Cloned (Comment) | Organism |
---|---|
gene PIS cloned with pUC8 and expressed in Escherichia coli HB101 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
CMP | pH 8.0, 30°C | Saccharomyces cerevisiae | |
0.066 | - |
myo-inositol | pH 8.0, 30°C, CMP-independent exchange reaction | Saccharomyces cerevisiae | |
0.09 | - |
myo-inositol | pH 8.0, 30°C, forward reaction | Saccharomyces cerevisiae | |
0.277 | - |
myo-inositol | pH 8.0, 30°C, CMP-stimulated exchange reaction | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-diacylglycerol + myo-inositol | Saccharomyces cerevisiae | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-diacylglycerol + myo-inositol | - |
Saccharomyces cerevisiae | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
additional information | enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol | Saccharomyces cerevisiae | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
optimum for exchange reaction | Saccharomyces cerevisiae |
8 | - |
- |
Saccharomyces cerevisiae |
8.5 | - |
pH optimum for reverse reaction | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | - |
Saccharomyces cerevisiae |