, mutant shows no detectable activity compared to wild-type Staphylococcus epidermidis

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Staphylococcus epidermidis 5886
-

Organism

Organism UniProt Comment Textmining
Staphylococcus epidermidis Q5HLM5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-glycerol + (glycerophosphate)n
-
Staphylococcus epidermidis CMP + (glycerophosphate)n+1
-
?

Subunits

Subunits Comment Organism
dimer
-
Staphylococcus epidermidis

Synonyms

Synonyms Comment Organism
TagF
-
Staphylococcus epidermidis
teichoic acid polymerase
-
Staphylococcus epidermidis