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Literature summary for 2.7.8.11 extracted from
- Phosphatidylinositol synthase of Tetrahymena: inositol isomers as substrates in phosphatidylinositol biosynthesis and headgroup exchange reactions (2007), J. Eukaryot. Microbiol., 54, 119-124.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Tetrahymena vorax | - |
- |
| microsome | microsomal fractions from Tetrahymena vorax, positive for NADPH-cytochrome c reductase activity | Tetrahymena vorax | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulation, maximum catalytic activity in presence of 2 mM MgCl2 plus 2 mM MnCl2. Less stimulatory above 5 mM | Tetrahymena vorax |  |
| Mg2+ | less effect at more than 5 mM | Tetrahymena vorax | |
| Mn2+ | stimulation, maximum catalytic activity in presence of 2 mM MgCl2 plus 2 mM MnCl2. Less stimulatory above 5 mM | Tetrahymena vorax | |
| Mn2+ | greater effect than Mg2+, less effect at more than 5 mM | Tetrahymena vorax | |
| additional information | slight or no effect by calcium, cobalt, copper, iron, lithium, molybdenum, zinc ions | Tetrahymena vorax | |
| additional information | synergistic effect of Mg2+ and Mn2+, optimum condition: 2 mM MgCl2, 2 mM MnCl2 | Tetrahymena vorax |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetrahymena vorax | - |
- |
- |
| Tetrahymena vorax V2S | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + 1D-chiro-inositol | conversion of 30.9% CDP-diacylglycerol (utilization significant compared to background, p: 0.05), microsomal fraction, 30°C, 20 min | Tetrahymena vorax | CMP + phosphatidyl-1D-chiro-inositol | - |
? | |
| CDP-diacylglycerol + 1L-chiro-inositol | conversion of 25.1% CDP-diacylglycerol (non-significant), microsomal fraction, 30°C, 20 min | Tetrahymena vorax | CMP + phosphatidyl-1L-chiro-inositol | - |
? | |
| CDP-diacylglycerol + allo-inositol | 83% of the activity on myo-inositol | Tetrahymena vorax | CMP + phosphatidyl-allo-inositol | - |
? | |
| CDP-diacylglycerol + allo-inositol | conversion of 23.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30°C, 20 min | Tetrahymena vorax | CMP + phosphatidyl-allo-inositol | - |
? | |
| CDP-diacylglycerol + allo-inositol | 83% of the activity on myo-inositol | Tetrahymena vorax V2S | CMP + phosphatidyl-allo-inositol | - |
? | |
| CDP-diacylglycerol + allo-inositol | conversion of 23.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30°C, 20 min | Tetrahymena vorax V2S | CMP + phosphatidyl-allo-inositol | - |
? | |
| CDP-diacylglycerol + D-chiro-inositol | 82% of the activity on myo-inositol | Tetrahymena vorax | CMP + phosphatidyl-chiro-inositol | - |
? | |
| CDP-diacylglycerol + epi-inositol | 62% of the activity on myo-inositol | Tetrahymena vorax | CMP + phosphatidyl-epi-inositol | - |
? | |
| CDP-diacylglycerol + epi-inositol | conversion of 31.1% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, 30°C, 20 min | Tetrahymena vorax | CMP + phosphatidyl-epi-inositol | - |
? | |
| CDP-diacylglycerol + L-chiro-inositol | 67% of the activity on myo-inositol | Tetrahymena vorax | CMP + phosphatidyl-chiro-inositol | - |
? | |
| CDP-diacylglycerol + muco-inositol | 41% of the activity on myo-inositol | Tetrahymena vorax | CMP + phosphatidyl-muco-inositol | - |
? | |
| CDP-diacylglycerol + muco-inositol | conversion of 15.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30°C, 20 min | Tetrahymena vorax | CMP + phosphatidyl-muco-inositol | - |
? | |
| CDP-diacylglycerol + myo-inositol | myo-inositol and scyllo-inositol are preferred substrates | Tetrahymena vorax | CMP + phosphatidyl-myo-inositol | - |
? | |
| CDP-diacylglycerol + myo-inositol | conversion of 37.4% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, standard reaction: 20 min, 30°C, pH 8, 2 mM MgCl2, 2 mM MnCl2, 1.6 mM Triton X-100 | Tetrahymena vorax | CMP + phosphatidyl-myo-inositol | analyses by liquid scintillation counting and thin-layer chromatography | ? | |
| CDP-diacylglycerol + scyllo-inositol | myo-inositol and scyllo-inositol are preferred substrates | Tetrahymena vorax | CMP + phosphatidyl-scyllo-inositol | - |
? | |
| CDP-diacylglycerol + scyllo-inositol | conversion of 33.7% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, 30°C, 20 min | Tetrahymena vorax | CMP + phosphatidyl-scyllo-inositol | - |
? | |
| additional information | formation of novel non-myo-inositol-containing second messengers, preferred non-myo-inositols are scyllo- and chiro-inositol | Tetrahymena vorax | ? | - |
? | |
| additional information | microsomal fraction contains additional PdtIn: inositol phosphatidyl transferase activity (inositol headgroup exchange, 20 min, 30°C) between myo-, scyllo-, 1D-chiro-inositol and phosphatidyl-myo-inositol, increased in presence of CMP, least specificity for 1L-chiro- and muco-inositol | Tetrahymena vorax | ? | - |
? | |
| additional information | myo- and scyllo-inositol preferred inositol isomers for PtdIn synthase activity, least specificity for muco-inositol | Tetrahymena vorax | ? | - |
? | |
| additional information | formation of novel non-myo-inositol-containing second messengers, preferred non-myo-inositols are scyllo- and chiro-inositol | Tetrahymena vorax V2S | ? | - |
? | |
| additional information | microsomal fraction contains additional PdtIn: inositol phosphatidyl transferase activity (inositol headgroup exchange, 20 min, 30°C) between myo-, scyllo-, 1D-chiro-inositol and phosphatidyl-myo-inositol, increased in presence of CMP, least specificity for 1L-chiro- and muco-inositol | Tetrahymena vorax V2S | ? | - |
? | |
| additional information | myo- and scyllo-inositol preferred inositol isomers for PtdIn synthase activity, least specificity for muco-inositol | Tetrahymena vorax V2S | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphatidyinositol synthase | - |
Tetrahymena vorax |
| PtdIn synthase | - |
Tetrahymena vorax |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Tetrahymena vorax |
| 30 | - |
pH 7.8 | Tetrahymena vorax |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
89% of maximum activity, pH 7.8, 10 min preincubation | Tetrahymena vorax |
| 60 | - |
82% of maximum activity, pH 7.8, 10 min preincubation | Tetrahymena vorax |
| 70 | - |
79% of maximum activity, pH 7.8, 10 min preincubation | Tetrahymena vorax |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, 80% residual activity | Tetrahymena vorax |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Tetrahymena vorax |
| 7.8 | - |
30°C | Tetrahymena vorax |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 8.4 | about 90% of maximum activity within this range | Tetrahymena vorax |
| 7.3 | 8.4 | 30°C, 90% of maximum activity | Tetrahymena vorax |
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