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Literature summary for 2.7.8.1 extracted from
- Identification and characterization of human ethanolaminephosphotransferase1 (2007), J. Lipid Res., 48, 503-508.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| fom cDNA in pET23a for expression in Escherichia coli BL21 (DE3) pLysS | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | inhibitory at concentrations higher than 5 mM | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0018 | - |
CDP-ethanolamine | pH 8.0, 37°C | Homo sapiens | |
| 1800 | - |
CDP-ethanolamine | 5 mM Mn2+, pH8, 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | or Mn2+, required. Activity increases with concentration of Mg2+ at least up to 30 mM, no activation below 5 mM | Homo sapiens | |
| Mg2+ | optimum concentration: 30 mM | Homo sapiens | |
| Mn2+ | or Mg2+, required. Maximal activity at 5 mM Mn2+ | Homo sapiens | |
| Mn2+ | optimum concentration: 5 mM | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45180 | - |
deduced from primary structure | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-ethanolamine + diacylglycerol | Homo sapiens | - |
phosphatidylethanolamine + CMP | - |
? | |
| additional information | Homo sapiens | CDP-ethanolamine-specific activity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q8WU57 | - |
- |
| Homo sapiens | Q9C0D9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| membrane precipitation by centrifugation a 100000g for 60 min | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | high expression in cerebellum | Homo sapiens | - |
| brain | tissue of highest expression level, especially in cerebellum followed by occipital pole and frontal lobe, revealed by RT-PCR and Northern blot | Homo sapiens | - |
| heart | RT-PCR and Northern blot | Homo sapiens | - |
| kidney | low expression levels, revealed by RT-PCR and Northern blot | Homo sapiens | - |
| liver | RT-PCR and Northern blot | Homo sapiens | - |
| lung | RT-PCR and Northern blot | Homo sapiens | - |
| additional information | enzyme is ubiquitously expressed in multiple tissues | Homo sapiens | - |
| pancreas | RT-PCR and Northern blot | Homo sapiens | - |
| placenta | RT-PCR and Northern blot | Homo sapiens | - |
| skeletal muscle | RT-PCR and Northern blot | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0763 | - |
5 mM Mn2+, pH 8, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-choline + diacylglycerol | only at CDP-choline concentrations higher than 25 micromolar, pH 8, 10 min, 37°C, in presence of 0.0002% Tween 20, 1 mM EGTA, 5 mM MnCl2 | Homo sapiens | phosphatidylcholine + CMP | - |
? | |
| CDP-ethanolamine + 1,2-diacylglycerol | enzyme specifically uses CDP-ethanolamine as the phosphobase donor | Homo sapiens | CMP + a phosphatidylethanolamine | - |
? | |
| CDP-ethanolamine + diacylglycerol | - |
Homo sapiens | phosphatidylethanolamine + CMP | - |
? | |
| CDP-ethanolamine + diacylglycerol | pH 8, 10 min, 37°C, in presence of 0.0002% Tween 20, 1 mM EGTA, 5 mM MnCl2 | Homo sapiens | phosphatidylethanolamine + CMP | reaction stop with 1:1 (v/v) mixture chloroform:methanol | ? | |
| additional information | CDP-ethanolamine-specific activity | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDP-ethanolamine:diacylglycerol ethanolaminephosphotransferase | - |
Homo sapiens |
| EPT | - |
Homo sapiens |
| ethanolaminephosphotransferase 1 | - |
Homo sapiens |
| hEPT1 | - |
Homo sapiens |
| hEPT1 | human EPT1, 27% amino acid sequence identity to human choline/ethanolaminephosphotransferase, hCEPT | Homo sapiens |
| selenoprotein 1 | - |
Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | deduced from primary structure | - |
6.1 |
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