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Literature summary for 2.7.7.B25 extracted from

  • Hong, Y.; Maeda, Y.; Watanabe, R.; Ohishi, K.; Mishkind, M.; Riezman, H.; Kinoshita, T.
    Pig-n, a mammalian homologue of yeast Mcd4p, is involved in transferring phosphoethanolamine to the first mannose of the glycosylphosphatidylinositol (1999), J. Biol. Chem., 274, 35099-35106 .
    View publication on PubMed
Search for more literature for 2.7.7.B25

Inhibitors

Inhibitors Comment Organism Structure
YW3548 i.e. BE49385A, a terpenoid lactone, natural inhibitor of GPI biosynthesis, inhibits transfer of phosphoethanolamine to the first mannose in mammalian cells but only slightly affects the surface expression of GPI-anchored proteins. Biosynthesis of GPI in the Pig-n knockout cells s not affected by YW3548, and yeast overexpressing MCD4 is highly resistant to YW3548 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Mus musculus 5783
-

Organism

Organism UniProt Comment Textmining
Mus musculus Q9R1S3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
F-9 cell
-
 Mus musculus
-

Synonyms

Synonyms Comment Organism
Pig-n
-
 Mus musculus
Pign
-
 Mus musculus

General Information

General Information Comment Organism
physiological function in Pig-n knockout F9 embryonal carcinoma cells, the first mannose in the GPI precursors is not modified by phosphoethanolamine. Further biosynthetic steps continue with the addition of the third mannose and the terminal phosphoethanolamine. The surface expression of Thy-1 is only partially affected. An inhibitor of GPI biosynthesis, YW3548, inhibits transfer of phosphoethanolamine to the first mannose in mammalian cells but only slightly affects the surface expression of GPI-anchored proteins Mus musculus