Application | Comment | Organism |
---|---|---|
medicine | enzymes involved in the PI biosynthetic pathway are potential antimycobacterial agents | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
tbINO, the coding sequence of ino1 amplified by PCR, cloned into expression vector pET-15b and overproduced in Escherichia coli BL21 (DE3) | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
bound to the cofactor NAD+, space group P42(1)2, cell dimensions a = 116.2, b = 116.2, c = 64.5 | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-diacylglycerol + myo-inositol | Mycobacterium tuberculosis | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-diacylglycerol + myo-inositol | - |
Mycobacterium tuberculosis | CMP + phosphatidyl-1D-myo-inositol | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mycobacterium tuberculosis |